We narrowed to 798 results for: REP

...through the lens of Addgene’s blog and plasmid repository. Our blog is just over a year old, and it has...Doench (Guest Blogger) Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making...

...resources: Preserving scientific assets: We hold a repository of valuable research materials that might otherwise...offer high-quality DNA sequencing, viral vector preparations, and antibody production that labs may find ...

... SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or...control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor...

...wide variety of science careers that Addgenies represent. They kicked off their day with breakfast and ...see a real working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite...

... veritable virus factory.  Their findings were reported in the Journal of Virology.  The EBOV glycoprotein...offers sequence data on five strains of Ebola representing the latest outbreak and earlier ones, along ...

... works and, equally importantly, what doesn’t. Reports receive a DOI for citation, crediting researchers...sharing, information sharing, and research reproducibility while expanding our suite of materials and ...

...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...

...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ...

...and also to encourage women, students, and underrepresented minorities to study STEM subjects. Here, Sadangi...sci-art that would ultimately give a visual representation of science to our audience. How can researchers...

...We began distributing ready-to-use virus preps through our viral service in late 2016 and requests are...pAAV-hSyn-DIO-hM4D(Gi)-mCherry was the top requested AAV prep for the 2nd year running, and you can learn more...

...relationships because networking has gotten a bad reputation (as in…”I just hate networking”). Scientists ...interview you will be asking the questions, so be prepared. There are many good lists of questions on the...

...preference to journal access granted via a public repository or authors’ own web sites. 9)      The year 2013...plasmids more accessible and easier to share. As we prepare to ring in the New Year, let's make a toast to ...

...culture alone and relies on its algae host for reproduction. Snail parasite Leucochloridium is a parasitic...Richard C., and Matthew R. Gilligan. "Tongue replacement in a marine fish (Lutjanus guttatus) by a parasitic...

...the process by which nearly all domains of life repair genomic damage, specifically double strand breaks...site (TEV or PreScission), epitope tag (12xHis, 2xStrep, 3xFlag, Protein A, or V5), and selection marker...

...strategizing, designing, developing, testing - and repeat - we’re proud and excited to finally share with...unique: community, sharing, and fun. The Addgene repository is built on the foundation of a community that...

... Already we’ve seen articles about COVID-19 on preprint servers and in journals, and researchers have ...new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not only supports...

...most well-known being transcriptional activation/repression. dCas9 has also previously been adapted to fluorescently...distance! To the authors’ knowledge, this work represents the first mapping of intrachromosomal loci, a...

...for PIs to include in their annual NIH progress reports information on the Individual Development Plans...comments were about the astounding lack of reproducibility being recognized in published work even in ...

...have deposited their plasmids into the Addgene repository. More than 4,500 plasmids have already been deposited...aware of what Addgene can do for them and how the repository can help them share their materials. The new ...

...the process, that you are not losing library representation.” It’s also important that you infect enough...selection. Shalem and Sanjana say they kept a high representation of library within cells and aimed for 300 cells...