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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 293 results for: gne

Showing: 41 - 60 of 293 results

  1. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...biorender.com   Another method of harvesting uses magnetic beads. In this method, beads are incubated with...suspension. Antibodies bind ligands on the beads and a magnet is used to separate the beads from the media (Brechmann... Chotteau V. Antibody capture process based on magnetic beads from very high cell density suspension. ...

  2. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... Hub is here to help!  The Addgene Data Hub is designed to help scientists understand how our vectors ...images demonstrating vector performance) will be assigned citable DOIs through DataCite before being made...the Data Hub. These minimum data standards are designed specifically to allow scientists to evaluate how...

  3. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...Ludwig-Maximilians-University in Munich and Marc Tessier-Lavigne’s lab at the Rockefeller University in NYC. The ...project while he was a Postdoc in Marc Tessier-Lavigne’s lab at the Rockefeller University in NYC.   Dylan...Dylan Kwart is a graduate student Marc Tessier-Lavigne’s lab at the Rockefeller University. He currently...

  4. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...increase the frequency of edits, researchers have designed self-cleaving plasmids that liberate the targeting...information on HDR and CRISPR. Our CRISPR 101 series was designed to help explain the basic principles driving CRISPRs...et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

  5. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...comments section.  FX cloning kit This kit is designed to be used with Type IIS restriction enzymes and...et al. "A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo...(2015): 31-37. pCri system The Gomis-Ruth lab designed the pCri system to simplify the protein purification...

  6. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren Looger and Kaspar Podgorski...Aggarwal et al., 2025).In this latest work, the team designed and screened a large library of iGluSnFR3 mutants... HA-, and EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these...

  7. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two imaging probes. One consists of NLuc fused...Reporters. The LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection...acceptor, the more powerful the FRET signal. In well-designed FRET-based biosensors, the intensity of the FRET...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...domain (FokI). Zinc finger nucleases were first designed in 2005. The DNA-binding domain can be engineered...editors, new amino acid recognition sites had to be designed each time we wanted to target new genomic sites... in genome editing is prime editing, which was designed in 2019. It’s based on a prime editor which is...

  9. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...Feng Zhang’s lab at the Broad Institute and was designed and cloned by Neville Sanjana. This fantastic ...your gRNA of interest to mammalian cells. It was designed to have increased lentiviral titer as compared...

  10. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...is absolutely fascinating to see the living art designed by creative minds with a (I assume) very steady...Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology ...

  12. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ... bring this into your target organism. We have designed a Golden Gate system based on the daily needs ...produced will have different overhangs, they can be designed such that they go back together only one way and...

  13. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...When cancers are treated with drugs designed to hit them right where it hurts, the effects are often ...specific contexts, so he assembled a plasmid kit designed to do exactly that. As described in the journal...

  15. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...Organoid EasyTag workflow combines thoughtfully designed plasmid components with Fluorescence Activated... heavy optimization. Claudia Vickers’s lab has designed novel SL fluorescent biosensors by integrating...

  16. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...without end-point sequencing assays.  This tool was designed by putting together the coding sequence of a mCherry...MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent...

  17. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ... of transcriptional regulator or the number of designed scaffold binding sites. With hundreds of characterized...targeted site. Multiple scaffold RNAs can be designed to allow simultaneous, multidirectional and tunable...

  18. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...beginnings of the field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been...including his zinc sensor and his genetically encoded magnesium sensor, the first of its kind. “I hope by depositing...

  19. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...organization is sent the MTA to sign. Once they've signed and returned the MTA, Addgene can move forward ...plasmid hasn’t shipped because a form needs to be signed. But the truth is, meaningful progress depends ...

  20. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...one is as cheap as 10 cents, making activities designed with FD-CF reactions affordable to many schools...students. In our BioBits kit for example, we’ve designed FD-CF experiments that result in outputs that ...
Showing: 41 - 60 of 293 results