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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 414 results for: vent

Showing: 121 - 140 of 414 results

  1. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...context, it’s hypothesized that base removal helps prevent the invading genome from “recovering” its original...cannot switch guides. While this fidelity would prevent other small nuclear DNAs from binding NgAgo, it...

  2. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...into the chromosome. Thus the term “episome” was eventually dropped and we’ve been using "plasmid" ever since...few years genes from different bacterial (and eventually mammalian) species were cloned into plasmids ...

  3. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...interested in XP out of a desire to understand the early events in the transformation of healthy cells into melanoma... they now think that mutations in the gene may prevent neurons from returning to basal conditions normally...

  4. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...that we aren’t still pitching for feedback.  Your venture should continuously evolve and respond to feedback...number of experts, including Harvard/MIT faculty, venture capital and angel investors, and business development...

  5. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...connection between molecular- and cellular-level events and behavior. It’s a problem that he considers ...change has a particular advantage for observing events taking place inside cells that are on the move,...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ..., imaging of high frequency glutamate signaling events, and accurately differentiating between synaptic... enable scientists to robustly detect signaling events in the brain and beyond. These new iGluSnFR3 variants...

  7. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...professional societies hosting virtual networking events. Download Addgene’s Science Career guide to learn...families at home, the STEM career exploration site Inventing Heron has a radio reporter challenge, and code.org...

  8. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...educational and great for lab life. These apps prevent you from constantly searching the interwebs for...information in the app is restricted to the NEB inventory only, the restriction enzyme sequence resource...

  9. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have a colleague who seems fun ...strawberries. Grand gestures are memorable! Attend events for one organization or go to the same conferences...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... spatio-temporal precision with which signaling events occur in a physiological setting. Genetic techniques...Recombination (HR) or Single Strand Annealing (SSA) in the event of a DNA break. Next, the gRNA being tested is expressed...proteins, determined by competing recombination events at the genetic level. Upon Cre/loxP recombination...

  11. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...approach, your workflow, or your physical setup - to prevent the mistake, just like you would change variables...reagents from left to right as they use them.    Prevent Interruptions If you need to focus, a large set...

  12. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...F0 (the measure of signal change during calcium events), Greater brightness, Less signal distortion from...above. The number of action potential (AP) spiking events are indicated below the trace; asterisk indicates...

  13. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...interneurons (83895). See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype...

  14. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...target region into the N-terminus of mCherry. To prevent alternative start site initiation, the lab removed... this one mutated methionine was not enough to prevent alternative initiation, so they mutated the methionines...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ... Cpf1 can bind and cleave the lentiviral RNA, preventing packaging of the virus.  Reversing the orientation...Cpf1-mediated cleavage. This reversing would also prevent processing of the crRNA array when expressed in...

  17. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...contain either one or no copies of the target DNA. Conventional thermal cycling is then performed to amplify...you will use aren’t that much different from conventional duplexed primer probe assays often used in real...

  18. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...continues to make improvements to our website and inventory management systems to make way in our systems ...Software and IT Teams made major upgrades to our inventory management systems to enable efficient, tracked...

  19. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...Careful documentation will keep you on task and will prevent you from getting lost in the wide word of informatics...analyses. The same is true for scripts. Naming conventions Give every experiment a number: Numbers are ...
Showing: 121 - 140 of 414 results