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We narrowed to 468 results for: SEC

Showing: 81 - 100 of 468 results

  1. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    .... This app also has a great educational section. This section holds the Gibco Cell culture manual and ...favorite lab app, please share it in the comments section below! Many thanks to our guest blogger Sean Stacey...

  2. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ... the substrate coelenterazine and is likely the second most commonly used luciferase. Other organisms ...Peak emission (nm) Size (kDa) Substrate Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455...cell membrane and deliver the substrate; however, secreted luciferase or the use of alternative substrate...

  3. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...   Once you get your exciting new antibody, the second thing you’ll want to do is store it away safely...kit, and is readily available on any number of secondary antibodies. And look - if it ain’t broke, don’...fluorophore strength with protein abundance.    Second, you’ll need to consider spectral overlap. Fluorophores...

  4. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...overexpression and knockdown. Addgene also has a number of insect vectors to consider. An easy way to see what is...available is to browse Addgene’s Collection under the insect expression system or by filtering by fly as species...organs of interest, such as the intestines, can be dissected at various times throughout the lifespan of the...

  5. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... plasmid page under the "Resource Information" section. Analyze your plasmid sequence For more immediate...also click one location in the plasmid and then a second location to select the sequence between the two...what you think about the updates in the comments section below and keep checking in at blog.addgene.org ...

  6. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular Cytology (University of Amsterdam)...

  7. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...business competitions. When the team was unable to secure enough money to sustain a startup, he went to work... the data must be reproducible by someone else. Second, "an entrepreneur must be OK with the constant ...or product? Please let us know in the comments section! And read on for more great information about starting...

  8. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ..., my mind went completely blank. After several seconds of truly panicked silence, I opened my mouth to... rousing success–the response was a full sixty seconds of silence–but it was thorough and coherent.  To...nerve to do what I did. Until that panicked, split-second decision, I had always thought I had an obligation...

  9. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...present. One common example is using pre-adsorbed secondary antibodies (which recognize other antibodies) ...this can increase background staining (see next section), it may be advantageous if you are trying to detect...useful for certain research applications. Many secondary antibodies are polyclonal, as their function is...

  10. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular Cytology (University of Amsterdam)...

  11. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...PHP.eB compared to the existing ChR2(H134R).  In the second experiment, ChRger2 was delivered and activated...-invasive methods to the neurons in the right secondary motor cortex (M2). Since M2 is located on the ...lab could thin the mice’s skull near the M2 and secure a fiber optic cannula to the skull, leaving the...

  12. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...antibody, a primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can ...can bind to a single primary antibody. The secondary antibody is conjugated to an enzyme for visualization...

  13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to look for... that cite the background, methods, or results section is also displayed. Saving papers Papers can be...that contain these terms. You can also create intersects in your feed which means only papers than contain...

  14. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...and a line about what I hope to discover. This section may make the difference in whether you get funding... the successful use of your preliminary data to secure further funding. Make it clear that you intend ...your head up! It’s very likely that at first (and second) you won’t succeed, but you must try, try again...

  15. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...which does not have primary and only contains secondary antibodies, allowing us to only image autofluorescence...independent screening”, where they look at the same section and determine the antibody’s reliability through...neuroanatomy without the fear of referencing a wrong section. The antibody screening and development of a focused...

  16. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...transcript.   Figure 1: A predicted conserved secondary structure and sequence conserved Rho-independent...contains enzymes that cut RNA not protected by secondary structure. It is thought that poly(A)s are used...example, relies on a specific sequence and RNA secondary structure to induce transcript cleavage, similar...

  17. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...V1, with secondary peaks in V3 and V4, whereas BV711 peaks in the V13 channel, with secondary peaks in...101:Selecting the Right AntibodyAntibodies 101: Secondary AntibodiesAntibodies 101: Buffers, Storage, and...

  18. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ... to its final destination. As an example, most secreted proteins are produced in the endoplasmid reticulum...golgi and then transported in vesicles via the secretory pathway to their subcellular or extracellular ...Edition. 3.  CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka...

  20. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...originally named orthogonal-plane fluorescence optical sectioning (OPFOS) (2). Arne Voie, David Burns and Francis...confocal microscopy In confocal microscopes, optical sectioning of a specimen is based on discriminating the ...semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire one image, thereby significantly ...
Showing: 81 - 100 of 468 results