... ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. e98635. PubMed PMID: 24901218. PubMed Central PMCID...2015): 2321-2332. 3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second...

...Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides and an NgAgo expression plasmid to edit...endonuclease) protein that can form a complex with a 5’-P-ssDNA guide at room temperature. Until that time, ...

...research   Optimized prime editing in dicots By Emily P. Bentley Prime editing (PE), initially developed in... H., Natale, S., Gomez, L., Taxidis, J., O'Neill, P. S., Villette, V., Bradley, J., Wang, Z., Jiang, D...

..., Public Library of Science (PLoS), 18 Feb. 2011, p. e16765. Crossref, https://doi.org/10.1371/journal.pone...5, Public Library of Science (PLoS), 30 May 2018, p. e0197185. Crossref, https://doi.org/10.1371/journal.pone...Communications, vol. 2, no. 2, Elsevier BV, Mar. 2021, p. 100135. Crossref, https://doi.org/10.1016/j.xplc.2020.100135...

...membrane stacked behind the p membrane. If your protein runs through the p membrane into the psi membrane...membrane for small proteins (< 15 kDa) and a 0.45 µm (p) membrane for larger ones. Changing your membrane ...membrane, that’s a good indication that the p membrane pores are too large.  Transfer method   Wet transfers...

...Hickok, G. H., Lemmen, A. M., Nguyen, T. H., Prathima, P., Fithian, W., Schwartz, M. A., Hacohen, N., Doench...

...Niehaus in December 2015 and was updated by Emily P. Bentley in June 2024.  References and resources ...J. E., Rozgaja, T. A., Gupta, R., Dyson, K., Rao, P., & Leonard, A. C. (2018). Origin recognition is the...

...potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways...Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014). PubMed. The...

.... https://doi.org/10.1038/nature02426 Iannaccone, P. M., & Jacob, H. J. (2009). Rats! Disease Models &...Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian model organisms...

...Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin...

...were run on a western against a protein standard (P) to check for tau molecular weight shifts. A ...PubMed Central PMCID: PMC4212362. Alexander, Steve P. H., et al. “Goals and Practicalities of Immunoblotting...

..., Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa, A. A., Qu, C., Zhu, Y., Guan, Y., Wang... Emily P. Bentley...

...incorporated into the full-length protein. Up to 80% p-Ser incorporation into E17TAG GFP has been achieved...incorporation due to differences in stability of the p-Ser incorporated protein as well as context-specific...

...subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature...with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet ...

...Use active listening skills such as the PAC Method. P = demonstrate patience, pause; A = ask at least one...

..., De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios X, Huang P-Y, Daley G, Church G (2013...

... References and Resources References Andrews, N. P., Boeckman, J. X., Manning, C. F., Nguyen, J. T., ...protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An Introduction to Performing...

...doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted delivery of nanomaterials...doi.org/10.1038/s41467-020-15731-w Wang F, Wang C, Liu P, Lei C, Hao W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced...

...Staber, S. J. Wheelan, J. K. Joung, P. B. McCray, F. D. Bushman, P. L. Sinn, and N. L. Craig, “piggyBac...

...producing difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins...

...Bandrowski, A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F., Bradbury, A. R. M., Cable, H., & Longworth...

...loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci...

...CRISPR-Guide RNA Library. Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology...

...Hurtrel, B., Heard, J.-M., Rivière, Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent... Hauswirth, W. W., Boye, S. E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve...

...797459. Berg CM, Liu L, Coon M, Strausbaugh LD, Gray P, Vartak NB, Brown M, Talbot D, Berg DE. pBR322-derived...Crozat E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution of Multimeric Forms of Circular Plasmids...

... Bohn, P., Joines, K.M., Peng, H., Hawrylycz, M.J., Phillips, J.W., Hohmann, J.G., Wohnoutka, P., Gerfen....L., Kieran, D., Redmond, D.E.Jr., and Aebischer, P. (2010). Efficient transduction of non-human primate...

... https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’...

... https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020. ...

...Zhang, Y., Dong, A., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin,...

...4594039. PubMed Central PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids...

...vectors The vast majority of fly vectors are based on p-element, random or targeted, integration into the ...

...Central PMCID: PMC1197021. Zhao, N., Kamijo, K., Fox, P. D., Oda, H., Morisaki, T., Sato, Y., … Stasevich,...

..., S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher, ...

...without those critical three letters after your name (P-H-D).  Finally, having spent your entire career in...

...subtelomeric region of the pericentromeric region (PR1), the p-arm (T1), and the q-arm (T2) of chromosome 19. Image...

...J., Reynolds, H., Bryson, C., Fogg, M., Baker, M. P., Herskovitz, J., Goletz, T. J., Zhou, L., Moxness...

....1704882114 Kahan, D., Peters, E., Wittlin, M., Slovic, P., Ouellette, L. L., Braman, D., & Mandel, G. (2012...

....3389/fmed.2023.1106085. PMID: 36817775. Wilmott, P., Lisowski, L., Alexander, I. E., & Logan, G. J. (...

...Broad was building that had Platforms with a capital ‘P’ focused on technology ... has been critical to the...

...counting plasmids here References Leonard, Sean P., et al. "Genetic engineering of bee gut microbiome...