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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 293 results for: gne

Showing: 61 - 80 of 293 results

  1. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...a disease in a model system. TF screens can be designed to identify custom TF programs to acutely initiate...predicted differentiation), and 15/17 morphologically aligned. Documentation of the network that initiates biological...

  5. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...editing, researchers simply need to express a gRNA designed to direct Cas9 to their target sequence of choice... double-strand break. A “gRNA” is a researcher-designed hybrid of the tracrRNA and the crRNA. The “direct...

  8. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... Own your time When I was an undergrad, I was assigned a paper to give as a journal club to my molecular...the student presenting right before me had been assigned a structure paper with two single-panel figures...

  9. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...also known as RNAi, are short, dsRNA molecules designed to degrade mRNA transcripts. RNAi activates the...sequence is found (typically RNA that the RNAi is designed against), RISC can repress translation and/or ...

  10. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ... RNA toehold switch, a RNA hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream...components for gene disruption or CRISPRi. While designed to study the bee microbiome, these broad-host-...

  12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...showing the potential for broad use. This kit is designed for use with acceptor vectors from the MoClo Toolkit...tracing Article contributed by Erin Sanders CreLite, designed by the Eisenhoffer lab, is a new tool that increases...

  14. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...and PE5b: In this approach, the nicking sgRNA is designed to bind only after the edit is installed. This...approach. Figure 7: Nicking sgRNAs can be designed to target edited DNA, so that nicking only occurs...

  15. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... Fragmid is a modular vector assembly process designed for a quick, plug-and-play approach to cloning...sequence. This means that each component can be designed with an overhang specific to the type of module...

  16. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...created the sensor that you need. FRET biosensors designed to measure specific small biomolecules or gene...biosensor, Cameleon (Miyawaki et al., 1997), was designed to measure intracellular calcium and published...

  17. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...forever. microPublications are peer-reviewed and assigned a DOI that can be referenced. In addition the ...continuously curated as we continue to receive plasmids designed for the worm so check back frequently for what...

  18. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...towards solutions. The 2015 Boston FoR meeting was designed to explore areas where further data is necessary...development and implementation of new strategies designed to cope with current increases. These two rulings...

  19. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...individual sequencing reads (~150 bp each) are aligned to the reference sequence of the plasmid used to...nucleotide collection database, and it may also be aligned to plasmids in our inventory containing the suspected...

  20. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...Zimmermann et al., 2020). This selection platform was designed so that any standard lab can quickly select for...strongly to your target protein. The Seeger lab designed 3 different mRNA sybody libraries for use in the...
Showing: 61 - 80 of 293 results