We narrowed to 771 results for: SOM

...style match for your needs can take some research.  It helps to spend some time thinking about what your goals...coaching plans that are financially accessible. Sometimes only a few hours of coaching time can make an ...know who have had a successful coaching experience? Some university career offices, postdoc offices or professional...vouch for them. Trust your gut – only work with someone you connect with. Talk to the coach about their...for clear information on costs. Can you suggest some coaches? Yes I can. Please note that Addgene does...LinkedIn and Facebook to see their online personas. Some have online coaching tools that can be very effective...Maybe you just need help getting through your PhD? Some coaches focus on graduate students. For example ...

...by offering advice on areas where you might need some help. Today in our transferable skills guide: Negotiation...grad student, I thought that “negotiation” was something that I would mainly do over the price of a used...unprepared. Mainly, I thought of negotiation as something done not by people wearing a lab coat, but instead...transferable skill? Negotiation as a zero sum game Some people think about negotiation as a zero sum game... argument but felt bad afterwards). Or worse, sometimes there’s no agreement at all and everyone leaves...for this person’s request? Your colleague is if someone can feed their cells this weekend - why? Do they...plans? What is most important to each person? Someone has to be the first author but another author can...

...a vector backbone containing a topoisomerase I cut site, toposisomerase I, and a taq polymerase-generated...There are some cloning methods that make working with multiple inserts a breeze. Among them are some of the...consideration is: The number of inserts ...as some methods are more amenable to combining multiple ...polymerase-generated insert. When mixed, topoisomerase I provides both the endonuclease and ligase function while 5’ ...overhangs left by Taq perfectly anneal with the topoisomerase I generated overhangs in the backbone. Once ...Origene, and NEB® are all good places to look for some helpful kits! Efficiency: Wouldn’t it be nice if...successful result? While there are never any guarantees, some cloning techniques do tip the scales in your favor...

...how E. coli normally replicate their chromosomal DNA. Chromosomal replication in bacteria is carefully...opposite end of the chromosome, ending replication.   Figure 2: Prokaryotic chromosomal replication. ...synchronized with the replication of the host cell's chromosomal DNA.  Plasmids are said to be under stringent...the first step of replication for the bacterial chromosome and for stringently controlled plasmids. Binding...replication fork. Termination sequences present in the chromosomal DNA ensure that the helicases are released from...regulatory mechanisms allow E. coli to control chromosomal replication and balance population growth against...controlled and has a low copy number. Like the E. coli chromosome, it has a single ori and requires the host proteins...

...vector remains episomal, meaning it stays separate from the genome. One option for episomal vectors is to...prokaryotic ribosome, while G418 (geneticin) is effective at inhibiting the eukaryotic ribosome. One resistance...will be lost from the population. A bacterial chromosome has a single origin of replication (ori), where...lines that express viral machinery. Alternatively, some slow-growing cells, particularly mammalian cells...but it won’t allow you to select for your vector. Some antibiotics block important cellular pathways shared...across kingdoms by targeting a shared aspect of ribosomal function. A puromycin resistance gene on your ...vector to survive while the auxotrophic cells die. Some auxotrophic selection markers can be used across...

...trainee possible? It’s not a mystery – there are some practices that can really help! Following best trainee...Typically, you will identify a ‘scientific mentor’: someone who provides career advice and offers direction...receive technical mentorship from a ‘bench mentor’. In some instances, the bench mentor is also the scientific...papers or protocols yet, don’t be scared to ask for some! You can email or ask in-person “Are there any papers...understand the purpose of, or a different way of doing something then you’ve done previously) you can bring those...especially when you are in a new place, learning something new. So how can you remember everything? Write...you have questions or need a little assistance at some point during the procedure. Be an active partner...

...by offering advice on areas where you might need some help. Today: Career Planning Resources. The key ... do now is diversify. Read on for an overview of some of the resources you can use to better prepare for...beneficial during my own graduate career – and in fact, some of the very things that were so critical to me landing... your CV or business card. Be warned, though – someone accepting your CV doesn’t necessarily mean that...elaborated on later. As for what job fairs to consider, some of the best options are the Naturejobs Career Expo...Biospace holds career events all over the country; sometimes several per month, giving you a plethora of opportunities... Post your CV and make it visible, which will sometimes result in a recruiter giving you a call. Many ...

... are aware of a problem or a need for something, usually someone else has realized this too. Begin with...by offering advice on areas where you might need some help. Today in our transferable skills guide: Collaborating... To get this and other projects like it going, someone (likely several people at Addgene) had to take ...accomplish a variety of goals. For example, I worked with some microscopy enthusiasts to start a microscopy seminar... about your ideas and don’t be afraid to make something concrete happen. You may have more willing collaborators...department, I personally felt that I had to overcome some hesitation to ‘approach strangers’ and learn how...companies and then convince the contacts to give me some time to talk or to at least read my emails. Then...

...best for you. Some of these important questions will be easy to ask the lab head, and some are better asked... the right lab such a big deal? It’s actually something you CAN choose and it will make a huge difference... level of attention and interaction (and I did). Some of the information you will need is about you. It...before making this important decision. I’ll highlight some of these questions in the sections below. Mentoring... help identify your priorities and needs, but sometimes it just takes introspective thought on what’s ...responsibility to train you seriously. Why work for someone who you don’t look up to and who doesn’t care about...performance and learning? Everyone’s different so give some thought to what atmosphere makes you successful....

...alleles. Some alleles, such as Cre, function when hemizygous, meaning that only one chromosome needs to...randomly into their genome, some transgenic strains are difficult to breed. Some problems with transgenic... if the mutations are on the same chromosome. Homologous chromosomes recombine during meiosis, creating...homologous chromosomes in the parental generation, they are likely to stay on separate chromosomes in future...distance between the two loci of interest on the chromosome, the less likely crossing over is to occur between... which would get both mutations onto the same chromosome. Until crossing over occurs between the two loci...

...integrity. (Not the case, as I’ve discovered.) As someone who has now seen both sides of the postdoc experience...decision a little easier if an industry postdoc is something that you’re considering. Like anything, there ...can’t afford to spend too much time working on something extremely risky or exploratory. Getting a job ...experience” that I’ve seen listed as a requirement on some industry job postings. Also, if you work in industry...their pipeline projects. In this scenario, finding someone with whom you can have an in-depth discussion about...Genentech, among others, and the list is growing. Some post positions as they come up, whereas others post...companies. Like most jobs, it does help if you know someone who’s already in industry to land you that position...

...’s no genomic integration Replicating EBNA1 episome Non-integrating Medium Medium Generates transgene-free...integration Inefficient Need to verify loss of episome Minicircle Non-integrating Medium Medium Generates...-deletable or Tet-inducible lentiviruses address some of these concerns, but overall integrating viral...for translational use. Viral Vectors Retroviral: Some of the first iPSCs were generated with Moloney murine...cut and paste” mechanism between a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb. This... Sendai virus transgene expression. Transient episomal delivery Non-integrating methods have a smaller...oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids carry the origin of replication...

... come across a couple other terms that can cause some confusion. Paraformaldehyde: Many IF protocols ...your sample, cross-linking can block the epitope of some targets, preventing your primary antibody from binding...antibody binding by tweaking those parameters, but in some cases your antibody may just not be suitable for...organic solvents may improve antibody binding for some targets, they can destroy it for others. That said...tough-to-permeabilize membranes and these detergents avoid some of the negative effects caused by organic solvents...BioRender.   How do I choose?! Ok, so now you know some of the possible types of fixatives or permeabilizers...but how do you decide which ones to use? Here are some tips for making those decisions: Always always ...

...discussed in Chapter 11)? Even if some of the problems are addressed in a somewhat cursory manner, their inclusion...Zimmer to introduce any one of these topics without some editorializing, and he wisely makes no such attempt... is, presumably, why they ended up in this book. Some of these issues are not particularly new. Chapter...science would be complete without the inclusion of some interesting case studies, and Zimmer provides several...sections on optogenetics and CRISPR of course address some of the ethical implications of using these technologies...changing nature. Full disclosure: Zimmer also spends some time on Addgene’s role in the rapid dissemination...suggestions are not so much inaccurate as already somewhat dated. Zimmer could not have predicted COVID-19...

... similar protocols, and look - they’re seeking someone who has, to a t, all the research skills under ... but also transferable skills, before offering someone their dream job; They want to know if the candidate...skills.” It is nice when other people point out how awesome we are, but there is also value in knowing and ...prepared for new and exciting opportunities. Here are some strategies for identifying your transferable skills... and using your charisma to give people hope for some shiny vision on the horizon. You’re about to keep...happens at your organization, when your boss needs someone to step up, when a new venture presents itself ...feedback, bask in the glow of a job well done, take some time to think, and write your thoughts down! What...

...reports where someone tries to “optimize” a particular standard lab protocol. For example, someone may attempt...often have to start pursuing other options. While some non-academic career options require additional study... answer loads of questions, and I also had to do some troubleshooting as advised by the technical support...required for a role in technical support? It takes some time (several years) to build up comprehensive lab...companies have dedicated technical support teams, sometimes comprised of several layers of support: A front-line...often lead to otherwise unobtainable solutions. To some extent there is the need (or opportunity) to get...listen to the many individual stories and get (sometimes refreshing) insights into how other scientists...

...s natural that some of us have more in-depth knowledge of one area, and when something urgent comes up... things in our code. In some parts of our code, we might be calling something a sample and in other places.... We support everyone that works with Addgene in some way. For users, we just released a major redesign...genders, educational, and professional backgrounds. Sometimes this can lead to wildly different ideas of how...during checkout. Demo details for this would be something along the lines of showing that the checkout takes... decide how to best approach it in our code.  Sometimes while thinking through these steps, we discover...fixing bugs: we take time during each sprint to do some maintenance work on our code. One way to look at...

...also require some additional optimization as each antibody performs differently and some antibodies impact...of interest within an intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based...are essential. In this article, we’ll go through some of the most important considerations to make when...see fluorescence indicates your target’s location. Some other terms you might see associated with IF are...visualize the antibodies using fluorescence (for some background on fluorescence, try the Introduction...straightforward than choosing a primary, so we’ll start there. Some of the most important considerations are to make...applications, including IF (or IHC or ICC), and will share some of their results and corresponding protocols. Other...

...resistance cassette. This leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding...There’s an astounding number of ways to create chromosomal mutations in bacteria, so many that it may be...quick and easy way to introduce a mutation in the chromosome is to disrupt expression of a gene with an antibiotic...ones that don’t leave any undesired scars in the chromosome. These methods roughly fall into two categories...introduced from a conjugative plasmid replaces the chromosomal copy. This is a versatile technique, and is less...large amount of user-to-user variability, and in some cases, counter-selection escape can occur: single...inserted a copy of rhaS with a strong synthetic ribosome binding site (using the online calculator from...

...First, you’ll need to find someone who is willing to host you! If there is someone you personally know or ...scientist? A visiting scientist, in academia, is someone who visits an institution to perform research there...is because the host lab or institute provides something that you can’t obtain at your current institute...research needs, that is probably the easiest path. Some institutes have structured visiting positions within... These structured types of placements often have some form of built-in professional development. These...be right or necessary for many scientists! Unless some of these logistics are covered by a structured visiting...notes, and ask questions if you don’t understand something. Offer to help out wherever you can and don’t ...