We narrowed to 580 results for: RON
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TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....CRISPRko libraries are highly effective at identifying strong hits in genetic screens, they do have several limitations...
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Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....product, then you know there could be something wrong with the PCR setup and/or the primer design. ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m.... proteins at once. However, as Y2H screens are prone to both false positive and false negatives, these... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....collections. Many companies, such as Thermo Fisher and Micronic, provide 2D barcoded tubes. 1D barcodes are better... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....Japanese science. I really miss the intellectual environment in the US in this regard. In addition, not many... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m.... mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine et al developed... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....., Milanova, D., Kerby, M. B., Snyder, M. P., & Barron, A. E. (2011). Landscape of Next-Generation Sequencing... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....slow-growing cells, particularly mammalian cells like neurons, may not replicate during the course of your experiment... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....we launched the Addgene YouTube channel to add a strong visual component to our educational resources. ... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....Synergistic Activation Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....our pipeline on a local computer or in a cloud environment. Given the throughput necessary to keep up with... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....most prominent idea, and instead building an environment where everyone has their ideas heard. So how... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....References and resources References: Björck L, Kronvall G (1984) Purification and some properties of streptococcal... -
How to Be an Excellent Trainee
TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m....what you don’t know – i.e., if they use a term or acronym you don’t know, or if they assume you have done... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....protocols therefore differ from library to library. We strongly recommend following the protocols provided by ... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....think this is represented by factorials. In environmental science and ecology, this can be represented... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m....be exposed to asbestos. However, as the U.S. Environmental Protection Agency has noted, there is no safe... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....Escherichia coli Genome via the CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...