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...Antibody dependent cellular cytotoxicity (ADCC) is a slightly more direct method than opsonization. In ADCC,...

...consider. Antibiotic selection Selection is straightforward in cases where the inserted DNA includes an...

...faculty and introducing the kits to clients who might initially be doubtful of online science lab classes...

...productivity by providing world-wide access to curated, high-quality data and reagents Addgene makes it easy ...

...’s not our only job - we strive to also give you high-quality educational resources to help you do better...

...recommended names and synonyms will all get you to the right entry.) You can look up the cell lines you are currently...

...an opportune time or go all out with constitutive high level expression? Finally, you can add bells and...

...of troubleshooting failed experiments. Pick the right strain While common E. coli strains like DH5-alpha...

...-enveloped virus (left) and an enveloped virus (right). Created using biorender.com   If you’re new to...

...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields are...

... the same protein, their gene sequence may have slight or large variations depending on how closely related...

...fertilizer, seeds and a growth space with fluorescent lights. Other experiments require only seeds, petri dishes...

...biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions...

...elements Coding sequence features are relatively straightforward to define, but for control elements such as...

...amplify your insert DNA. It is important to use a high fidelity polymerase to minimize mutations. The fidelity...

...Then ask for their honest opinions -- this part might hurt, but if you pitch to enough people, you will...

... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds...

...Watson-Crick base pairing, allowing for rational, straightforward design. Since the technique relies on base ...

...knowledge to impart. An undergraduate can mentor a high school student, a graduate student can mentor an...

...and, depending upon who your challenger was, you might have to drastically alter your strategy. I realized...