We narrowed to 839 results for: igh
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TypeBlog PostPublishedMarch 29, 2016, 2:30 p.m....amplify your insert DNA. It is important to use a high fidelity polymerase to minimize mutations. The fidelity...
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Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....Then ask for their honest opinions -- this part might hurt, but if you pitch to enough people, you will... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m.... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....Watson-Crick base pairing, allowing for rational, straightforward design. Since the technique relies on base ... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....knowledge to impart. An undergraduate can mentor a high school student, a graduate student can mentor an... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m.... jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting state and its calcium-bound... -
Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....business consulting, mathematics, and more. That’s right, we have non-traditional scientists working in our... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....and, depending upon who your challenger was, you might have to drastically alter your strategy. I realized... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....back actual magic (which had been lost for an age) fight in the Napoleonic Wars and against something even... -
Advice for Scientists Starting a Lab
TypeBlog PostPublishedApril 29, 2014, 1:43 p.m.... Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....transfection into mammalian cells is fairly straightforward and the resultant cells can either express ... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m.... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....neuroscience with ever more efficient tools for light mediated neuronal control. The advantages of optogenetics... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled ... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....In many cases, CRISPR may be more efficient. You might often start with CRISPR/Cas9, but TALENs do have... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....is important to know that the student presenting right before me had been assigned a structure paper with... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice thing about... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...