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Addgene
Showing: 541 - 560 of 567 results

  4. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...our pipeline on a local computer or in a cloud environment. Given the throughput necessary to keep up with...

  5. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...most prominent idea, and instead building an environment where everyone has their ideas heard.  So how...

  6. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...References and resources References:  Björck L, Kronvall G (1984) Purification and some properties of streptococcal...

  7. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

  8. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...Repetitive sequences Restriction sites Sequences prone to create secondary structures in RNA transcripts...

  12. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...viable? Or cytotoxic? How and where did things go wrong? The use of proper control plasmids would address...

  13. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...in many FRET experiments. In addition, other environmental factors, such as pH or autofluorescence background...

  14. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...method is single strand annealing (SSA), an error prone mechanism that notably does not require a donor ...

  16. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...referred to as sequence DNA as it synthesized by electronically recording fluorescence activity as fluorescently...

  17. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...Off-target effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389...

  19. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... are also considered to be less stable and more prone to being lost in bacterial cells compared to monomers...
Showing: 541 - 560 of 567 results