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We narrowed to 584 results for: abo.1

Showing: 381 - 400 of 584 results

  1. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...making it easier for us to do our work.” References 1. Athanasios Lampropoulos et al. "GreenGate - A Novel...protein,” he says. “There is nothing you can do about it but be gentle. If it no longer works, just trash...

  2. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...constructs to the kit in the next two years. References 1. Martz, Colin A., et al. "Systematic identification...he and his colleagues at Duke University, in collaboration with “cloning guru” Kathleen Ottina and David...it cloning by mail,” Wood says, and it took them about six months from start to finish. They cloned all...

  3. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...Science in the News Chapter 1: Introduction - Vini and Amy tell us a little about themselves. Chapter 2: What...To learn more about the STIN's podcast, email [email protected]    For more info about starting your...evens where scientists can grab a beer and chat about their work at a local bar. It doesn't have to be...science communicators. In this chapter we learn about some of their events - science seminars, science...the lab and working with SITN. She points to collaboration and delegating as keys achieving success. Chapter...your own SciComm organization and to learn about some of SITN's other initiatives, email [email protected]...

  4. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...products by their environmental impact on a scale of 1-10: the lower the score, the lower the environmental...well-integrated into our lab lives. Read on to learn about some creative approaches to lab sustainability, ...for rerouting waste from the landfill, but what about all the manufacturing steps that happen before you...ACT label, which is an eco-nutrition label for laboratory products that emphasizes Accountability, Consistency...away your FACS machine after a single use. So how about washing and reusing pipette tips in a high-throughput...Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the...

  5. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...Protocols Publication describing them here. References 1.Ran, F. Ann, et al. "Genome engineering using the ...the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common...

  6. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...deletions, and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. 5. Five Popular...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...or one pyrimidine base to the other. Figure 1: Base transition editors catalyze purine-to-purine...SpCas9 base editors with editing windows as small as 1-2 nucleotides. To reduce off-target effects associated...engineering and protein delivery. Nature Communications, 8(1), 15790. https://doi.org/10.1038/ncomms15790 Richter...Homology-Directed Repair Learn about base editing for plants Read about four base editor reporters Additional... The BE3 system increased editing frequency to above 30% for a variety of targets in human cell lines... Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine base editor with improved...cell therapy applications. Dual base editors What about combining the function of both editors into one?...

  8. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...relations, advocacy, and writing legislation (see Figure 1 for career map). The beautiful part of science policy...policy as a profession. Figure 1: Science policy can lead you to many paths! For a more details,...learn more about their career trajectory. If there’s one thing everyone loves, it’s talking about themselves...learn more about the field.  Read the CCST Science Policy Career Guide to learn more about science policy...Administration, I could never get one straight answer about what science policy even is! In this article, I ... the #scipol hashtag. Often there will be posts about virtual meet ups and happy hours that you can attend...solving one problem. It often requires thinking about a problem in a new way through the integration of...

  9. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...guaranteed. To learn more about this process check out our Mouse Modeling blog posts: Part 1: Genetically Engineered...Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning... Delivery Methods Learn about Mouse Work on Exercise and Depression Read About Brainbow Resources on ...by CRISPR. The sequence started clean and then, about 100 bps into the run, it got “dirty”, with multiple...makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease...by Southern blot. From the efficiency discussed above extends 4 major advantages of CRISPR mouse editing...targeting. It also costs less. With CRISPR, it may cost about $5,000 to generate founder mice, while with conventional...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse of...cellular analysis products at Promega.     References 1. Hall, M.P., et al. (2012) Engineered luciferase reporter...Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent Protein...heme-containing proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often... emits light at a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed...be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...synapses and deep-brain fiber photometry.  Figure 1: Characterization of iGluSnFR4 in mice. A) Scheme ...CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-1 cells (colorectal cancer). Image courtesy of Brianna...insert your genetic cargo of choice. After hearing about researchers' favorite features and limitations of...development and cancer. But there is still much to learn about their myriad functions and therapeutic potential...created a very quick and efficient way to learn more about gene regulation. Find the TurboCas plasmid here!...Initiative Armamentarium Collection, a major collaborative effort to develop molecular tools for studying...

  12. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...strategies in the comments section!   References 1. Yearly Planning Meetings: Individualized Development... development at the center of her lab (pictured above). DePace holds yearly planning meetings with each... their work rather than overwhelmed or confused about what’s coming next. Goal setting and career planning...and their next steps. Addgene would love to hear about the steps taken in your lab to promote personal ...

  14. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...organized and boost your productivity in 2014. Tip 1: Use color coding, labels, and post-its to make finding...experiments before the next R01 is due. But what about organizing yourself and your lab? Organizing your...

  16. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...and drawbacks of four split AAV vector approaches. 1. Overlapping  Overview: The overlapping strategy has...Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140 Frischmeyer...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...

  17. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...P2A-PuroR for lentiviral expression. Figure 1: dCas9-p300 adds H3K27ac marks to histones in promoter...lentiviral transduction. Lysine-specific Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited ...modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although...setting up your experimental system. Let us know about your experience with these constructs in the comments...

  18. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...to western blotting  In a western, proteins are: (1) separated by size, (2) transferred to a membrane,...  (3) detected using antibodies.   Figure 1: Overview of the Western blot process. The sample,... the thickness of the band provides information about the relative abundance of the protein in the sample...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...

  19. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ... towards understanding this disease. Figure 1: Scientists are continuing to do essential research...blog Read about COVID-19 research on the blog Visit our guide for external collaborations Resources ...scientific sharing. Already we’ve seen articles about COVID-19 on preprint servers and in journals, and...vary, everyone we spoke with sees sharing and collaboration as the best way that the scientific community...useful resources during this time. Facilitate collaborations within the scientific community The team behind... Canada is a database that provides information about reagents, funding and volunteer opportunities, protocols...CCRP2) at Rutgers University not only supports collaborations at Rutgers, but also lists data and experimental...

  20. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ... if the name was the correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  ... of Bioinformatics reached out to us to tell us about a resource called Cellosaurus, a growing cell line...all the information in the resource.  This is a labor-intensive project; scripts can be used in some cases...preferences to help you identify proximal lines you care about. If you have cell lines without a certified origin... Or finding errors in the available information about a cell line you’ve used? Submit your manuscript ...distributor. A success all around! If you’re excited about this resource and want to learn more, check out ...
Showing: 381 - 400 of 584 results