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We narrowed to 413 results for: vent

Showing: 41 - 60 of 413 results

  1. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...not been well characterized as it is not a common event. What are exosome AAVs? In 2012, Casey Maguire ...these particles have some advantages over the conventionally purified AAV vectors. They found that they ...both in vitro and in vivo when compared to the conventional AAVs (AAV preparations were always normalized...target cell types with a similar specifically as conventionally produced AAV particles. But, exo-AAVs may open...

  2. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...channels to apply for our future events. In addition to this annual event, graduate students in different...passionate about communicating science.  ComSciCon's events have 3 main goals: To help graduate students in...your area here: https://comscicon.com/. All our events are offered free of charge to accepted students...

  3. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...few inventory items offered domestically, but by the end of 2016, we expanded our viral inventory to 25... lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing...

  4. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... lentiviral packaging systems were designed to prevent the formation of replication competent lentivirus...Replication competence can arise from recombination events during viral production and poses a biosafety risk... risk to users. To reduce the risk of such events, newer lentiviral packaging systems divide the viral... thereby increasing the number of recombination events that are required to generate RCL. To overcome ...

  5. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...F1000Research, 2014; Dolan, F100Research, 2016). These events have also started progress towards solutions. The... necessary to determine the best solutions. The event also culminated in a joint American Society for ...science-related fields. In particular, two recent events have the potential to influence both graduate students...many institutions to set postdoc wages. The second event of note is a ruling in August by the National Labor...

  6. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...genome and a cell that has had multiple integration events. In addition, promoter choice can also impact transgene... approach can discriminate multiple integration events, reducing the possibility of underestimating the... are used to quantify the number of integration events. Another drawback to this method can be the absence...of infection (MOI) for predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1. PubMed...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... with enhanced sensitivity for unitary synaptic events By Brian O’Neill The Lin Lab and collaborators ...microscopy to detect action potentials and subthreshold events, including miniature excitatory postsynaptic potentials...While studying mEPSPs has been possible with conventional electrophysiological methods, it has remained... with enhanced sensitivity for unitary synaptic events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...designed small RNA, or guide RNA (gRNA). The gRNA conventionally contains a 20-nucleotide sequence that is complementary...becomes complexed with which variant of Cas9. To circumvent this problem, previous studies have proposed ...orthologs are less well characterized than the conventionally used SpCas9 (derived from the bacterium Streptococcus...gene of interest, Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...tract, dCST= dorsal cortico spinal tract, vCST= ventral cortico spinal tract, dc= decussation of the CST...specimen, A= anterior, P= posterior, D= dorsal, V= ventral Developments in light sheet fluorescence microscopy...less photo-bleaching or photo-damage than in conventional laser scanning microscopy . Since image acquisition...developed the 3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains...

  10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ... functional copy of your plasmid of interest, preventing this issue. The genes and their antibiotics   ...produces beta-lactamase to degrade amp and therefore prevent cell death. One of the cool things about amp is...susceptible to degradation in agar plates. This prevents satellite colonies from forming while effectively...expressing a protein that directly binds to zeocin, preventing it from binding DNA. Zeocin is an all-in-one ...

  11. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...discipline. Getting companies to come to these types of events can be very tough. First I needed to find the right...least read my emails. Then pitching myself and the event was very difficult and somewhat awkward in the beginning...better with repetition. I learned to look at the event from the company’s perspective, focus on the message...professional updates. Whenever I see interesting events, articles and newsletters I make sure to share ...

  12. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos, mesothelioma...treatment As with many forms of cancer, the primary conventional treatments for mesothelioma include surgery ...This drug (known as an angiogenesis inhibitor) prevents the growth of new blood vessels required to sustain...treatment get, there still remains the problem of prevention. Asbestos exists pretty much everywhere, still...

  13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...gRNA target site after the first HDR event, then the second HDR event can’t occur. Lastly, depending on ...the 3’ splice acceptor of the transgene to help prevent the accumulation of such truncated proteins. Examples... donors for two consecutive CRISPR-mediated HDR events which results in the integration of a larger donor...

  14. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...and recombination events). TIDER can screen for specific homology-directed repair events and small edits... Fig. 2: Size screening for large deletion events.   Validating knock-in mutations You’ve introduced...combo and are hoping for homology-directed repair events, or maybe you’re performing a single base pair ...

  15. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...identifies a staggering ~250,000 phosphorylation events in the proteomes of higher mammals (2). How can... the importance of any of these phosphorylation events on the activity of a given protein? Classical methods...cysteine in their proteomes (3). This led to the eventual discovery of a naturally occurring phosphoserine-tRNA...inactive without requisite dual phosphorylation events on its activation loop, but can be made constitutively...

  16. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ... the immune system, notably CD4+ T-cells, and eventually leads to acquired immunodeficiency syndrome (...an idea that became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM to "shock and ...the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al. designed...HIV-1 cleavage allows T-cells to survive, but preventing re-infection would require sustained expression...

  17. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... (Bedbrook & Ausubel, 1976). This recombination event produces a multimeric form of the plasmid with repeating...cells compared to monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to... multimerization, and in some cases completely prevent the formation of plasmid multimers (Bedbrook & ...overgrowing the bacterial culture Another way to prevent plasmid multimerization is to avoid growing the...

  18. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...bind to the operator. The bound repressor then prevents transcription from occurring, which means that...to the tetracycline operator sequences (TetO), preventing transcription. In the presence of tetracycline...binds tTA, resulting in a confirmation change that prevents binding to the promoter and turning transcription...binds cTA, resulting in a confirmation change that prevents binding to the promoter and turning transcription...

  19. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ..., and publishing. The livestream video from the event can be found below along with a description of the...issues and their potential solutions. Prior to this event, I gave my own talk on reproducibility at Addgene... this booklet (which was also handed out at the event). Program 0 - 1:24 - Intro 1:24 - 29:31 - Reproducibility...recognizing that we’re biased and taking steps to prevent our biases and goals from effecting our experiments...

  20. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...Nano-Lanterns including monitoring multiple cellular events such as the dynamics of subcellular structures ...NanoLuc® luciferase Many intracellular sensors of events like calcium release have been made using fluorescent...employing optogenetic regulators to initiate the event being monitored. Optogenetic regulators respond ...intracellular calcium and is useful for direct imaging of events in cells and brain explants. This construct is ...
Showing: 41 - 60 of 413 results