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We narrowed to 730 results for: NSI

Showing: 461 - 480 of 730 results

  1. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...selected to participate on this panel, but my consistent writing for the Addgene blog (~1-2 blog posts...social media, but how you use social media to consistently engage with others about your topic(s) of interest...

  2. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ... and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from ...level, the potential for methylation should be considered when digesting DNA. Why? Well, even though Dam...

  4. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...phosphorylated MEK1/2 levels, suggesting PP1/PP2A is responsible for this MEK1/2 negative feedback. 3. Studying...Silvia Carrasco, and Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in...

  5. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...be used to measure your fluorescent protein’s propensity to aggregate. The basics and pitfalls of these... the best way to test a fluorescent protein’s propensity to homodimerize. Several studies have used the...

  7. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...your network. If someone has connected with me, I consider them fair game for connecting to others.  Pro ...including and especially the hiring manager. This is considered intrusive and it’s still connecting with a stranger...

  8. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...Streak on them an E. coli  strain known to be sensitive to that antibiotic, and if the strain is growing...positive clone after these steps, you might want to reconsider your approach, and try a different cloning strategy...

  9. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ... transport miRNA from drug-resistant cells to sensitive ones. Though this process is not fully understood...malignancy, concluding that these cells may be considered "natural tumor suppressors." A growing interest...

  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...increase activity and decrease the gRNA size by intensively remodelling the gRNA scaffold through five rounds...tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable of correcting...

  11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during cell division....E. coli do not contain the F plasmid (and are considered F-); however, there are a few popular lab strains...

  13. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...abundance before and after a treatment. The library consists of a pool of lentiviral vectors with roughly 73...populations, they found that similar barcodes consistently made up the majority of the resistant populations...

  14. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...institutions. The conversations often move quickly into sensitive topics and uncomfortable places, from sticky ethical...rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull...

  15. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... 1000000097) The Winslow lab barcoding library consists of 96 retroviral plasmids (based on the Murine...mammalian cells. The MuLE system kit from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral...

  17. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...and mice with Nanoblades CRISPR has also been extensively used to create transgenic mice by microinjecting...post Resources on Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources...

  18. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...Special breeding cases Whether your mouse colony consists of genetically engineered mice with a single mutation...strains that have been crossed, there are many considerations for effectively breeding your mice. Because...

  19. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...scientists to confidently choose reliable and consistent antibodies for specific markers. The Screening...production methods    NeuroMab and others have an extensive library of monoclonal antibodies; Addgene collaborates...
Showing: 461 - 480 of 730 results