We narrowed to 740 results for: NSI

... they flank the gene of interest. A single ITR consists of palindromic arms (A-A’, B-B’, and C-C’) that... Deng, J., Hebben, M., & Liao, J. (2024). A Comprehensive Study of the Effects by Sequence Truncation ...

...integrated into the genome of the target cell or transiently expressed. It’s important to note that for all...vector can express your construct in target cells transiently or constitutively depending on the virus used...

...described in this publication We also wrote an extensive review on the exciting new field we call “precision...approach: Phenotypic Characterization of a Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L...

...And there are the usual transfection drawbacks: transient expression and needing to work with a transfectable...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

...monoclonal Cas9 background because you’ll have more consistent levels of Cas9 expression between experiments...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs ...

... and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from ...level, the potential for methylation should be considered when digesting DNA. Why? Well, even though Dam...

... in the human genome. The pair has focused considerable attention on transcription factors, starting... Myers: Only a small percentage of the proteins inside a cell have a commercial antibody against them....

...increase activity and decrease the gRNA size by intensively remodelling the gRNA scaffold through five rounds...tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable of correcting...

...even come [to grad school]!” He asked if she had considered other careers, which she hadn’t.  He then said.... “I was lucky to have been able to make that transition really smoothly,” says Susan. After being in ...

...your network. If someone has connected with me, I consider them fair game for connecting to others.  Pro ...including and especially the hiring manager. This is considered intrusive and it’s still connecting with a stranger...

...Streak on them an E. coli  strain known to be sensitive to that antibiotic, and if the strain is growing...positive clone after these steps, you might want to reconsider your approach, and try a different cloning strategy...

...phosphorylated MEK1/2 levels, suggesting PP1/PP2A is responsible for this MEK1/2 negative feedback. 3. Studying...Silvia Carrasco, and Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in...

...be used to measure your fluorescent protein’s propensity to aggregate. The basics and pitfalls of these... the best way to test a fluorescent protein’s propensity to homodimerize. Several studies have used the...

... transport miRNA from drug-resistant cells to sensitive ones. Though this process is not fully understood...malignancy, concluding that these cells may be considered "natural tumor suppressors." A growing interest...

...toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during cell division....E. coli do not contain the F plasmid (and are considered F-); however, there are a few popular lab strains...

...naturally in camels, consist of a single heavy chain, and they are small and stable inside cells. Nanobodies...

...abundance before and after a treatment. The library consists of a pool of lentiviral vectors with roughly 73...populations, they found that similar barcodes consistently made up the majority of the resistant populations...

...institutions. The conversations often move quickly into sensitive topics and uncomfortable places, from sticky ethical...rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull...

... 1000000097) The Winslow lab barcoding library consists of 96 retroviral plasmids (based on the Murine...mammalian cells. The MuLE system kit from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral...

...because it is non-pathogenic, well-studied and inexpensive. In addition, a complete set of well-annotated...different due to a whole genome duplication and extensive rearrangements in the S. cerevisiae lineage (7...