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We narrowed to 385 results for: abo.5

Showing: 81 - 100 of 385 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...10983732. 5. Gene Therapy Net Lentiviral Vectors Page Additional Resources on the Addgene Blog 5 Tips for...vector genome which was composed of the wild-type 5’ and 3’ LTRs, the ψ sequence, a part of the env gene... from a separate plasmid, and the promoter of the 5’LTR has been deleted to reduce its activity. A CMV...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...even your own! You should therefore be cautious about inserting potentially harmful genes into a transfer...

  2. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...building relationships and thinking about future career options. 5. Write a grant - even if you don’t ...mentoring group (see above). However, now that we are all so good with video calls, how about trying your hand...blog or podcast, initiated and maintained a collaborative project, served on the board of a professional...appointments with people all over the world. Think about contacting alumni of your lab or university or use...suggest you speak with. See this post that’s all about informational interviews, for more details on how...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... Cell 18, no. 5 (2016): 573-86. PubMed PMID: 27152442. PubMed Central PMCID: 4871596.  5. González, Federico...phosphatase treatment, substituting cytidine with 5-methylcytidine, and/or substituting uridine with pseudouridine...by the piggyBac transposon." Nature Methods 6, no. 5 (2009): 363-69. PubMed PMID: 19337237. PubMed Central...PubMed PMID: 20952534. PubMed Central PMCID: 2956203 5. Robinton, Daisy A., and George Q. Daley. "The promise...methods do you have experience using? Let us know about your iPSCs experiences in the comments below! References...Additional Resources on the Addgene Blog Learn about Methods to Reprogram Peripheral Blood Cells Check...

  4. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...This is the third in a 5 part series on Management for scientists. Feedback is help and it should result...build it into your routines. This is the third in a 5 part series on Management for Scientists. Subscribe...employee about what they do well and where they need to improve, and they’re also clear about how the ...enough to tell them about areas they need to improve in. If a manager has concerns about an employee and ...language to talk about real performance issues. Watch this little video to learn more about indirect language...Medical School researcher Connie Cepko to learn about her management style. Formalizing the feedback session...informal feedback is a very effective way to bring about change in behaviors and habits in a nonthreatening...

  5. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...Bleomycin glycopeptide Induces DNA breaks Bactericidal 5-100 ug/mL Erythromycin macrolide Blocks 50S ribosomal...subunit; inhibits peptidyl translocation Bacteriostatic 5-25 ug/mL in EtOH *In prokaryotes.     **Dissolve...molecular biology, yet scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation... sterile filter unless otherwise specified. The above table lists some antibiotics commonly found in the...or light – do not add them to media hotter than about 55oC and store plates/stocks wrapped in foil if ...

  6. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...outperform Cas9 in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather...if NgAgo preferred certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences...impaired NgAgo activity, with three mismatches abolishing activity. One-guide faithful NgAgo guide loading... how it’s working in the comments section! Read About NgAgo User's First Impressions Here References ... Additional Resources on the Addgene Blog Read About Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants...

  7. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...consistent 5% positive error across the plate is fine; a 5% positive error on one side and a 5% negative...  Probe should have a melting temperature 5-10 degrees above the primer and be at least 25 bp away from... Probe is on a hairpin structure included in the 5’ primer Yes Yes. Agarose gel  LUX fluorogenic...

  8. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...Several behavioral assays can be done on zebrafish at 5 days post fertilization, including escape behavior...behaviors, the researchers measured the tail movements of 5 day old fish. The larvae were immobilized in agar ... posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use...

  9. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... This is the first in a 5 part series on Management for scientists.  “I'm slowly becoming a convert ...carrot, and not the stick.     This is the first in a 5 part series on Introduction to Managing People for...the same as not being a bad manager (see cartoon above – what type of bad manager have you had?). You can...Managing scientists I should add a few special words about managing scientists because, let’s face it, we are...

  10. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...a free platform for sharing short, 3 to 5 minute videos about research. Videos can be shared by research... for communicating research. Short videos of 3 to 5 minutes provide more than enough time for researchers...measure the impact of their contribution. It offers a 5-step process and several examples for creating effective...learn about how researchers are using “big data” to look at Lyme Disease in new ways. Or learn about the...ot­her disciplines, are even less likely to read about our work. We therefore must discover new ways to.... Only on occasion do we then have time to read about research from other disciplines, or from other countries...communication through video     Video abstracts about research, especially published research, have grown...

  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...   The Mello lab also reported that modifying the 5’ modification of oligos used to generate linear dsDNA... animal.  PAM sequence considerations For changes 5’ to the PAM, sequences corresponding to the same strand... using two Cas9 targets. An orientation where the 5’ ends of the PAMs are facing outwards, ensuring the...Edraki A, Watts JK, Sontheimer EJ, Mello CC (2018) 5′ Modifications Improve Potency and Efficacy of DNA...learn more about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design...interest, I encountered low efficiency (0.13%) and laborious handling (screening 768 F1 animals). Recovering...

  12. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...of culture Approximate DNA yield** Miniprep 1–5 mL 5–50 µg Midiprep 25–100 mL 20–200 µg Maxiprep...for example. These kits are designed to handle ~1–5 mL of liquid culture. Using a higher volume can affect...µg Megaprep 0.5–2.5 L 1.5–2.5 mg Gigaprep 2.5–5 L 7.5–10 mg *Can vary from kit to kit, check the...columns we’ve been talking about!) are a significant source of waste in laboratories. To help combat this, ...dependent on many different factors as we detailed above, so be sure to pick what kit works best for you!...

  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...Cellular Biology 5(11): 3274–79. PubMed PMID: 2427927. PubMed Central PMCID: PMC369144. 5. Kranz, Andrea...Cellular Level in the Mouse.” Nature Biotechnology 21(5): 562–65. PubMed PMID: 12665802. 9. Villa, Katherine... on the Addgene Blog Learn More about FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs...expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and...

  14. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...their Roller phenotype. If SEC is inserted at the 5’ end of a gene, the SEC separates the targeted gene...are then heat shocked to remove the SEC and after 5-6 days of growth, wild-type animals that have undergone...just one injection step! The entire protocol takes about 2-3 weeks but requires less than eight hours worth... this species, namely, the robust HDR mentioned above, an accessible germline, and multiple selectable...modular system that requires very little hands-on labor and could facilitate the tagging of every protein...

  15. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...the protein with enterokinase, which recognizes the 5 amino acids on the C terminal of the tag. Of the most... most common tags, FLAG is the most charged, with 5 negatively charged and 2 positively charged amino ...Tags have a lot of major benefits – as discussed above – but there are a few instances when they should... hindrances, and destabilization can be brought about by the tag so it’s important to validate tagged ...

  16. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...AAV9, AAVrg pAAV-hSyn-DIO-mCherry 37825 AAV1, 2, 5, 8, 9 (20 µL size serotype testing) pAAV-CAG-GFP ...size serotype testing) pAAV-CAG-GFP 50465 AAV1, 2, 5, rg (20 µL size serotype testing) pAAV-hSyn-EGFP ...vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825  AAVrg...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  17. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...expected to be >12 kb. However if it recombines at the 5’ and 3’ LTR regions, it splits into two smaller plasmids...recombined vector backbone.   Ready, set, grow! Our 5 Tips for Troubleshooting Viral Transductions will ...molecular biology and viral vector protocols Learn about the common lab E. coli strains Find tips for viral...

  18. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... codons/second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein...Emi1, showing that ~80% of transcripts with a long 5’ UTR were translationally silent. Both of these methods...Additional Resources the Addgene Blog Learn More about SunTag Catch up on Your GFP Background Choose the...

  19. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...Coli Synthetic Biology.” ACS Synthetic Biology, vol. 5, no. 10, American Chemical Society (ACS), 2 May 2016...Traditional Biology.” ACS Synthetic Biology, vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015....” PLOS ONE, edited by Alvaro Galli, vol. 13, no. 5, Public Library of Science (PLoS), 30 May 2018, p.... vol. 8, no. 11, American Chemical Society (ACS), 5 Nov. 2019, pp. 2593–606. Crossref, https://doi.org... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila. ...

  20. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ...editing by DNA adenine base editors. Science Advances, 5(5), eaax5717. https://doi.org/10.1126/sciadv.aax5717...—unintended changes to nearby target bases. Up to 5 bystander adenine bases were edited in cellular tests...Baby KJ” was diagnosed with a rare and dangerous metabolic disease shortly after his birth. Within a seven-month...successful base editing intervention was created. Only about half of babies with KJ’s disorder survive long enough...effort was only possible because of a massive collaboration between research hospitals, universities, private...an astounding feat of public investment and collaborative scientific effort. As we like to say at Addgene...activity of adenine base editors The first ABE was about 50% efficient in human cells on average, but its...
Showing: 81 - 100 of 385 results