27 Hot Plasmids from 2016
Type
Blog Post
Published
Dec. 22, 2016, 3:03 p.m.
...Biol. 2015. PubMed PMID: 25895059
Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312...Science. 2013. PubMed PMID: 24136966
Nyerges, et al. Proc Natl Acad Sci USA. 2016. PubMed PMID: 26884157
...generating protein chains or clusters.
Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used GMAP assembly...engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand...number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions generally...