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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 839 results for: igh

Showing: 681 - 700 of 839 results

  1. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...videos. The search function in the manual just highlights what the user searches, which is not very helpful...

  2. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...out of the incubator because I’d had a baby that night, all he said was “OK” and hung up on me). I went... 25 years since our last contact, but we were delighted to be reconnected.  Science can be a small community...

  3. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...next steps (see “action items” below). Get the right people in the room There are few experiences more...informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When no discussion...

  4. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...bandwidth to become an expert) the end result is high quality and the entire process is completed more... What types of issues do you want to know about right away? Provide adequate support. The manager must...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ... and Andrew Hempstead. What do smell, taste and sight have in common, besides being one of the five senses...Chemogenetics is similar to optogenetics but instead of light, these genetically modified receptors are activated...

  6. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...middle) and extends from the 3' end of the nick (right), occasionally introducing mutations (red nucleotide...EvolvR with a targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard...

  7. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...you that Twitter is a tool worth using, but you might be wondering how to get started. This blog post ...video from Science Media Savvy At this point, you might be be thinking social media could be useful, but...

  8. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...and their functions are more intuitive than you might think. So, if you’ve ever wondered what goes into...polymerase II promoter and terminator regions and are slightly different in sequence. Both LTRs do share common...

  9. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...proteins of interest such as actinin. When tension is high vs. low in the cell the donor and acceptor of a ...on changes to the structure of actinin in low vs. high tension. These systems are powerful tools to measure...

  11. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity... Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression ...

  12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...however, they are different viruses and thus use slightly different isoforms of these packaging components...research to reduce promoter interference and/or drive high expression of a transgene from an internal promoter...

  14. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...mentoring group Find 5-8 colleagues that you think might be interested and willing to commit to the group...same place, a 1-2 hour lunch on a regular schedule might work. If not, perhaps a 3 hour breakfast once a ...

  15. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...mouse models. The dTAG system The dTAG system pairs highly selective small molecule FKBP12F36V degraders (...observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature...

  16. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...amino acids. The use of radioactive materials is highly regulated, requires designated work areas and come...passionate about communicating science to all audiences. Right now, she is a science instructor at The Innovation...

  17. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...microenvironments and what encircles a disease is highly prone to affect it. While tumors may be the stars...surroundings and being shaped by them. Another set of highly influential microenvironment cells are Cancer-Associated...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ....7 kb. Co-infection rates for AAVs are generally high enough for this approach to work, although it’s ...time. Though the time delay until expression is highly dependent on the serotype and target tissue, two...

  19. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...viral vectors were useful for researchers wanting high quality reagents, and we realized we could use the...Addgene’s support, could fill a community need for high-quality service, open data, and increased reproducibility...

  20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ... is biotin-conjugated, streptavidin (which has a high affinity for binding biotin), is conjugated to the...interest. The optical density (OD) - the measure of light absorbed by a solution - of these wells is plotted...
Showing: 681 - 700 of 839 results