...LC) is known to play a major role in the stress response circuit by integrating neuronal signalling and...activated, norepinephrine release causes a freezing response, a form of immobility caused by stress and fear...surface. The latter reflects a state of behavioral despair, and the more time the animal spends in the passive...subset of excitatory neurons (Drd1) in mPFC is responsible for the antidepressant effect. To specifically...

..... Ownership: Misunderstandings about who is responsible for what tasks or projects is a common source...customer support element amongst their other responsibilities. Knowing which aspects of customer support... require a manager to explicitly assign the responsibility in question to a specific person or team. ...one of your strengths. Empathizing with people, especially people with whom you strongly disagree, is a ...

...species on a single sample, you can then use the corresponding secondary antibodies conjugated to distinct ...), and will share some of their results and corresponding protocols. Other researchers may also have tested...Reviewing available antibodies can seem overwhelming, especially when there are dozens of products that all claim... find an antibody in which you are confident, especially if you work in an organism that is underrepresented...

...synthesized enzyme. Biotin protein ligase (BPL) is responsible for this highly specific post-translational protein...biotinylated. In E.coli for example, the BPL, BirA, is responsible for biotinylating only one lysine in the entire...Esmond E. Snell was able to purify the protein responsible for binding biotin, avidin. Avidin was aptly ...your protein of interest or primary antibody respectively. Avidin bound to a reporter is then added to...

...wheat, mushroom, and rice (Khatodia et al., 2016). Despite the almost universal functionality of the CRISPR...used to drive expression of small RNAs in their respective species. Addgene carries >30 “empty gRNA” backbones...activate or repress target genes in plant cells, respectively. Cas9 expression is commonly driven by plant-derived...excellent alternative genome editing technologies especially in human cells. In contrast to homology directed...

...differences in physiology, intelligence, and human correspondence that give them an edge as a disease mode (Iannaccone...also easier to study and at times more closely corresponds with human diseases and conditions. For example...scientific history, highly characterized genome, and widespread utilization, Arabidopsis has made significant...Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research development. Arabidopsis...

...very rapidly in response to a single light pulse whereas others have slower responses (Gunaydin et al....different opsin (natural and engineered proteins responsive to light in combination with retinal) variations... along a pathway, there can be very different responses to long vs. short stimulation times or frequencies...

... function. When a select group of people are responsible for a group of specific processes, they must ...tasks done. At Addgene, the Scientist Team is responsible for guiding new deposits into the repository,... not someone (or someones) taking on these responsibilities on a team to which you belong, consider stepping...roles they’re meant to fill and what their responsibilities are. That’s a subtle but important distinction...

...sequence, a part of the env gene containing the rev response element (RRE), an internal promoter, and the desired...generation of replication competent lentiviruses (RCL) especially if you are working on HIV positive human cell...generating RCL is low but extra care has to be taken especially if you are working with proto-oncogenes or with...the Tet-on system. In the Tet-off system a Tet-response element (TRE) is placed upstream of the promoter...

...to their Biosafety Level-2 or 2+. Please Note: Despite the fact that AAVs themselves are not pathogenic...expression (up to 9 months in mice [2]). Low Immune Response: Compared to other viral vectors (e.g. Lentivirus...Adenovirus) AAVs cause the least amount of immune response. Any investigator who is using an in vivo animal...model should consider the possible elicited immune response from viral infection. Animal safety is a top priority...