We narrowed to 817 results for: nin

1. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

   Type

   Blog Post

   Published

   Sept. 19, 2017, 1:20 p.m.

   ... 2). Induced cell death was confirmed by TUNEL staining.  The video below shows what happens to the zebrafish...

2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   Published

   March 24, 2016, 2:30 p.m.

   ...Cas9 breaks repaired by the non-homologous end-joining (NHEJ) pathway disrupt target gene sequences (thus...

3. Plasmids 101: Mammalian Vectors

   Type

   Blog Post

   Published

   March 25, 2014, 3:15 p.m.

   ... allow for episomal amplification of plasmids containing the viral EBV or SV40 ORIs, respectively. The...

4. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ... So how can you get involved? Keep asking your burning questions and tell us what you want to know more...

5. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...specific subcellular localization peptide. By combining three different localization peptides with four...

6. Plasmids 101: Inducible Promoters

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   Blog Post

   Published

   Jan. 18, 2018, 2:34 p.m.

   ...the activator protein can bind to the promoter, turning it ON and initiating transcription.     Negative...

7. OCaMP: A New Calcium Indicator for Neural Imaging

   Type

   Blog Post

   Published

   Aug. 19, 2025, 1:15 p.m.

   ... Scientists have developed calcium indicators spanning nearly the entire visible spectrum (Figure 1)....

8. Fluorescent Proteins 101: Luciferases

   Type

   Blog Post

   Published

   July 22, 2025, 1:15 p.m.

   ...visualized simultaneously. Complementation systems for examining protein-protein interactions Split luciferases...

9. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...your experiment. You can see from Figure 2 that determining where to place your gate based on the dot plot...

10. GPCRs: How Do They Work and How Do We Study Them?

    Type

    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ...PRESTO-TANGO (parallel receptorome expression and screening via transcriptional output - TANGO) (Kroeze et...

11. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in...

12. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ...than you – meeting scientists at your level of training is always a good idea. Make contact with other...

13. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...subsequent cell divisions, the fraction of cells containing the particle exponentially decreases over time...

14. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    .... PubMed PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine...

15. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...opposed to the opaque (and, admittedly, somewhat meaningless) practice of listing “Submitted” articles in ...

16. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ... the major drawbacks of the indirect method, lengthening an often already lengthy process. (And while ...

17. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...and Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell types, including...

18. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...

19. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...an affinity of 110 µM (Zacharias et al, 2002), meaning that at this concentration 50% of the protein forms...

20. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ...easy to identify: double-membraned structures containing undigested cytoplasmic contents. Autophagosomes...