...editing these cells to avoid a negative immune response (e.g., graft vs. host) as well as the introduction...allogenic therapies to prevent a graft vs. host response. Off-target effects are also important safety ... considerations for all CRISPR therapies, but especially concerning when using a systemic approach. Regarding... will not work in a meaningful way. Delivery, especially for systemic approaches, has its own unique set...

... of p53 tetramerization in response to DNA damage controls the p53 response. Gaglia G, Lahav G. Mol Syst...interact in cells and produce background signal (especially for some older split-GFP constructs or certain...functionality or binding of your target proteins, especially if the termini are important for their interaction...

...time moaning about their troubles, Silent or unresponsive clams, who seem to ignore you and your questions...fellows are hesitant about expressing their ideas, especially if their supervisors are difficult people. The...will help you to communicate with confidence earn respect from your supervisor. Whenever possible, discuss... you even when you meet in person (silent or unresponsive clam), you will probably need support from your...

... inactivate specific genes in mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in...-in systems, these systems remain valuable, especially in cases where CRISPR cannot be used (e.g. there... example shown in Figure 1, large sequences corresponding to exons 1 and 3 of the targeted gene have been...physiopathological events to which the chosen promoter responds. You can also use this method to tag an endogenous...

...product is toxic to cells at high concentrations. Despite their advantages, mammalian expressions systems...provides the highest level of protein expression. Despite their ease of use, it is important to note that... access and purification of a desired protein. Despite being a rather nascent technology, plant cells ...and copper inducible PHO5 and CUP1 promoters respectively. Yeast cells are grown in well-defined media...

...signaler. The pro of this system is the kinetic response time from signal input to readable output, which... is particularly powerful for in vivo imaging especially since JFs can cross the blood brain barrier and...molecule is added exogenously to measure a phenotypic response in the form of fluorescence or voltage indicators... to direct your voltage sensor selectively is especially essential. If you want to learn more about how...

...daily tasks, meet cross-team project commitments, respond to any issues raised by team members, and plan ... all the things for which you are officially responsible on paper, and take some time to write down all... have to exercise in order to manage these responsibilities. If you are in charge of completing many ...management and organizational skills. If you are responsible for working with lots of students, managing inventories...

...SARS-CoV-2 toward destabilizing one’s natural immune response to Adenovirus (and/or to its partner, AAV) or ...transient due to the fact that AdV surface proteins (especially fiber’s knob domain, and hexon), as well as other...all recruit strong innate and adaptive immune responses that inactivate viral genomes or otherwise clear...Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector infected cells does not require...

...result can leave scientists under-informed with respect to how different serotypes, promoters, and injection...conducted to improve our Addgene Data Hub, 76% of respondents indicated they wanted to see more negative data...flaws or gaps in their technical ability. But, especially for AAV data, that may not be the case. We can...transfer to the rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(...

... Although we are busy with many other work responsibilities, we make team building activities a priority...don’t make things too complicated for yourself (especially if you are the sole event planner)! If you have...wise lady for many reasons, but this saying is especially true. If your event requires a lot of set up ...at Addgene. In addition to her operational responsibilities, she enjoys organizing team building events...