We narrowed to 582 results for: RON

...Agrobacterium vectors for plant synthetic biology’, Frontiers in Plant Science, 4. doi: 10.3389/fpls.2013.00339...

...CRISPRko libraries are highly effective at identifying strong hits in genetic screens, they do have several limitations...

...  Ashley Waldron...

...product, then you know there could be something wrong with the PCR setup and/or the primer design. ...

... proteins at once.  However, as Y2H screens are prone to both false positive and false negatives, these...

...collections. Many companies, such as Thermo Fisher and Micronic, provide 2D barcoded tubes. 1D barcodes are better...

...Japanese science. I really miss the intellectual environment in the US in this regard. In addition, not many...

...what you don’t know – i.e., if they use a term or acronym you don’t know, or if they assume you have done...

...protocols therefore differ from library to library. We strongly recommend following the protocols provided by ...

...most prominent idea, and instead building an environment where everyone has their ideas heard.  So how...

...References and resources References:  Björck L, Kronvall G (1984) Purification and some properties of streptococcal...

...Escherichia coli Genome via the CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128...

...we launched the Addgene YouTube channel to add a strong visual component to our educational resources. ...

... mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine et al developed...

...PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (...

... people and make you work in a collaborative environment. Think of student groups, PostDoc associations...

...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

...Repetitive sequences Restriction sites Sequences prone to create secondary structures in RNA transcripts...

...et al. show that genome-wide CRISPR screens are prone to some of the same flaws as RNAi screens, such ...

...., Milanova, D., Kerby, M. B., Snyder, M. P., & Barron, A. E. (2011). Landscape of Next-Generation Sequencing...