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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 273 results for: phy

Showing: 41 - 60 of 273 results

  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... forms that limit its functionality. To make biophysical and structural studies more feasible, the lab...properties, and is amenable to a wide range of biophysical assays (Kroupova et al., 2024). They were able... Cereblon construct for crystallographic and biophysical studies of protein degraders. Nat Commun., 15...

  2. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ... among organisms belonging to many different phylogenetic groups (Jain and Srivastava 2013).  Recombinant...Paracoccus denitrificans, Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp...Meyer, R., Figurski, D., & Helinski, D. R. (1977). Physical and genetic studies with restriction endonucleases...

  3. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...light, oxygen, and voltage (LOV) domains, phytochrome B (PhyB), and cryptochrome 2 (CRY2) (Zhang & Cui,...study multiple aspects of cellular activity and physiology. However, there are a number of factors that ...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University of Manitoba. ...comparisons to enhance our understanding of receptor physiology and the effects of metabotropic and ionotropic... Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student at the University of Manitoba. ...

  5. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    .... Annual Review of Biophysics, 46, 505–529. https://doi.org/10.1146/annurev-biophys-062215-010822 Nishimasu...from making off-target cuts.   Pro tip! X-ray crystallography cannot capture flexible or disordered regions...

  6. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use this kit ...strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer/producer...

  7. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, but you...

  8. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...and did their best to claim the costume contest trophy. We’ve had teams dressed up as plasmids, Blugene...their jaws into a leaf, and dies as its muscles atrophy. After the ant’s death, the fungal fruiting bodies...

  9. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ... The technique successfully resolved loci with physical map distances of 75 and 2 Mbp, with the calculated...distances correlating with the previously established physical map. In comparing pairs of targets ~2 Mbp apart...

  10. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...Methods paper last year. The key to their bacterial phytochome-derived proteins, iRFP670, iRFP682, iRFP702, ...fluorescent proteins engineered from a bacterial phytochrome. Piatkevich et al (Nat Commun. 2013 Jul 10;4:...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...all-optical electrophysiology A major challenge in the field of all-optical electrophysiology is designing...provided the field with an improved tool for electrophysiology, minus the use of electrodes. The Cohen lab...- a real boon to those trying to understand physiological interactions and functions of their POIs. ...

  12. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ... and physiology in vitro and have emerged as a powerful model for understanding human physiology, development...

  13. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...

  14. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... distancing? Many people are now calling this “physical distancing” since it’s important to stay virtually...message for some people. We should recommend #PhysicalDistancing. Social interactions and #social support ...

  15. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...membrane. Common gram positive bacteria include staphylococci and streptococci. Gram negative bacteria, or...in marine biology and minoring in studio arts photography.  References and resources References Silhavy...

  16. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...researchers did the following: Improve sensor’s photophysical properties such as kinetics, ∆F/F0, SNR, dynamic...membrane trafficking and localization. Improved photophysical properties The improved variants of iGluSnFR3...

  17. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ... because of effects of pH on protein stability (Murphy et al., 2013). However, the pH changes in our solutions...the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning...

  19. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...(Richardson et al 2016) revealed some of the biophysical parameters that can influence the HDR/NHEJ decision...sequence replacement by HDR. Understanding Cas9 biophysics enhances HDR We first measured Cas9 dissociation...
Showing: 41 - 60 of 273 results