Plasmids 101: Restriction Cloning
Type
Blog Post
Published
Feb. 18, 2016, 3:42 p.m.
...500nm) Light
0.1
Crystal Violet
Pre and Post
Visible Light
100
Methylene Blue
Post
Visible Light
100
... and Post
Blue or UV Light
0.5
GelRed
Pre and Post (Post recommended)
UV Light
0.1
GelGreen
Pre and...you to visualize your DNA using UV light – please note that UV light can damage DNA and that proper personal...are having trouble getting colonies, you might want to use higher competency cells. Additionally, if your...bacteria; add chemicals to precipitate out the high molecular weight genomic DNA; filter the remaining plasmid...100
Ethidium Bromide
Pre and Post
UV Light
0.5
For more information on these stains see the Bitesize...final product is going to be very large (>10kb) you might want to use electro-competent cells instead of the...