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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 580 results for: RON

Showing: 381 - 400 of 580 results

  1. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...Joanne's Reddit AMA 6. Practice, practice, practice in front of a real audience Good, even great, speaking skills...help you avoid giving only yes or no answers in front of a crowd. Having answer slides ready after your...

  2. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...restore the dystrophin reading frame. gRNAs bind in intronic regions, so low-frequency indels will not cause...exonic regions comes with much more risk than the intronic editing used in the exon skipping approaches here...

  3. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...excessive costs in your lab. That in itself is a strong negotiating tactic, and puts you ahead of most ... create productive and manageable laboratory environments. His business skills enhance his passion for...

  4. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...mediated dsDNA breaks: following a cut, the error-prone nature of non-homologous end joining (NHEJ) often...optimized sequence will do little if it is in the wrong place, but because the target window is more-narrow...

  5. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...reputation? If you are choosing a training lab, I strongly suggest you complete a work rotation in the lab...and non-academic career paths. I can’t say this strongly enough - don’t choose a mentor that sees you only...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...brought together and microinjected into either the pronucleus or the cytoplasm of fertilized mouse eggs. Or...remember that you are working with RNAs, which are prone to degradation by the omnipresent RNase. You may...

  8. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering and everything...policy training programs and fellowships have a strong focus on early career researchers who have decided...

  9. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...extreme sequence divergence that requires a multi-pronged analysis to sort effectively. The current classification... functions within Class 1 CRISPR-CAS systems. Frontiers in Microbiology, 12. https://doi.org/10.3389/fmicb...

  10. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications, vol. 2, no. 2, Elsevier...10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR...

  11. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...the labeling kinetics or the fluorophore’s microenvironment to enhance brightness, stability, or other....1021/ar200099f  Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H., & Johnsson, K. (2003...

  12. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...found that the mutations that emerged depended strongly on the target edit (Doman et al., 2023). Therefore...editors with RT domains derived from the E. coli Ec48 retron RT and the S. pombe Tf1 retrotransposon RT, respectively...

  16. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers...

  18. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...are used in the lab? Check out our “Rabies and Neuronal Tracing” blog post! You can also browse tools ...

  19. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ... 2: Magneto 2.0 form Ali Guler's Lab - Control neuronal activity with a magnetic field. Listen to Our ...

  20. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...regulatory element, which targets it to VIP cortical interneurons. Take a look to see if these new preps might...
Showing: 381 - 400 of 580 results