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We narrowed to 777 results for: REP

Showing: 541 - 560 of 777 results

  1. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...the process, that you are not losing library representation.” It’s also important that you infect enough...selection. Shalem and Sanjana say they kept a high representation of library within cells and aimed for 300 cells...

  2. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...scientific research are misidentified! Rigor and reproducibility of scientific data depends on validated reagents.... For instance, recently Cellosaurus received a report that HT-55, which was described as a colon cancer...

  3. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ... evolution, Tn7-like transposons captured and repurposed nuclease-deficient CRISPR-Cas systems to help...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....

  4. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...capstone project report and presentation, during the final week at Addgene. This report shared my work ...

  6. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...world choose to share their plasmids through our repository since we know biological resource centers, including... lack the resources or personnel to constantly prepare them for distribution. One of the major advantages...

  8. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...with implanted electrodes. The Archon1 voltage reporter allows a way to monitor neuronal electrical activity...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  9. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...) using in utero electroporation or with AAVs (prepared with Addgene #104110 - #104112) (Figure 2). We...with an index-optimized clearing agent." Cell reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke...

  10. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...systems during experiments – neither ideal for the intrepid researcher. There existed, therefore, a need for...disturbed, the brain fibers could not be directly repurposed for gut implantation. The fibers they ended up...

  11. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...taking beautiful photographs of their studies, reporting on amazing women in science, or asking someone...yourself open to conversation — put your work on preprint servers, share your email address far and wide...

  12. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...swapped for alternative genetic cargo, they can’t reproduce more functional viruses later on. These built-... is! Addgene has a number of ready-to-use viral preps available for a variety of broadly applicable and...

  13. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been in use since...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...

  15. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...ahead of time so that lab members have time to prepare their thoughts. Then at the lab meeting, the team...everyone stands up, disappears from the video, and prepares a coffee, or simply moves away from the desk to...

  16. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ... we found in our map have an impact on virus replication. While we and our collaborators are starting ...straight-forward. All constructs are tagged with a 2xStrep-tag for easy detection and affinity purification...

  17. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    .... Both tools were targeted to the FBN3 intronic repeat which contains 22 copies of the gRNA target site...this system out on chromosome 19 by targeting 46 repetitive loci that contained  ≥20 copies of the gRNA target...

  18. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...Make sure your cells are as healthy as can be to prepare them for the arduous process of generating viral...Resources on Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our...

  19. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...those of the guest blogger and do not necessarily represent the views of Addgene. I think it’s safe to assume...smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five...

  20. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...of simple biological machines R bodies can repeatedly extend and contract in response to changing pH... Biology Posts  Suggest Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids...
Showing: 541 - 560 of 777 results