We narrowed to 273 results for: phy
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TypeBlog PostPublishedJuly 6, 2023, 1 p.m....of applications within chemistry, biology, and physics, in this blog we will focus on those which can ...monitor the fundamental cellular building blocks. Physiological factors Unlike the previously described sensors...
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Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....degradation. Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective...can be used to carry out any biochemical and biophysical study involving protein stability or binding ... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....biology at Virginia Tech, and earned a Ph.D. in Biophysics from UC Davis in 2007. Nikolai trained as a post-doc...ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute ... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....low-throughput methods such as patch-clamp electrophysiology, a technique that’s used to measure electric...selected for characterization with patch clamp electrophysiology. All thirty were functional and their measured... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....present in the cell, it can also introduce non-physiological RNA-protein interactions due to sample processing...t be used to study endogenous RNAs at their physiological expression levels; however, knock-in of BoxB... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m.... ligase, is the basis of TRIM-away. In normal physiology, TRIM21 recruits ubiquitin and the proteasome...enable future researchers to better simulate physiological gene expression regimes that cannot be easily... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m.... The technique successfully resolved loci with physical map distances of 2 and 75 Mbp, with the calculated...distances correlating with the previously established physical map. In comparing pairs of targets ~2 Mbp apart... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m.... brain. But studying synaptic connections and physiology is particularly challenging as they are great...target the synaptic scaffolding proteins PSD95 and gephyrin to label excitatory and inhibitory synapses, respectively... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....fluorescence complementation analysis under physiological conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques... -
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....encoded in the transfer plasmid, the component that physically integrates into the host genome, the virus is...caution as they are integrating viruses – they physically deposit their cargo into the host’s DNA. These... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m....Biosciences (UCIBIO) focused on understanding the physiological and pathological roles of human cell glycans.... Her main work focuses on unraveling the pathophysiological role of glycans. Follow her on Facebook: ... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m....bioproduction of therapeutic methylxanthine drugs like theophylline. To facilitate future work with this goal, the...peak of 519 nm. These proteins have improved photophysical properties and can be used in quenching, FRET... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....art with colorful microbes? Try your hand at photography and participate in a science photo competition...Indonesia. Since then, I have tried many things: photography, making videos, presenting in pubs and at science... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m.... tension microscopy (MTM), which could monitor physical stresses or even apply forces (Gayrard and Borghi... Biosensors and Intravital FRET Microscopy." Biophysical Journal 111.6 (2016): 1103-1111. PubMed PMID:... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....biology at Virginia Tech, and earned a Ph.D. in Biophysics from UC Davis in 2007. Nikolai trained as a post-doc...ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute ... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m.... do things like translate perceived signals to physical actions. Understanding neuronal pathways and the...membrane, and this voltage is particularly physiologically relevant in neurons, myocytes, and endocrine... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m.... molecular biology-grade agarose containing physiological concentrations of salts and glucose. If we circled... terms of growth rates, morphology or other physiological properties. An advantage of this technique is... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....biological microscopy." American Journal of Physiology-Cell Physiology 300.4 (2011): C723-C742. PubMed PMID:... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....requires incubation at 55 °C - a dangerous, non-physiological temperature for mammalian cells and one which...things (101 lessons on Spanish, C++, guitar, photography) and often dreams of a sunny day in Edinburgh... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....example), as this can also be perturbed by supraphysiological expression levels. BiFC quantification This... amenable to studying PPIs under relatively physiological conditions when compared to FRET. However, it...