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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 293 results for: gne

Showing: 81 - 100 of 293 results

  2. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...that’s suitable for PCR amplification using pre-designed primers. By using this library to infect an animal...isolate DNA from the infected animal and use the pre-designed primers to determine which version(s) of the capsid...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ... main requirement for IVA cloning is carefully designed primers. The majority of mistakes are due to incorrect... bind at either side of the undesired portion, designed to amplify the rest of the vector sequence. Encode...

  4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...These libraries utilize optimized sgRNAs that were designed based on the rules identified in Doench et al....1,000 non-coding control sgRNAs. This library was designed to improve on-target activity while reducing off-target...

  5. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...-specific primers: Insert-specific primers are designed to anneal to an insert-specific sequence. This...design backbone-specific primers. These primers are designed to anneal to sites that flank the insert site....

  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...Barcoded Tube Plate Freezer Rack Freezer Tubes are assigned a location within a plate that can hold up to ...also barcoded with a 10 digit barcode and then assigned to a location in a metal freezer rack that contains...

  7. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...such techniques can be problematic, however. Well designed aptamers can help reduce such background, thereby...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...

  8. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...from Streptococcus thermophilus and a rationally-designed truncated Cas9. Unfortunately, certain drawbacks...J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL, Desmarais...

  9. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...each week. Our internal software was originally designed to help us work in large formats, such as 96-well...alone! The benefits of containers are they are designed to ‘contain,’ in a complete package, all of the...

  10. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...microbial survival despite exposure to antibiotics designed to kill them or to impede their growth. When we...equipment, etc. Virtually any preventive measure designed to help keep you from getting sick can be helpful...

  11. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...CRISPR/Cas9 system floating around, and many pre-designed plasmids containing these variants ready for purchase...desired edit. Good news! Remember the primers you designed to generate your pCAG-EGXXFP plasmid? They are...

  12. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...other words, your optogenetic experiment should be designed around a behavioral test in which your circuit....Diester, Ilka, et al. "An optogenetic toolbox designed for primates." Nature neuroscience 14.3 (2011)...

  13. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...and some of the most popular commercial strains designed to avoid them. Why do I need an expression strain...Proteins: The most common purification protocols are designed for soluble, cystosolic protein products, but ...

  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... updated Nov 3, 2022 by Susanna Stroik. You’ve designed your gRNA and introduced it into your target cells...your desired edit. This silent mutation should be designed solely for restriction enzyme sensitivity or resistivity...

  16. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...I really brought to the table was empathy, a willingness to listen, and an ability to remember the right... actively runs away from it. That can mean a willingness to make yourself temporarily uncomfortable for...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...mutated clones. Forward and reverse primers are designed to be complementary, but each primer may extend...and allows for the introduction of specifically designed mutations (not limited to frame shift mutations...

  18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...enhance transcriptional activation. Zhang et al. designed multiple gRNAs to target the 5’ long terminal ...prevent reinfection of those cells. Kaminski et al. designed gRNAs to target the HIV-1 5’ and 3’ LTRs and expressed...

  19. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ... light in combination with retinal) variations designed to give researchers flexibility in using optogenetic...precise timing of the perturbation. Other tools are designed to be activated within a narrower band of wavelengths...

  20. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...possible NN pairs in the template. The probes were designed such that each possible pairing resulted in a ... using a capillary tube, probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical...
Showing: 81 - 100 of 293 results