We narrowed to 740 results for: NSI

...for the user. This approach serves to maintain consistency with published screen interpretations and allows...curated CRISPR screen data are also available via comprehensive pre-compiled downloads and via a full-featured...are fully open access and available without any licensing restrictions.    As CRISPR technology continues...

... these techniques and is a multi-step process consisting of digestion, purification, ligation, and transformation..., TOPO cloning, one step cloning, and overlap extension PCR. Although each type of cloning has its advantages...combine the products and digest with methylation sensitive restriction enzyme DpnI to remove the templates...

...put on typical middle age weight and “exercise” consisted of taking walks in the mall. I took action, joined...patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family... to raising funds and awareness of FOP. Please consider running with us or making a donation via the link...

...labs. Disposable plastic materials are fairly inexpensive in this country and we are quick to throw out... gel on a large glass slide using only surface tension.  It's a bit tricky to pour the right amount so...plastic and solder this thing together.  One of the downsides—there was no cover so you had to be careful not...

...at our graphics and design editors. How about condensing a week’s worth of the world’s scientific discoveries...like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance of copy...in the science communications world, you might consider an internship or a short program to build skills...

...markers and replication origins, but there is extensive heterogeneity in the feature sequences due to ...control elements in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to...reliable feature annotations. Even with these extensive efforts, such an algorithm has limitations. For...

...how Protein A and Protein B interact. You read extensive research on the two proteins and come up with ...antibody against Protein B. The antibodies are both extensively published, knockout validated, and work in your...with human antibody sequences.  Additional considerations Although isotype conversion is routinely used...

...cells. Until now, tools for doing this were labor-intensive and required using manipulations such as injection...sites in the construct to different lengths, the density of cells that are labeled can be altered to dense...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...

...a relatively pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super...lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for studying...

...proteins in vivo. These probes however can be expensive to make, hard to deliver into living systems, ..., and often do not fold properly once inside cells.  Developing the HA frankenbody To combat the issues...found that the HA frankenbody is effective in sensitive cells such as living neurons, and can be fused...