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We narrowed to 273 results for: phy

Showing: 21 - 40 of 273 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...and on self-propagation by reproduction. Both physiological processes are controlled by deliberate and compulsory...receptors (NRs), their coregulators, and their physiological endocrine ligands. Nuclear receptors and their...circadian regulation, as well as in important physiological states such as adipogenesis and macrophage activation...Mangelsdorf, D. J. (2001). Nuclear receptors and lipid physiology: opening the X-files. Science 294, 1866-1870....

  2. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...transduction of cells." American Journal of Physiology-Cell Physiology 291.2 (2006): C386-C392. PubMed PMID:...Different titering methods can reveal differences in physical versus functional titer. If you have low titers... virus adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. PubMed PMID: ...

  3. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...dicots Self-labeling tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO ... SNAP! Self-labeling tags for single-molecule biophysics By Mike Lacy In order to study the conformational...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...receptors (GPCRs) are critical components of many physiological pathways and represent the largest family of...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...to exploration and collaboration. Andrew is a physicist with a background in optics and a track record... readily admit they don’t fully understand the physical mechanism, though they have collected a variety...of fields, like quantum chemistry and protein biophysics, as well as inventors and biomedical engineers...Gating Mechanism for Magnetogenetic Ion Channels. Biophysical Journal, 116(3), 454–468. https://doi.org/10.1016...

  5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...even when larger vector genomes are packaged, the physical size of the genomes is still ~4.7kb. But despite...-dystrophin gene to a mouse model of muscular dystrophy. While mini-dystrophin expression levels were ...overlapping split vector had an improvement in muscle physiological performance.  2. Trans-splicing Overview: For...-dystrophin gene to a mouse model of muscular dystrophy. Three different splice site donor and acceptor...

  6. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...laboratory, then FRET experiments are a viable biophysical path to answers. What is FRET and what's it...Fluorescence resonance energy transfer (FRET) is a biophysical interaction between two fluorophores. When the... In fact, FRET can be used to derive a precise physical distance between the donor/acceptor fluorophores...and purification is a snap using thin layer chromatography (Fessenden, 2009). However, these reagents ...

  7. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It’s important...shown. Top: The follower network of life (green), physical (blue) and social (orange)  scientists largely...disciplines: social (left), life (center), and physical (right). How do you use Twitter as a scientist...

  8. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    Published
    July 18, 2017, 12:41 p.m.
    ...agricultural plant. Her current research focuses on endophytes, bacteria, and fungi that reside within living...antimicrobial activities (1). She has developed a fast endophyte screening system and plans to employ the light‐inducible...References 1. Martinez-Klimova, Elena et al. “Endophytes as sources of antibiotic” Biochemical Pharmacology...

  9. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...behavior, or the animals being monitored were physically hooked up to invasive, cumbersome systems during...optogenetic purposes, measure temperature, study electrophysiology, and deliver small molecules.   Fun fact:...fibers as previously described, then measured electrophysiological potential afterwards. This measurement trended...

  10. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...complexity of translational control in cellular physiology. Ribosome profiling Ribosome profiling, a biochemical...translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating RNA...studying translation in various cell types and physiological states. References 1. Ingolia, Nicholas T.,...

  11. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...Additionally, the Phylogeny tool allows users to browse entries by the current phylogenetic nomenclature and... CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics, and Structural Biology. https://doi.org/10.32942...

  12. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...lines appearing in American Journal of Physiology-Cell Physiology over the past decade. For more practical...might suggest underlying sex-based influences on physiologic or pathologic processes. Much progress has been...

  13. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...PMID: 27579989 DECEMBER 2016 Restoring yeast prototrophy with plasmids Though auxotrophic markers are ...auxotrophic backgrounds can lead to disparate physiological effects and complicate any metabolic study. ...lab has created a unique tool with their Yeast Prototrophy Kit, a set of plasmids designed to complement... and besides being used for restoring strain prototrophy, can also aid in designing self-establishing ... pooled library Parasites from the apicomplexan phylum cause severe human and livestock diseases such ...

  14. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...but are not limited to, ones targeting muscular dystrophy, cardiovascular disease, inflammatory diseases...editing frequency isn’t high enough to make a physiological difference, then the treatment will not work...blog CRISPR Antimicrobials Treating Muscular Dystrophy with CRISPR CRISPR 101: Drug Discovery and CRISPR...

  16. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...Ample distribution channels both digitally and physically. Easy to incorporate visuals if needed. Traditional...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...levels and interaction. Can bring in things to be physically interacted with. Public speaking is a common ...

  17. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...expressed can cause facioscapulohumeral muscular dystrophy (FSHD), a disease for which there is currently...immunoprecipitation (enChIP) using CRISPR." Biochemical and biophysical research communications 439.1 (2013): 132-136... Fujita, Toshitsugu, et al. "Identification of physical interactions between genomic regions by enCh IP‐Seq...

  18. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...be expanded to ~4-10x linearly! This expansion physically separates molecules while maintaining their relative...   Figure 1: The ExM process allows you to physically magnify a sample so you don’t have to optically...ambient temperatures, incubation times, and the physical properties of the sample itself. For example, ...

  19. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...diseases such as hemophilia, muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic...life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV gene therapy started in the mid-90's with...

  20. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ... Streptococcus pyogenes (Sp) 3' NGG Staphylococcus aureus (Sa) NGRRT or NGRRN Neisseria ... useful than SpCas9. For example, Cas9 from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller...Zhang F (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org...
Showing: 21 - 40 of 273 results