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We narrowed to 413 results for: vent

Showing: 61 - 80 of 413 results

  1. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... (FSF) sequence prior to an ATG start codon to prevent transcription in cells where the recombinase was...between two lox2272 sites in the same orientation preventing the transgene from reverting. Figure created ...problem of DIO/FLEx vectors, the cause of these events, until recently, was not known. A recent paper ...2020) it was determined that the optimal titer to prevent off-target expression of the sensitive FLEx-split...

  2. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...-stimulate mammalian neurons and alter synaptic events: a new technology was born, and the term “optogenetics...antisense orientation with respect to the promoter to prevent its expression, and is flanked (“floxed”) by two...they targeted the dopamine (DA) neurons of the ventral tegmental area (VTA) of the brain. In particular...Optogenetic activation of dopamine neurons in the ventral tegmental area induces reanimation from general...

  3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    .... elegans approaches rapidly enrich for editing events without the need for any selective marker to remain...the awesome power of genetic selection. Knock-in events tend to be rare, and the challenge with any experiment...detailed approaches in which selection for an editing event that produces a visible phenotype enriches for knock-outs...screening 768 F1 animals). Recovering rare editing events in a sea of unedited animals struck me as a “needle...

  4. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...modification with an ssDNA substrate.  Gam: Gam prevents both the endogenous RecBCD and SbcCD nucleases...repressor gene, CI857, is used.  This mutant repressor prevents expression of the recombination genes at low temperatures...modify chromosomal genes. After the initial editing event, the defective prophage can be removed from the ...host E. coli by a second lambda red recombination event. Alternatively, if the modified allele is selectable...

  5. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...Existing therapies and effects on immune system Conventional cancer therapies include radiation therapy (...surgical removal of the tumor. However, these conventional therapies produce lasting side effects, damage...system, the therapeutic ability of even these conventional antitumor treatment strategies can be enhanced...immune cells harvested from patients and thereby prevent cancer cells from using this receptor to evade ...

  6. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...Auditorium, Boston, MA Register on Eventbrite (This is a free event) Read more Science Career Development...and meeting new people by working together on the event planning and execution. I am seeing many similar...

  7. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...information back. No team has all the answers – reinventing the wheel is inefficient. An effective team regularly...Solicit feedback regularly and often A one-time event doesn't work to get people sharing.   Frequently...in meetings, via email, at formal communications events or at informal gatherings, like at lunch. Many ...

  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... of recording permanent "memories" of molecular events. These memories would be recorded in the cell's...the relationships between pH changes and cellular events. To overcome the issues with fluorescent pH sensors... tools for spatial and temporal control of cell events Three years ago, Brian Kuhlman’s lab designed photoswitchable...between the truncated gene and the promoter to prevent leaky expression of this resistance. Unlike previous...avenues through which one can examine molecular events with exceptional precision in a native context.... transcription factors and chromatin remodeling events, demonstrating the utility of the pXPG construct...

  9. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...few months. You can also browse our entire AAV inventory. Our new AAVs include: Optogenetics AAV Control...in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype...

  10. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...she said’. We spoke about it for a while and he eventually calmed down, apologised and admitted he’d been...pressure recently which had affected his judgement. Eventually, we published the paper in a high-impact journal... dive as deep into your research at an outreach event as you might in a scientific paper. Build lots of...

  11. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...classes, workshops, book clubs, seminars, and social events. Building a community science lab In the Fall...it. We started out by holding a few journal club events and soon realized that it was easy to find people...facebook. If you are local, join us for our next event on meetup.com! Many thanks to our guest blogger...

  12. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ... is completely plausible that scientists will eventually have available genome editing techniques that...report on the ethics of “Novel techniques for the prevention of mitochondrial DNA disorders.” This document...public views on emerging IVF techniques designed to prevent the transmission of mitochondrial disease;” The...

  13. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ... longer pieces of synthetic DNA, as well as the advent of extremely fast, cheap and accurate sequencing...high-throughput next generation (NGS) sequencing have prevented laboratories from using these methods for routine...quantities. This variation is problematic for conventional NGS library prep methods because the yield or...

  14. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... type of memory which includes autobiographical events (see Jin & Maren, 2015). Directional flow of information... Viral vectors and the nervous system With the advent of viral vector technology, new methods have been...neuronal connection via a single synaptic step, thus preventing further widespread expansion of RABV. This improvement...at Addgene). Its efficiency is comparable to conventional dye-based tracing while also having the added...

  15. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ... a decidedly uncomfortable 45°C). Their more adventurous cousins are the extremophiles - the legends who...the industry and brainstorm with each other. The event also featured a seminar on “Tools to increase scientific...are such endeavors a welcome changeover from conventional academic settings, these are an eye-opening ...

  16. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ... pR, preventing expression of a reporter gene. When light is present, YFI is inactive, preventing repressor...inactive because a bound repressor protein actively prevents transcription. Once an inducer binds the repressor...

  17. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...from a defined pathway or reaction to a complex event such as cancer or even aging. For both a screen ...treatment with small molecules/drugs. With the advent of CRISPR, genome-wide knockout screens are possible...manipulations are assessed, rather than all manipulation events. To put it another way, only cells or organisms...

  18. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...team and software engineers to update our Lab Inventory Management System (LIMS) to accommodate our new... other backgrounds at conferences or networking events. And if you’re a wet lab scientist who works frequently... some of the hurdles I needed to overcome.  I eventually pinpointed and (mostly) solved two main problems...

  19. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...increase stability.  Nature has evolved ways to prevent degradation of important cellular RNA molecules...nuclease-resistant, resulting in less off-target events. While both have similar advantages, bridged nucleic...CRISPR-Cas9 system can initiate targeted editing events. Both technologies utilize chemical modifications...

  20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...proteins should be useful for monitoring cellular events occurring at varied time scales.  Table 1: Monomeric... cellular structure and a particular biological event (trafficking to a subcellular location, start of...find use in studies of animal development where events like the patterning of nascent tissues and the ...
Showing: 61 - 80 of 413 results