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We narrowed to 604 results for: REV

Showing: 301 - 350 of 604 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A....number with links to the plasmid pages for your review. We’ll also mail you a deposit kit with instructions...

  2. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can.... Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of Site-Specific Recombinases on ...

  3. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...based methods for viral titration consider using a reverse transcriptase mutant in a separate control reaction...over-inflate results. A packing vector deficient in reverse transcriptase will produce low levels of virus ...

  4. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ... histones, the more compacted it becomes. This prevents the cellular machinery from physically interacting... further reading and viewing The Epigenetics Revolution: How Modern Biology is Rewriting Our Understanding...

  5. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...need to be signed, or manuscripts that need to be reviewed, bring them to the meeting. This will make it ...items. This will give your supervisor a chance to review what you have discussed and add suggestions if ...

  6. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The... less. The list goes on... In this post, we’ve reviewed many of the most popular cloning methods out there...

  7. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...before entering the hood is another great way to prevent contamination. This sounds rather trivial, but ...closed when they aren’t in immediate use. This will prevent spills, but, if any spills do occur, they should...

  8. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used species... in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

  9. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture...proteins at endogenous loci, shedding light on previously unknown protein functions. References Schwartz...

  11. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...These benefits of Cas3 do come at a cost — the previously mentioned issues with expressing all Cascade ...evolutionary classification of CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/...

  12. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... et al., 2021; Kattnig et al., 2016), and some previous attempts at magnetic control of biological systems...exist at all. Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...

  13. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...by encapsulating a single enzyme. Compared with previous research, which required protein compartments ...inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression...

  16. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... those of the pancreas and colon. Despite the prevalence of Ras mutations in a variety of cancers, Ras...different mutant versions of Ras that are most prevalent in human cancers, and synthesizing compounds to...

  17. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... lab, or by a previous group. If the plasmid was made by a modifying a vector previously deposited with...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...large field-of-views (6). For more comprehensive reviews on several other LSFM configurations’ various features...

  20. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity and accuracy...recombinant AAV2 particle aggregation and methods to prevent its occurrence during vector purification and formulation...

  21. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... vector can still occur even in the case of no previous immunity existing for that adenoviral vector (...(2019). Are adenoviruses zoonotic? A systematic review of the evidence. Emerg Microbes Infect, 8(1), 1679...

  22. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...more accessible way to image biological features previously only visible via techniques like super-resolution... disciplines now available. In this post, we’ll review the inspiration behind ExM, how it works, and some...

  24. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are also...cautious during the aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced...

  25. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...large number of honorees to have trained under a previous laureate (Zuckerman, 1977; Ma et al., 2020). Many... to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman in ...

  28. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ... blog. CRISPR-based genome engineering has revolutionized the gene editing field by making experimental...considerably easier, faster, and more efficient than previous methods. Still, generating reliable results from...

  29. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. ...involves insertion of landing sites via Cas9-directed reverse transcriptases, followed bylanding site recognition...

  31. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David...conversion by engineered glycosylase. National Science Review, 10(8), nwad143. https://doi.org/10.1093/nsr/nwad143...

  33. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... you will not be a graduate student or postdoc forever, and you need to be thinking about what comes next... development center, if one exists. Get online! Review company and institution career pages. Make yourself...

  34. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...takes on cases requiring in-depth analysis, data review, in-house testing etc. Technical support roles ...too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting protocols,...

  35. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate...gRNAs into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9...

  36. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...require a PAM, and RNA targeting is potentially reversible since there is no genomic edit. Some Cas13 enzymes...KW, Yoon S, Kim J-S (2016) Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells...

  37. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...Bacterial Plasmids. Microbiology and Molecular Biology Reviews 62(2): 434–464. Pubmed PMID: 9618448 Diaz R, Rech...RP (1987) Plasmid incompatibility. Microbiology Reviews. 1987;51(4):381–395. Pubmed PMID: 3325793 Schumacher...

  38. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...the questions.  After the code is written, it is reviewed by at least one other developer. This is another...point of collaboration for us. I enjoy doing code reviews because it’s like a little peek into the mind of...

  39. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...company you currently use. You can also find many previously validated gRNAs at Addgene. In vitro screening... and stuff ups). This is a huge improvement on previous mouse work, which could take 8 months or more....

  40. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...subcultured at densities that are low enough to prevent confluency but high enough to promote cell growth...Incidence, sources, effects, detection, elimination, prevention.” Cytotechnology 39.2 (2002). 75-90. Pubmed PMID...

  41. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...seizures or some other unwanted phenomenon or it could reveal something fascinating. Behavior is based primarily...behavioral test might be contextual fear conditioning (reviewed in [4]). Contextual fear conditioning is a behavioral...

  42. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second crossover... in 5 days. Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab...

  44. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...encoded tag and exogenously added components. For a review see Lotze et al. (2016). To date, small probes ... tools engineered from phytochromes." Chemical reviews 117.9 (2017): 6423-6446. PubMed PMID: 28401765....

  45. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...CRISPR editing CRISPR, of course, has started a revolution in gene editing. However, while CRISPR is remarkably...CRISPR-based approaches is described in several recent reviews (Howes and Schofield, 2015; Moser and Hirsch, 2016...

  46. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...antibody dilution — both factors we’ve covered in our previous blog posts (1, 2) on western blotting. If the ...confidence in the accuracy of western blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/...

  47. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...strategies. As an example, we’ll use one of my previous experiments where I harvested a melanoma tumor...single cell + live” population, acquired from the previous gating pipeline, about 53% of my cells are dendritic...

  48. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...operator is in a different room.   Design: The previous cycle made me realize we needed to focus on ways...wouldn’t be in there with them. I knew from my previous cycles that I wanted to be able to talk to people...

  50. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...expensive sequencing fuels a sequencing revolution that still prevails. Sequencing whole genomes or profiling...
Showing: 301 - 350 of 604 results