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We narrowed to 749 results for: SOM

Showing: 541 - 560 of 749 results

  1. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your screen...

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...Creative titles and controversial topics can garner some attention, though in reality much of our research...about your research is not hard either. Here are some resources: ImpactStory, a website that helps researchers...

  3. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...the independent variable you are testing and not some other unintended factor. Control plasmids are used...different days, etc, but outside constraints may impose some limitations and you should be cognizant of these...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...configurations can generate functional scFvs; however, some individual scFvs performing better in one configuration... of screening. Created with Biorender.com.   Some phage display libraries screen antibody genes from...

  6. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...them back to wild type), differentiate them into somatic cells affected by the disease and study what’s ...guide RNA binding site works well. However, since some guide RNAs may tolerate a mismatch in the binding...

  7. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...nucleus, or visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely...depending on the location of the targeted markers. Some commercially available fix/perm kits allow for labeling...

  8. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...bind discrete subunits or particular subunit pairs. Some of the antibodies in the collection are able to ...hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) chimeric antibodies...

  9. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...plant breeding approaches, these techniques are cumbersome, often requiring several rounds of selection ...functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components are ...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... was really due to the intended edit rather than some passenger variant that came along with the single...likely to be active, not only for SpCas9 but also for some other Cas enzymes. It seems to be the case that ...

  11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...of the vector. In order to reduce the chances of some clones being outcompeted by others in liquid culture...extraction reagent (BPER from Thermo works well) with some added lysozyme, DNAse I, and protease inhibitor ...

  13. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...work than using established methods and minimizes some of the technical issues associated with high levels...Classic genetic engineering methods enabling chromosomal integration of sequences in bacteria are time-consuming...

  14. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...'s out there, Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses...

  18. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...Twitter, so keep an eye out there for a chance to win some cool Addgene swag.  And, of course, to all our depositors...

  19. The Twelve Days of Antibodies

    Type
    Blog Post
    Published
    Jan. 6, 2025, 5:17 p.m.
    ...Every year, we like to add in some holiday cheer to our blog and social media. This year, we're doing...

  20. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...we have added new sections to share and describe some incredible new CRISPR technologies developed and...
Showing: 541 - 560 of 749 results