We narrowed to 468 results for: SEC

1. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   Published

   Nov. 25, 2015, 4 p.m.

   ...participated as a member of the Career Paths Panel on the second day of the symposium and live tweeted throughout...

2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   Published

   April 5, 2018, 12:42 p.m.

   ...be more challenging for sequences with complex secondary structure. Today, multiple companies offer long...

3. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   Published

   Feb. 8, 2018, 12:17 p.m.

   ...fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins to make better...

4. CRISPR 101: Off-Target Effects

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   Blog Post

   Published

   Feb. 8, 2024, 2:15 p.m.

   ...Predicting off-target events As mentioned in the above section, there are gRNA softwares available which score...

5. Viral Vectors 101: AAV Variables That Matter

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   Blog Post

   Published

   June 1, 2023, 1:15 p.m.

   ...may your needed volume (de Backer et al., 2010).  Second, high efficiency transduction coupled with a strong...

6. Plasmids 101: Dimers and Multimers

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   Blog Post

   Published

   Jan. 10, 2023, 2:15 p.m.

   ... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

7. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   Published

   Sept. 29, 2016, 2:30 p.m.

   ...Therefore, in addition to their utility in the health sector, phages represent a reservoir of carbohydrate binding...

8. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   Published

   July 12, 2016, 2:30 p.m.

   ...for which we have germline competent ESC lines. Second, the process is much quicker. It takes 3 months...

9. PiggyBac-ing Through the Genome Editing Field

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   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid, you could use...

10. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...from Pyrococcus furiosus." Acta Crystallographica Section F: Structural Biology and Crystallization Communications...

11. Finding Your Science Policy Path

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    Blog Post

    Published

    Sept. 22, 2020, 1:15 p.m.

    ...also happen at the state and local level. The big secret about science policy that I didn’t learn until ...

12. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    Published

    April 16, 2024, 1:15 p.m.

    ...Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer mitochondrial membrane...

13. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ...through Addgene’s viral service and the methods sections of scientific articles (gasp). To my delight, ...

14. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ... in your system. It is very rare for a methods section to include all of the variables that need to be...

15. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    Published

    July 6, 2021, 1:28 p.m.

    ...active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells. piggyBac...

16. A Guide to Getting Started in Undergrad Research

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    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ... was interested in microbes until the end of my second year! Conclusion and other pieces of advice Give...

17. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control...

18. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ... flow cytometry. Let's briefly go through each section of this article again. In the beginning, we talked...

19. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ...of CRISPR overall. Type I systems degrade large sections of DNA thanks to the dual action power of Cas3...

20. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    Published

    Oct. 24, 2017, 1:54 p.m.

    ...fact is that we are citizens first and scientists second and that it is our duty to tell our science stories...