We narrowed to 821 results for: nin

1. Hot Plasmids - January 2023

   Type

   Blog Post

   Published

   Jan. 31, 2023, 2:15 p.m.

   ...these applications have lagged. Fixation, chemical staining, and light exposure can negatively affect FPs ...

2. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

   Type

   Blog Post

   Published

   June 1, 2021, 1:15 p.m.

   ...beyond COVID-19 One year on, our biggest lesson running the platform has been the realization that there...

3. Early Career Researcher Toolbox: Free Tools for Science Writing

   Type

   Blog Post

   Published

   April 28, 2021, 1:15 p.m.

   ...phrase, such as being cautious, being critical, or defining terms. The phrases in Phrasebank are generic, ...

4. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   Published

   Nov. 20, 2018, 8:17 p.m.

   ...number changes) the original identifier loses its meaning, making it difficult or impossible for researchers...

5. Four Factors that Differentiate the Stem Cell Field

   Type

   Blog Post

   Published

   Sept. 28, 2016, 2:35 p.m.

   ...but they are also the reason this research is happening. Stem cells scientists have certainly not lost...

6. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   Published

   March 24, 2016, 2:30 p.m.

   ...Cas9 breaks repaired by the non-homologous end-joining (NHEJ) pathway disrupt target gene sequences (thus...

7. Plasmids 101: Mammalian Vectors

   Type

   Blog Post

   Published

   March 25, 2014, 3:15 p.m.

   ... allow for episomal amplification of plasmids containing the viral EBV or SV40 ORIs, respectively. The...

8. OCaMP: A New Calcium Indicator for Neural Imaging

   Type

   Blog Post

   Published

   Aug. 19, 2025, 1:15 p.m.

   ... Scientists have developed calcium indicators spanning nearly the entire visible spectrum (Figure 1)....

9. Fluorescent Proteins 101: Luciferases

   Type

   Blog Post

   Published

   July 22, 2025, 1:15 p.m.

   ...visualized simultaneously. Complementation systems for examining protein-protein interactions Split luciferases...

10. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...the activator protein can bind to the promoter, turning it ON and initiating transcription.     Negative...

11. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    Published

    Aug. 20, 2024, 1:15 p.m.

    ...your experiment. You can see from Figure 2 that determining where to place your gate based on the dot plot...

12. GPCRs: How Do They Work and How Do We Study Them?

    Type

    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ...PRESTO-TANGO (parallel receptorome expression and screening via transcriptional output - TANGO) (Kroeze et...

13. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ... So how can you get involved? Keep asking your burning questions and tell us what you want to know more...

14. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...specific subcellular localization peptide. By combining three different localization peptides with four...

15. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in...

16. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ...than you – meeting scientists at your level of training is always a good idea. Make contact with other...

17. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...

18. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...an affinity of 110 µM (Zacharias et al, 2002), meaning that at this concentration 50% of the protein forms...

19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ...easy to identify: double-membraned structures containing undigested cytoplasmic contents. Autophagosomes...

20. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...CRISPRainbow paper, Ma et al. engineered gRNA scaffolds containing two hairpin sequences, which recruit fluorescent...