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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 293 results for: gne

Showing: 21 - 40 of 293 results

  1. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ... guides used in the screens they analyzed were designed to have a perfect match to the reference genome...Katherine Huang from Broad’s Pattern team, they designed a tool that could analyze CRISPR sgRNAs and report...CRISpick algorithm to prevent guides from being designed that target regions with high genomic variability...scientists to be aware of and actively use tools designed to help reduce or eliminate bias, just like the...

  2. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...most yeast vectors and many mammalian vectors are designed to shuttle between species. Figure 1: Shuttle...host’s replication machinery. A shuttle vector designed for unrelated bacteria should include oris for...target species. Fun fact! Plant vectors are usually designed to shuttle between E. coli and Agrobacterium tumefaciens...image for individual analysis. A shuttle vector designed for this approach in a mammalian cell experiment...

  3. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...reproducibility than others. Unfortunately, most ELNs are designed as closed ecosystems and act as data silos because...RSpace ELN supports reproducibility RSpace was designed in response to requirements that emerged from ... in institutional and lab servers specifically designed to hold large amounts of data. Although it may...Since most ELNs, unlike data repositories, are designed to support confidentiality and limit permissions...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...utilizing chemogenetics (Designer Receptors Exclusively Activated By Designer Drugs, DREADDs) and optogenetics...examining the use of DREADDs (Designer Receptors Exclusively Activated By Designer Drugs) to determine the ...

  5. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...many MoClo kits are designed with specific model organisms in mind, others are designed for flexibility. .... phaffii, then the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al... the Multi-Kingdom (MK) Golden Gate system was designed to work in fungi, bacteria, protists, plants, ...

  6. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab - Control neuronal activity with a magnetic field. Listen...Listen to Our Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's...

  7. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    .... “When PhD students graduate there has to be champagne, that's a given.” Furthermore, anyone leaving ...fellowships, jobs, or paper submissions with dinner and champagne in the lab. The Church Lab celebrates successful...toasts a newly minted PhD with food, cake, and champagne. What does your lab do for fun as a group? The...

  8. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ... them. For the application we were looking at, magnetic isolation of circulating tumor cells, we found...may have an impact on this challenge either for magnetic capture or for capture using microfluidic devices...peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Fierer et al (Proc Natl Acad...

  9. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ... Roth BL (2015) DREADDs (Designer Receptors Exclusively Activated by Designer Drugs): Chemogenetic Tools...less invasive and more flexible as it relies on designer ligands that can be simply introduced by injection...

  10. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...why each is important. Our “Plasmids 101” series designed to educate all levels of scientists and plasmid...basics and spend more time developing cleverly designed experiments and innovative solutions necessary...utilized in the lab are usually artificial and designed to introduce foreign DNA into another cell. Minimally...

  11. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ..., IDT scientists Mollie Schubert and Shuqi Yan designed experiments to test nickase preferences using ...between the nicks? To find out, Schubert and Yan designed a PAM-out nickase experiment to insert an EcoRI...that are further away from the target site, they designed a D10A nickase strategy (46 nt nick distance, ...

  12. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...verification experiments that can be partially designed using the Snapgene desktop software. If a plasmid...verify the sequence within that region. We have designed many primers for Sanger sequencing at Addgene....function, we can detect all of the primers we have designed that will anneal to the plasmid sequence. We can...

  13. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...multiple fragments of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA...purification steps and end-repair steps with well-designed fragment overlap sections, while still creating... individual fragments containing mutations and aligned via regions of homology. With NEBuilder HiFi DNA...

  14. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...or performance review. You can’t delegate tasks assigned to you and you must usually take on all tasks ... be able to communicate these well to the task assignee and this is a recipe for bad outcomes. Clearly...little, but be careful not to go too far. Is the assignee interested in this task? Don’t give out tasks ...

  15. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein...brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will work better with shorter...

  16. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ... Addgene guest blogger post about colony PCR, I signed up immediately. I had used colony PCR before and...this method. I enjoyed writing the first post so I signed up to write a second. While working on this post...use Twitter as a scientist. While this post is designed with beginners in mind, there’s some great reminders...

  17. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs... at 3 days post fertilization. Since Ma et al. designed a repair template with two synonymous mutations...thus quieting some fears about the creation of “designer babies.” The differences in editing between iPS...

  18. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...machine-learning model to help them construct “designer” channelrhodopsins with desired characteristics...experiments. The sequences in this library were designed using structural information about parental channelrhodopsins...existing channelrhodopsins CoChR and/or ChR2(H134R). Designer channelrhodopsins paired with a systemic AAV allow...

  19. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...ability to correctly assemble a lacI/lacZ cassette (designed by NEB for use in optimization of assembly systems...methodology. To further push the test system, we redesigned for 12 and 24 fragments.  This redesign was guided...fragment versions of the lacI/lacZ cassette were designed and synthesized. In conjunction with BsaI-HFv2...

  20. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...We should note that these vectors are primarily designed for bicistronic expression; however, many could...the table above (and their related plasmids) are designed to have one or more of the genes replaced.  Additionally...save on your desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid...
Showing: 21 - 40 of 293 results