We narrowed to 598 results for: abo.1

... remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is maintained in...follow him on twitter: @joachimgoedhart.   References 1. Zacharias, David A., et al. "Partitioning of lipid-modified...deleting residues. Only about 10 amino acids can be deleted from the C-terminus and about 5 from the N-terminus...cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel...based on cofactor binding proteins as described above. The smallest tags are peptides that are engineered...

...at a low angle (FSC) or large angle (SSC) (Figure 1). FSC values depend on the cell's size, while SSC ... or ask experienced colleagues.   Figure 1: The laser pulse illuminates the cell and is scattered...lymphocytes in a mouse tumor sample You made it! So much about the theoretical background. Let’s dive into the ...often choose a looser gate here. I am not worried about including irrelevant events in my analysis, as these...population, acquired from the previous gating pipeline, about 53% of my cells are dendritic cells and ~13% are...ve covered in this blog post. First, you learned about the types of scattering that occur when a cell is...

...improved sensors for these and other targets. Figure 1: MTRIA biosensors are generated by inserting a circularly-permuted...fluorescent biosensors. Nat Commun. 2022 May 25;13(1):2919. doi: 10.1038/s41467-022-30685-x. Feel the ...screen (Koveal et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable...valuable tool to study cellular physiology and metabolism under various conditions. LiLac has improved response...going viral! In addition to the viral preps noted above for iGluSnFR3 and JEDI-2P, several biosensors already...innovation next year! Did we miss any? Let us know about your favorite new biosensor plasmids in the comments...

...five methods for extracting DNA without a kit too. 1. Early Career Researcher Toolbox: Free Online Molecular... highlighted reagent repositories and other collaborative efforts in the scientific community to speed...store my plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post....dominates DNA quantification discussions, but what about the other methods to quantify DNA? Here we cover...Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids in one cell, it might ...

...downstream experiments. You only successfully generate 1 null clone and proceed with it. In this case, the ...there are 10 putative off-target locations you can 1) determine if editing at those sites would confound...is an off-target effect. Should I be concerned about off-target effects? Depending on your experimental...this could be a huge concern, or nothing to worry about all. Let’s consider several scenarios below for ...Predicting off-target events As mentioned in the above section, there are gRNA softwares available which...diligence to ensure they are indeed a non-issue. Above we mentioned how to predict off-target frequency...

...2013 edition. 10 new developments in open science 1)      In February, the Office of Science and Technology...study probably doesn’t say as much about open access as it does about the state of peer review. 8)     ...University Open Data Access (YODA) Project, “a collaborative endeavor guided by aspirations to promote open...

...together along with the Gibson assembly master mix for 1 hour, and then transform into competent cells. The...you should consider giving it a try! References: 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison...the annealed and filled-in gaps The great part about this mix of enzymes is that they can all work at...the standard Gibson assembly techniques described above, this resulted in the direct and seamless cloning...Blog Browse All of Our Plasmids 101 Posts Read About Other Cloning Techniques Additional Resources on...

...Cloning grade DNA Transfection grade DNA Figure 1: Transformation introduces transformation grade plasmids... cloning Read our Plasmids 101 blog posts Learn about the differences between transformation, transduction...

... successful after their training.   Figure 1: Professional Development Hub Workshop Attendees and...March 4th from 2:30-4:00 pm ET.  You can learn more about the report's recommendations and join with us to...

... with DNA sequencing troubleshooting and analysis 1. You can use any of the following programs to view...and troubleshooting your DNA sequencing results Above (top right corner of this post) is an example of...

...infections, cancer, and other diseases. References 1. Harrington, Lucas B., et al. "Programmed DNA destruction...Additional resources on the Addgene Blog Learn more about DETECTR Browse all of the CRISPR blog posts Download...

...making it easier for us to do our work.” References 1. Athanasios Lampropoulos et al. "GreenGate - A Novel...protein,” he says. “There is nothing you can do about it but be gentle. If it no longer works, just trash...

...constructs to the kit in the next two years. References 1. Martz, Colin A., et al. "Systematic identification...he and his colleagues at Duke University, in collaboration with “cloning guru” Kathleen Ottina and David...it cloning by mail,” Wood says, and it took them about six months from start to finish. They cloned all...

...Science in the News Chapter 1: Introduction - Vini and Amy tell us a little about themselves. Chapter 2: What...To learn more about the STIN's podcast, email [email protected]    For more info about starting your...evens where scientists can grab a beer and chat about their work at a local bar. It doesn't have to be...science communicators. In this chapter we learn about some of their events - science seminars, science...the lab and working with SITN. She points to collaboration and delegating as keys achieving success. Chapter...your own SciComm organization and to learn about some of SITN's other initiatives, email [email protected]...

...products by their environmental impact on a scale of 1-10: the lower the score, the lower the environmental...well-integrated into our lab lives. Read on to learn about some creative approaches to lab sustainability, ...for rerouting waste from the landfill, but what about all the manufacturing steps that happen before you...ACT label, which is an eco-nutrition label for laboratory products that emphasizes Accountability, Consistency...away your FACS machine after a single use. So how about washing and reusing pipette tips in a high-throughput...Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the...

...Protocols Publication describing them here. References 1.Ran, F. Ann, et al. "Genome engineering using the ...the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common...

...deletions, and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. 5. Five Popular...

...relations, advocacy, and writing legislation (see Figure 1 for career map). The beautiful part of science policy...policy as a profession. Figure 1: Science policy can lead you to many paths! For a more details,...learn more about their career trajectory. If there’s one thing everyone loves, it’s talking about themselves...learn more about the field.  Read the CCST Science Policy Career Guide to learn more about science policy...Administration, I could never get one straight answer about what science policy even is! In this article, I ... the #scipol hashtag. Often there will be posts about virtual meet ups and happy hours that you can attend...solving one problem. It often requires thinking about a problem in a new way through the integration of...

...guaranteed. To learn more about this process check out our Mouse Modeling blog posts: Part 1: Genetically Engineered...Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning... Delivery Methods Learn about Mouse Work on Exercise and Depression Read About Brainbow Resources on ...by CRISPR. The sequence started clean and then, about 100 bps into the run, it got “dirty”, with multiple...makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease...by Southern blot. From the efficiency discussed above extends 4 major advantages of CRISPR mouse editing...targeting. It also costs less. With CRISPR, it may cost about $5,000 to generate founder mice, while with conventional...

...furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse of...cellular analysis products at Promega.     References 1. Hall, M.P., et al. (2012) Engineered luciferase reporter...Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent Protein...heme-containing proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often... emits light at a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed...be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...