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We narrowed to 413 results for: vent

Showing: 21 - 40 of 413 results

  1. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...while with conventional gene targeting, the cost may be $50,000. Finally, compared to conventional methods...easy. In the 1980s, gene targeting technology was invented to introduce specific changes into the mouse genome...CRISPR mouse experiment Everything changed with the advent of CRISPR in 2013 (Cong et al., 2013)! Haoyi got...mouse models using CRISPR is easier than more conventional methods. Time and money savings come from the...reagents directly into fertilized mouse eggs, circumventing the need for enrichment and selection offered...of CRISPR mouse editing when compared to more conventional methods. First, one can work with almost any...any strains of mice, as compared with conventional gene targeting, which is limited to a few strains, including...

  2. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...scientists to not only easily identify base editing events in real-time but also enrich for base-edited cells...single base edits (St. Martin et al., 2018). To circumvent sequencing and the need for a DSB in their previous...TLDR: The eGFP reporters from the Harris Lab circumvents the need for DSBs in monitoring on-target DNA...to a ‘TAC’ or ‘TAT’ after a C-to-T base editing event. This edit changes the histidine to a tyrosine generating...emission spectra. Thus a successful base editing event would result in GFP fluorescence that can be visualized...variant that converts to GFP after a base editing event. GFP positive cells can be sorted and have a significantly...reporter that detects and enriches for base editing events in vivo without relying solely on GFP (Katti et...

  3. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...organize events that bring different voices together to talk about genetics. In addition to events that we...stream of events mean that there is never a dull day in the office. And in the run-up to a major event, such...benefiting from the fruits of scientific research, and prevent technological advances from exacerbating existing...experiences in a variety of other settings, including “conventional” academic venues such as scientific, educational...industry conferences, as well as schools, community events, churches or mosques. On occasion, we also get ...getting experience in either giving or running these events will be important both in preparing you for future...

  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... This means they do not reliably detect editing events that occur in less that 5% of the population (Fu...genome predicted to contain off-target cleavage events. Unbiased techniques search the whole genome for...irreproducible data in a research setting, but these events could have disastrous health effects in the clinic...a result, only sequencing sites predicted by conventional algorithms may not provide a full picture of...a more comprehensive list of off-target editing events. Two of the most common cell-based methods are ...should give a broad picture of all possible editing events in the genome; from there, amplicon sequencing ...necessary for every CRISPR experiment. Off-target events due to >3 bp mismatches or that are sequence-independent...

  5. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...mutations and gene editing Targeted genome editing events fall into two general classes: knock-in and knock-out...frameshift mutation. The majority of cellular repair events will generate these types of mutations, making ...considerations for designing donor DNA for successful HDR events. CRISPR cut site location Position your CRISPR...disrupting mutations in donor DNA   Promoting HDR events over other edits Like I said earlier, HDR is not...shown to be efficacious for increasing knock-in events (Yang et al., Int J Mol Sci). Adding these inhibitors... to exist as a repair template. To maximize HDR events, ensure cells are actively cycling through the ...Charpentier et al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions...

  6. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... any preventive measure designed to help keep you from getting sick can be helpful in preventing the spread... on preventing the spread of antibiotic resistant bacteria, check out this resource on preventing antibiotic...because ‘antibiotic’ merely means that the drug prevents the growth of a living organism. As for multi-...standpoint, we can all work together to try and prevent outbreaks of drug resistant bacteria within communities...standards. Handwashing is an essential component of preventing infection, as is cleaning and disinfecting frequently...

  7. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...have shown recently that desired genome-editing events by HDR can be generated more efficiently by (1)...1) improving editing accuracy by preventing re-editing and (2) optimizing “cut-to-mutation distance”. ...as APP and PSEN1, in most cases the intended HDR events were corrupted by unwanted indels on the same allele... was that these “blocking mutations” mutations prevent CRISPR from re-cutting the target sequence once... and found that for generating a homozygous HDR event with optimal efficiency a guide RNA targeting a ...desired mutation should be used. For a heterozygous event, the cut should ideally be around 5 to 20 bp away...away from the cut site. Lastly, if a heterozygous event is desired but only guide RNAs that target very ...

  8. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...important binding events take place during the course of infection. These binding events can be impaired...subcultured at densities that are low enough to prevent confluency but high enough to promote cell growth...the negatively charged cell with the virus. To circumvent this problem, transductions typically employ ...Incidence, sources, effects, detection, elimination, prevention.” Cytotechnology 39.2 (2002). 75-90. Pubmed PMID...of infection (MOI) for predicting gene transfer events.” Genetic Vaccines and Therapy 2.1 (2004) Pubmed...

  9. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...recombination to generate additional recombination events, and the synthesis of alternative loxP sites to... removes one of the other loxP site variants, preventing further recombination. Brainbow-1.1 scales up...to four fluorescent proteins, with recombination events specified by three loxP variants, but the principles...that transient Cre recombination is required to prevent constant inversion of the construct. Sanes and...combinatorial effect of multiple, independent recombination events leads to a rainbow of colors. With three copies...their protein sequences are highly homologous, preventing design of antibodies specific to each fluorophore...

  10. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...tissue damage, have excessive bone growth until eventually the patient loses mobility. Attempts to remove...body could initiate very painful FOP flare ups. Eventually, without a cure, AJ will begin losing mobility... with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic... Super Hero 5K on October 23rd. This is a great event at which people dress up like superheroes or other... to AJ.   PS: The superhero race is the perfect event for supporting AJ, who is a big superhero fan. This...

  11. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...In this study, the labs found that anti-CRISPR prevents Cas9 activity in HEK293 by reducing editing from... Acr samples. Acr proteins can also be used to prevent dCas9 tethering to DNA. Since these early anti-... AcrIIC2 interactions with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex ...binds to the HNH endonuclease domain of Cas9 and prevents target DNA cleavage (Harrington et al., 2017)....vectors. Having a way to shut off CRISPR-Cas systems prevents the off-target effects and cytotoxicity that result...

  12. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...can be used to induce site-specific recombination events, and that the orientation of the flanking loxP ...target sites to induce site specific recombination events: Cre recombinase binds loxP sites, while FLP binds...fragment (Figure 1, middle). A second recombination event then excises the DNA between the identical loxP ... the DNA fragment, any additional recombination events are impossible even in the presence of Cre recombinase...and GeneR fragment. Then the second recombination event would excise the reporter from the cassette, allowing...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications...applications. But how does one invent a tool for applications that don’t even exist yet? Andrew York, Maria... After a few more rounds of mutagenesis, they eventually found a version of AsLOV2 with five mutations...applications are still yet to be demonstrated (or invented), its behavior has already been successfully reproduced... chemistry and protein biophysics, as well as inventors and biomedical engineers, join efforts to explore...

  14. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...organizing events. I contacted local professional organizations and nonprofits. I volunteered at events like...Science Outreach, or CINSO. We organize fun science events for adults and professional development opportunities... development including workshops and networking events. I took advantage of as many of these resources...graduate school resources daily to promote CINSO events and recruit speakers and participants for our professional...

  15. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...were to escape the laboratory, the drive could eventually spread through the entire wild population with...create an RNA-guided gene drive. In principle, this event could happen even if there isn't any homology available...undo the genetic changes driven by the earlier intervention2. While an initial reversal drive cannot restore...in building candidate gene drives intended for eventual release should consider building an appropriate...publications. Thank you to our guest blogger! An inventor of technologies that harness evolution, Kevin ...

  16. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...usually have a good event-planning base and will know how to reserve space, time events, and network for ...general meeting and a means to request funding for events, consider reaching out to it. PTAs have more flexibility...volunteers. Speaking of volunteers, you can’t run an event like this without them! If you live in a school ...curriculum coordinator with minimal luck, but eventually the 8th grade science teachers at our school ...

  17. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...these sequences. To prevent re-cutting and the resulting mutations after an HDR event, ensure the gRNA or... other on the non-crossing strand), a crossover event will occur; however, if both HJs are resolved in...the same manner, this results in a non-crossover event.  Synthesis-dependent strand-annealing (SDSA) pathway...mechanism which exclusively yields non-crossover events. This means all newly synthesized sequences are...

  18. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ... an event, and assigns each event to a channel number based on intensity. Higher intensity events are ...assay a user will compare the shifting intensity of events. Since intensity positively correlates with expression...preparation, staining procedures, and controls. To prevent instrument clogs, ensure that samples are single...

  19. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...communicators. In this chapter we learn about some of their events - science seminars, science cafes, and the SITN...discussed below). Chpater 3: SITN Popularity and Events - Amy discusses some of the programs SITN runs ...short-form blog posts help readers think through current events. Chapter 6: The SITN Editorial Process -Vini discusses...the Pint - We learn why a "Science by the Pint" event is the best way to get a Science Communication group...

  20. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...CRISPR construct needs to be segregated away to prevent continued production of new alleles or off-target...it. I have used it for selecting transformation events and am currently planning to use it to simplify...expression can vary from different transformation events likely due to insertional effects in the genome... generation when CRISPR needs to be removed to prevent further editing, then green offspring can be selected...
Showing: 21 - 40 of 413 results