We narrowed to 478 results for: SEC

...to find the right one Step 2 – Edit the cells a second time using a repair template that corrects the ...

...to produce scFvs in mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid...

...their leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over...

...assigning team members to certain tasks, etc. How is security and user access to data defined within your lab...

...dBrainbow includes small epitopes fused to each XFP. Secondary antibodies that target these epitopes and fluoresce...

...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

...helpful to know how many copies you are looking for. Secondly, categorize your edit – is it any frameshift mutation...

...favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger Krissy...

...participated as a member of the Career Paths Panel on the second day of the symposium and live tweeted throughout...

...part of the spectrum: PinkyCaMP from the Olivia Masseck Lab (Fink & Imai et al., 2024) and FRCaMPi from...

...Predicting off-target events As mentioned in the above section, there are gRNA softwares available which score...

...may your needed volume (de Backer et al., 2010).  Second, high efficiency transduction coupled with a strong...

... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

...be more challenging for sequences with complex secondary structure. Today, multiple companies offer long...

...Therefore, in addition to their utility in the health sector, phages represent a reservoir of carbohydrate binding...

...for which we have germline competent ESC lines. Second, the process is much quicker. It takes 3 months...

...and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid, you could use...

...through Addgene’s viral service and the methods sections of scientific articles (gasp). To my delight, ...

... in your system. It is very rare for a methods section to include all of the variables that need to be...

...active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells. piggyBac...