We narrowed to 468 results for: SEC

1. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...associated with the library and reading their methods sections to see what worked.  Ready, optimize, transduce...

2. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ...subsequently degraded at a rate of ~1,250 cuts per second. A quantifiable fluorescent signal designates the...

3. Plasmids 101: Plasmid Incompatibility

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   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...repressed and replication is downregulated. In the second model, termed the “handcuff” model, iterons of ...

4. 5 Reasons to Use Reddit for Science Communication

   Type

   Blog Post

   Published

   Oct. 20, 2016, 2:30 p.m.

   ...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...

5. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...successful lentiviral infection, happy virus is the second. When harvesting lentivirus, some labs prefer to...

6. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...inhibition of hippocampal neurons. Initial monitoring Second, this may sound rudimentary, but before you do ...

7. Plasmids 101: Control Plasmids

   Type

   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...more below, but the papers cited in the reference section provide more in depth information. Technical replicates...

8. Data Freedom: The Expansion of Data Sharing in Research Publications

   Type

   Blog Post

   Published

   Aug. 5, 2014, 6:51 p.m.

   ...intimidated by having to contact more senior colleagues. Secondly, science is highly technical and this has a natural...

9. Hot Plasmids: Summer 2024

   Type

   Blog Post

   Published

   Aug. 27, 2024, 1:15 p.m.

   ...here!   Figure 5: Free-floating rat brain sections stained with Anti-GFAP. The staining patterns ...

10. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ...fluorescent stain, you might be wondering which second measurement to choose for graphically displaying...

11. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    Published

    March 28, 2024, 1:15 p.m.

    ...that bind the same ligand, making it difficult to dissect the function of one integrin unless others are ...

12. Hot Biosensors 2022: Year-End Roundup

    Type

    Blog Post

    Published

    Dec. 6, 2022, 2:15 p.m.

    ...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

13. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    Published

    Nov. 3, 2022, 12:15 p.m.

    ...helpful to know how many copies you are looking for. Secondly, categorize your edit – is it any frameshift mutation...

14. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type

    Blog Post

    Published

    July 10, 2018, 1:08 p.m.

    ...assigning team members to certain tasks, etc. How is security and user access to data defined within your lab...

15. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    Published

    March 5, 2019, 1:08 p.m.

    ...dBrainbow includes small epitopes fused to each XFP. Secondary antibodies that target these epitopes and fluoresce...

16. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    Published

    June 3, 2021, 1:15 p.m.

    ...to produce scFvs in mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid...

17. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    Published

    May 20, 2021, 1:15 p.m.

    ...their leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over...

18. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    Published

    Sept. 28, 2023, 1:15 p.m.

    ...Figure 3).  The Hehnly Lab used such a strategy to dissect the roles of three Rab proteins during a key stage...

19. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...to find the right one Step 2 – Edit the cells a second time using a repair template that corrects the ...

20. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger Krissy...