Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 402 results for: LARGE

Showing: 41 - 60 of 402 results

  1. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...United States, I look forward to gathering around a large table for a meal with my family. I am thankful for...things, and one is that I get to gather around a large table to eat with my Addgene family every day. Do...

  2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...removing the full ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for ... same time (Liu et al. 2018). However, removing large lncRNA fragments with multiple gRNAs poses the risk...Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in: insertion of a synthetic...

  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ... SPH mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators...activators, which largely precludes delivery by adeno-associated vectors (AAV). This issue was recently circumvented...delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was...

  7. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...tools for efficient and programmable insertions of large DNA cargos into bacterial genomes. This new capability...CRISPR-transposons excel at programmable insertions of even very large genetic cargo, at one or multiple desired genomic...systems have already been demonstrated, ranging from large genomic insertions and/or deletions, to multiplexed...

  8. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...III-E, and then used this information to identify a large variety of related bacteria with similar Cas systems...fused integrase would then integrate into with a large DNA insert – or Programmable Addition via Site-specific...programmed for new genes. In comparison with other large insert techniques like HITI or HDR, which require... and adenovirus templates. The system, which is larger than Cas9, can also be used with mRNA and a synthetic...

  9. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together, these two requirements...interactions identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with...chromatin interaction networks since they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing...

  10. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...defects in the gene MYO7a, at ~7 kb this gene is too large to deliver via AAV.  Dual vectors strategies are... to regulate and optimize transgene levels, and large scale manufacturing of viral vectors to support ...cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot be delivered using this vector...

  11. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ... in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce both...the clinic Since lentiviral vectors can deliver a large amount of DNA (~8 kb) with a relatively low immune...lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector but for ...Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien...

  12. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...detection; however, those are just one (admittedly large) class of common fusion protein tags. Biochemists...; has inherent enterokinase cleavage site GST Large Protein 26 Purification and Stability Good for purification..., flanked by hexahistidine motifs (6xHis) MBP Large Protein  40 Solubility and Purification Can improve...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...observed to occur more frequently in plasmids with large inserts (>7 kb)(Berg et al., 1989), direct repeats...reads (<300 bp) which cannot distinguish between large repeat sequences within a plasmid, it cannot be ...sequencing. These longer reads are able to detect large repeat sequences within a plasmid and, therefore...DE. pBR322-derived multicopy plasmids harboring large inserts are often dimers in Escherichia coli K-12...just as well as monomers. However, due to their larger size, multimers may exhibit reduced transformation... & Sherratt, 1984; Summers et al., 1993). These larger plasmids are also maintained at lower copy numbers...monomeric version. However, undigested multimers are larger than monomers and will therefore run higher on ...

  14. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ... that cellular mismatch repair contributed to a large number of unintended prime editing outcomes, including...combine prime editing with a recombinase to install large sequences (up to 10 kb) into the genome (Pandey ...deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nature Biotechnology... (2024). Efficient site-specific integration of large genes in mammalian cells via continuously evolved...J. S. (2023). Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using...

  15. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...overheating remains an issue, which can happen with large proteins, try lowering the voltage and using a longer...you know you loaded a good amount of sample of a large protein, you may be under-transferring.  Membrane...good indication that the p membrane pores are too large.  Transfer method   Wet transfers are more efficient...efficient, so if you’re struggling to transfer a large protein, consider using a wet transfer with a long... lot of antibodies. If you’re planning to use a large amount of a specific antibody, plan ahead and request...harsher stripping buffer Too little signal Large proteins: Incomplete transfer Check transfer with...proteins going completely through the membrane or larger proteins not transferring completely onto the membrane...

  16. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...plasmids as they grow, scientists can easily make large amounts of plasmid to manipulate and use in later...that plasmids can be used to do include: Produce large amounts of a protein so that scientists can purify...

  17. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ... small deletions, while H840A is biased towards large insertions. These profiles are likely due to the... Oligonucleotides) Use 100 nt homology arms for large insertions (long ssDNA; IDT Megamer® ssDNA Fragments...

  18. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...fluorescence. It is useful for activity imaging of large populations of densely labeled neurons because background...fluorescence.  It is useful for activity imaging of large populations of densely-labeled neurons because background...accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR...

  20. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...to efficiently deliver large repair templates for applications that require large gene fusions. The current...Matthew H. Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports...
Showing: 41 - 60 of 402 results