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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 817 results for: nin

Showing: 641 - 660 of 817 results

  1. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...improve the frequency of HDR over non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the...

  2. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...caught on quickly in the scientific community, turning slightly under ten requests a day in 2017 to over...repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1 are the...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ... coupled to the bacterial host's cell cycle, maintaining a stable concentration of plasmid. Similar to...levels low even if plasmid copy numbers are high, restraining further replication.   Figure 3: Comparison...

  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...their CROPseq method for optical pooled CRISPR screening, Paul Blainey’s lab has developed a multiplexing...resistant to viral transduction. Through in vivo screening of human promoters, the Zhang Lab found a short...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...work, a video abstract may be a useful tool for turning video views into online article downloads – especially...  “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...

  7. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ... gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter (Figure 2). The...transient leaf transformations are a great help in screening candidate genes and speeding up research. Certainly...

  8. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...are still many more features to consider when designing a full gene. A non-exhaustive list includes: ...interest codon optimized by ordering plasmids containing them from Addgene. If a plasmid at Addgene contains...

  9. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...that encode 20 amino acids and three stop codons, meaning that the same amino acid can be encoded by more...synonymous codons) may actually be critical for determining the rate of translation. The ribosome may slow...

  10. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...110.18 (2013). 7306-11. Pubmed PMID: 23589850. 9. Cronin, J., Zhang, X.Y., Reiser, J. “Altering the tropism...16101513. PubMed Central PMCID: PMC1368960. 10. Denning, W., Das, S., Guo, S., Xu, J., Kappes, J.C., Hel...

  11. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...studies. These parameters are less important when designing a biosensor, as the distance between the two fluorophores...many measurement methods as feasible when first beginning to establish the FRET methodology for a given ...

  13. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...effective brightness reflects the concentration of BV-containing molecules in a cell and depends on the efficiency...Biol 24, 758-766 e753 (2017). Baloban, M. et al. Designing brighter near-infrared fluorescent proteins: insights...

  14. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...before we even knew that DNA was genetic material (Jennings, 2011).   The fruit fly makes a great model organism...PMID: 23594743. PubMed Central PMCID: PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology...

  15. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...specific elution of biotinylated proteins. For help determining which biotinylation reagent to use check out ...Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules on surfaces.” Journal...

  16. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...autofluorescence’ include riboflavin in the medium or flavin containing proteins in mammalian cells. Generally, autofluorescence...  References 1. Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane...

  17. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...light, this channel activates neuronal activity by opening and allowing positively charged ions to rapidly...You’ll need to test for these differences when designing your experiment.  Finding the right information...

  18. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...recombination for every lot of AAV vector we produce containing a DIO/FLEx cassette using next-generation sequencing...thoroughly tested in the researcher's system before beginning large scale experiments. This will help to ensure...

  19. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    .... For example, piggyBac and Tol2 are best for screening promoters and enhancers since they are biased ...guided transposons in bacteria sets the stage for combining the super powers of CRISPR and transposons to ...
Showing: 641 - 660 of 817 results