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We narrowed to 507 results for: CIT

Showing: 41 - 60 of 507 results

  1. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ... to no toxicity in the knockout line, it is likely a specific drug with low off-target toxicity. This ...candidates are isolated, they can be validated for specificity to their target, or their direct target can be...developed, it’s good practice to measure the specificity of that target and validate the hit compound....toxic to both lines, it probably has either high toxicity or high off target effects, both of which make...keeps advancing, so will drug discovery – it’s an exciting time to be in the biomedical research field! ...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...interest. Although they have a small packaging capacity, AAV vectors were suitable for our needs for the...vivo animal model should consider the possible elicited immune response from viral infection. Animal safety... also have similar transport characteristics (velocities of movement, distance traveled, etc.) (4). This...identified with AAV2 (5), AAV6 (6) and AAV5 at excitatory connections (3). These facts beg the question...potential of AAVs is their ability to be used with new exciting technologies (such as CRISPR/Cas9) as they allow...Joshua C., and Richard J. Samulski. "Packaging capacity of adeno-associated virus serotypes: impact of...recombinant adeno-associated viral vectors: packaging capacity and the role of rep proteins in vector purity....

  3. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...Editing Specificity” by Tycko et al. 2016 and “Defining and improving the genome-wide specificities of CRISPR–Cas9...have been widely adopted due to their relative simplicity and low cost. The problem with these assays is...cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature biotechnology 31.9 (2013): 839-843. ...genome-wide analysis of zinc-finger nuclease specificity." Nature biotechnology 29.9 (2011): 816-823. ...Joung. "Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases." Nature Reviews Genetics17.5...Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity." Molecular Cell 63.3 (2016): 355-370. PubMed...

  4. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ....   Excitation with NIR light also produces less non-specific ROS because NIR light doesn’t excite natural...2I-dL5**).   A FAP-TAP complex will light up if excited with light of specific wavelength and also produce...  The iodine-modifications also shift MG-2I’s excitation wavelength to 666 nm, which is within the near-infrared... non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could be ...increase cell ablation efficiency, but dL5** phototoxocity is induced when expressed on the cell surface...

  5. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...contrast, fluorescence occurs when a molecule is excited by a high-energy photon and re-emits a lower-energy...proteins. On the other hand, they don’t require an excitation light source (usually a laser in fluorescence... Background Lower Higher Photobleaching/phototoxicity Not susceptible Susceptible Subcellular imaging...Transfer (FRET) is the transfer of energy from an excited donor fluorophore to an acceptor fluorophore, which...to a nearby fluorophore, which emits light. No excitation illumination needed — just the luminescent substrate...live-cell imaging by avoiding autofluorescence and phototoxicity and even allowing multiple proteins to be visualized...

  6. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...photobleaching and phototoxicity that standard FPs might induce though their continuous excitation. By generating...chromophore which contribute to the 2 peaks of wtGFP’s excitation spectrum- a major 397 nm peak and minor 475 nm...anionic form, causing increased fluorescence when excited with 488nm blue light. The T203H mutation in PA-GFP...Contrast (Fold Change in Fluorescence) Brightness Excitation/ Emission (nm) PA-GFP Monomer UV-Violet Dark...Contrast (Fold Change in Fluorescence) Brightness Excitation/ Emission (nm) Dronpa Monomer UV-Violet Dark...own quirks (level of zoom, intensity, possible excitation lasers) it is also important to optimize your...

  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...AAV components      Cargo capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your...or not infect) different cell types with some specificity. There are a lot of AAV serotypes, many of which...or 2A to co-express them from one promoter. Specificity The ability of AAV to drive gene expression in...or tissues is one of its biggest advantages. Specificity can be achieved using specialized promoters, ..., and/or serotype can also be used to ensure specificity; this is particularly helpful when wanting to...

  8. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...the host genome, removing the risk of oncogenicity or genotoxicity seen with other vectors (Lukashev et...large transgene packaging capacity. For instance, with a packaging capacity of about 6 kb, the 1st generation...AdVs have already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the...

  9. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ... permanent residence case with United States Citizenship and Immigration Services (USCIS). If it were ...researchers who require visas, permanent residence or citizenship to continue their work in the US have the added...objective criteria including awards, media attention, citations to your work, downloads, commercialization of ...to join (selected due to expertise in field). Publicity in major media and/or trade journals: You the ...platinum standard for an expert is someone who has cited your work. Immediate steps that you can take to ...working visas, permanent resident green cards and citizenship.   Lauren Celano, CEO Propel Careers. Lauren...about working with scientific individuals to find exciting growth opportunities in life sciences       Propel...

  10. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...regeneration into one of my own courses, I was excited to hear about how he developed his program. Initially... students work on malate dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school... to identify regions of MDH that interact with citrate synthase and presented their findings at the American...the research process – so if this sounds like an exciting opportunity for your classroom, I highly recommend... Wallert, M., Provost, J. 2010a. Bringing the excitement and motivation of research to students; Using... Wallert, M., Provost, J. 2010b. Bringing the excitement and motivation of research to students; Using...

  11. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...induce cell death. CRISPR also enables increased specificity - rather than targeting a process essential to...like for organisms like C. difficile. In 2014, Citorik et al. and Bikard et al. developed sequence-specific...antimicrobial, plasmid-based, CRISPR systems (Find the Citorik Plasmids here). In both cases, they used 1-2 CRISPR...sequences found only in certain bacteria (Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance...beta-lactam resistance genes, again with very high specificity. This double phage system both favors antibiotic...fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy K. Lu. “Sequence-specific...

  12. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...ssAAV). To maintain the packaging capacity and improve the specificity of ssAAV, a targeting sequence of...As research has just begun with PE7, it will be exciting to see future studies enabled by this tool as ... in an AAV vector (Figure 5). Efficiency and specificity of these AAV vectors for microglia was further...mice. B) Quantifications showing high microglia specificity of GFP expression for the indicated ssAAV5s. ...reconstitute the gene of interest, reducing the immunogenicity typically associated with viral vector-based...

  13. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ... humanities, and 32% in the social sciences go uncited. Creative titles and controversial topics can garner...since the 1980s researchers have been increasingly citing research from outside of their disciplines. Included...at the same time increasing interdisciplinary citations in their own work. Of course not all research ...ImpactStory, a website that helps researchers go beyond citation rates to measure the impact of their contribution... the WeShareScience project is to find new and exciting ways to share ideas derived from research. The...Archambault. "The decline in the concentration of citations, 1900–2007." Journal of the American Society for...

  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ..., you can easily directly excite the acceptor with the laser used to excite the donor. In that case the... energy is transferred non-radiatively from an excited molecular fluorophore (the donor) to another fluorophore...between the two fluorophores is linked to their excitation spectrum overlap. If you choose a pair too close...sensitised emission method, where the donor is excited by a specific wavelength of light and the signal...method is also less sensitive to direct acceptor excitation artifacts and it is possible to use a non-fluorescent...

  15. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...promoters with no tissue specificity, so in some cases they can lead to toxicity and off target effects.... flexibility in expression levels and tissue specificity. Using your shRNA plasmids One of the most common...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...

  16. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...Bicistronic vectors combine excitation and inhibition by using both excitatory and inhibitory channels activated...Light activated ion channels (rhodopsins) either excite or inhibit neuronal activity upon exposure to light...light within a specific range of wavelengths. Excitatory channels allow positively charged ions such as...wavelength, turns it off, allowing the cell to be excited over a sustained period of time (Gong et al., 2020... the opsin.      Figure 2: Diagram of excitatory (ChR2) activation by light showing ions flowing...

  17. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...proteins and this interaction determines the specificity and efficiency of the AAV serotype used for a...Combining different approaches can increase the specificity of cell targeting even further. For example, ...AAV at the injection site results in increased specificity of the targeted cell population.  Combinatorial...improve delivery by increasing transgene cargo capacity, enhancing anterograde transport efficiency, and...testament to openness and sharing in science.  We’re excited to support the neuroscience community by sharing...

  18. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...arrived, hooray! Before you jump straight into the exciting new experiments that you’ve been planning, it ... to molecular targets with high affinity and specificity. However, there are countless variables that ...that impact an antibody’s affinity and specificity for a given target and it is all too common for antibodies...experiments for every antibody that you receive; citing existing validation data can be acceptable. However...binds to what you expect it to bind to. Show specificity for the target antigen - Just because an antibody...something unexpected. This approach has high specificity, but you need to be aware of any possible alternative...

  19. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...Being excited about the science allows you to ask more interesting questions and your excitement will ...us everything there is to know is a strange and exciting revelation. Science in any field can generally...vague sense of what kind of research you would be excited about. I know quite a few friends who opted to ...people actively seek out science that genuinely excites them, and reach out to the PI to get involved. ...their research, and learn about what makes them excited to do what they do. I would also highly recommend...

  20. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...Antibody dependent cellular cytotoxicity (ADCC) Antibody dependent cellular cytotoxicity (ADCC) is a slightly...deposition (ADCD) b) antibody-dependent cellular cytotoxicity (ADCC) c) antibody-dependent cellular phagocytosis...JV. Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor targets. Nat Med. 2000 Apr;6(4... constant region contributes to affinity and specificity. Trends in Immunology, 29(2), 91–97. https://...
Showing: 41 - 60 of 507 results