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We narrowed to 809 results for: nin

Showing: 351 - 400 of 809 results

  1. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...Addgene The links below pull up lists of plasmids containing the indicated gene, and you can subscribe to ...pages to learn when Addgene receives new plasmids containing these genes. As with the PIs above, many of these...your work. The links below direct you to pages containing lists of plasmids from the indicated publications...following articles are just a small cross-section spanning 20 years of research. DNA Cross-Bridging Shapes...

  2. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use...plasmids expressing SARS-CoV-2 proteins at the beginning of April. Now, we've turned some of the most requested... genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control...Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity, particularly...

  3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...are available for request? Currently, plasmids containing all SARS-CoV-2 protein coding genes in E. coli...Addgene. A set of mammalian expression vectors containing various spike protein variants are also available...received Ginkgo’s first deposited set of plasmids containing SARS-CoV-2 protein coding genes in E. coli and...By leveraging our core strengths of intensive screening and the use of high throughput platforms, Addgene...

  4. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...drastically affects their stable folding and efficient functioning. But what about placing the mesophilic hosts ...consider them worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse...coli! Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a...conventional academic settings, these are an eye-opening experience for cultivating a nuanced understanding...

  5. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...interest. Isotype controls are used to make sure the staining you see is due to target-specific antibody binding...binding rather than nonspecific background staining. Isotype controls are used in antibody-based applications...switch to expressing a different isotype, the intervening DNA sequence is deleted, which places the variable...domain gene cluster. DNA recombination can delete intervening heavy-chain constant domain genes, placing the...

  6. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this step. Then...antibodies will exhibit off-target binding to unintended proteins. This is because monoclonal antibodies...then used to produce all lots of the antibody, meaning that the antibodies are largely identical between...antibody that is actually produced by the hybridoma, meaning that there will be changes between lots over time...

  7. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ... translation (Noren et al., 1989). In the late nineties, this was developed into an E. coli system, with...will be needed to introduce the gene of interest containing the chosen codon that will result in the incorporation... parameters the same for all three components, meaning that the final protocol may involve a sensitive... located at Oregon State University focused on turning GCE tools developed in the literature into robust...

  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...introduce frameshift mutations in genes of interest, turning expression off permanently. Viral vectors can ... preserving cell viability. Library delivery Screening libraries, large collections of biological independent...library cargo delivered are controllable. For many screening applications, it is essential to deliver a single...Gabuzda D, Crowe SM, Mills J, Lewin SR, Brew BJ, Cunningham AL, Churchill MJ. Pathogenicity and immunogenicity...

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...time-lapse images, quantitatively evaluating antibody staining, or counting cells are now routine, open-source...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...via globally established makerspace networks and training users via workshops, seminars, or hackathons greatly...research. The EU, for example, is calling for the opening of sources within the next few years, with the ...

  10. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...involves two basic components: the pigments (anthocyanins), and the plant circadian clock. Petunias have...the biochemical pathways involved in central anthocyanin metabolism are well understood. Additional work...comprehensive understanding of the genetic pieces underpinning these mechanisms – our project wouldn’t be possible...biology. There are already two comprehensive plant cloning kits available in the repository, MoClo and GreenGate...

  11. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...which Susan replied, “What’s a science writer?” Learning about SciComm by doing SciComm Writing had always... terms of PR types of things or communications planning.” To beef up her resume, she decided to complete...mind process things. Sometimes finding that uninterrupted time to be creative is challenging,” says Susan...Susan  finds important for her work include: “Listening. Evaluating evidence. Critical thinking. Being...

  12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ..., David Liu’s lab developed split cytosine and adenine base editors that can be reconstituted in vivo ...overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive...via FRET. Image from Beck and Gong, 2019. Broadening the spectral diversity of GEVIs can enable resolving...Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists to use...

  13. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...other antibodies in the experiment. If you are planning to use multiple antibodies in a single experiment...independent antibody strategies that compare the staining patterns of multiple antibodies against the same...express a tagged version of the antigen and compare staining of the antibody to that of the anti-tag antibody...negative controls as you get your assays up and running.  Ed.'s note: More questions on antibody validation...

  14. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...permanently or temporarily, we’ve got you covered. Defining your control requirements To pick the best tool...permanent control mechanism? Where is the control happening? Is the regulation of an exogenously introduced... Turn it on forever Permanently increasing or turning on expression of a new protein is a cinch. Stably...et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing...

  15. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...withdrawn from the US market because of life-threatening health effects. Eight of these posed greater ... important to get the research right from the beginning. This is the goal of Gendered Innovations. This...biological variable in all NIH-funded research. Janine Clayton, Director of the Office of Research on ...compared to that of human clinical trials. And, as Janine Clayton points out, late-stage clinical studies...

  16. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...photoconvertible variants, it seems like GFP is always learning new things. Now, work from Connie Cepko’s lab ...as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization, Tang...channelrhodopsin-2, commonly used in optogenetics, opening up the possibility of adapting GFP lines for optogenetics...Listen to Our Podcast Interview with Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed...

  17. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later,...When using the ClonTracer system, lentiviruses containing these barcodes infect the target cell population...infected a non-small cell lung cancer cell line containing an activating mutation in epidermal growth factor... Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource...

  18. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...transition out of the academic sphere into a different meaningful career in science. Supervisors in the academic...scientific backgrounds. For a stated purpose of transitioning a scientist from academia to industry, you might...although most scientist coaches can help with transitioning out of academia (because so many scientists ...Website Check out Our Career Page for Recent Job Openings Find Plasmids to Expedite Your Research   ...

  19. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...single sentence and figure must be clear, concise, meaningful, and supportive of the mission of the application...of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence. Below are...alternative solutions to demonstrate thorough thought planning on the matter. The more detail (within the page...Application" by Vid Mohan-Ram in Science Careers "Beginnings - How to write your first grant proposal" by ...

  20. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ... allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation of...important to test different lengths and sequences, as unintended secondary structures in your pegRNA could inhibit...3′ end to protect them from degradation. When designing epegRNAs with large structured motifs like mpknot...

  21. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... calcium binding proteins (e.g., calmodulin or troponin C). Their fluorescent readouts are used to monitor...can be delivered to intact tissues and organs noninvasively and target a defined population of cells (e....to endogenous calmodulin. Multiplexing Are you combining your calcium reading with other measurements, ... is a FRET-based calcium reporter derived from troponin C. It is engineered with a reduced number of calcium...

  22. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...Gate Assembly for cloning, you might be familiar with the rules of thumb for designing your overhang sets...Additionally, this assembly, which used only antibiotic screening for selection, failed under normal cycling protocols...complexity allowable by a favored, fairly easy cloning technique (which may be of particular interest ...

  23. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... genome, the virus is replication incompetent, meaning it can’t replicate and continue the infection cycle...known to cause respiratory diseases in humans, earning this virus a BSL-2 classification. Lentivirus (... can be used for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge...trained in each new place (and pay attention to the trainings!), even if you know guidelines for best practices...

  24. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded... is sufficient to disrupt dimerization while maintaining the bright and stable fluorescence of the original...also named their variant mStayGold. It is worth mentioning that Kiryl Piatkevich and colleagues generated... Figure 3: Overview of library generation and screening that led to identification and characterization...

  25. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...or failure and demands attention, practice, and honing of your skills from the start. Just like with any...are unlikely to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor...may make your perfectly reasonable paragraph unintelligible to a lay audience. My lay paragraph typically...a Grant Writing Professional Facilitate Career Planning and Personal Development with an IDP Get Tips ...

  26. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...project was spent selecting guides, designing primers, and running analyses. New team members have taken...guiding principles that we've followed since the beginning. It's free and open to all. The project is funded...blog posts Learn about some considerations when designing gRNAs Find other CRISPR software Resources on...

  27. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...Cas9s from other species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in ...SpCas9-VPR in activating transcription. Sites containing NG, GAA, or GAT PAMs averaged 56-91% transcriptional...improved, with a smaller increase in NGA editing. Adenine base editor xCas9 3.7-ABE also follows the same... at an NGG PAM (69 vs 48%) compared to SpCas9-containing ABE7.10, as well as improvements at NG and GAT...

  28. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...broke the PE efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024). Their optimized...and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and a C-terminal SNAPf...for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings...postmortem brain samples. Results show positive staining in tissues from patients who had confirmed neurodegenerative...

  29. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    .... P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (2015). An updated... scale that other Cas effectors can’t, while maintaining relatively high efficiency. These alterations... boundaries defined by anti-CRISPR proteins or running into other proteins naturally bound to the DNA....

  30. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...after initial mouse training. Other techniques require more skill and additional training, going from intermediate...All researchers must go through the proper basic training prior to conducting mouse experiments. Some of...preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge...

  31. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... cells, such as those within hair follicles and lining the digestive system, causing discomfort and illness... enabled researchers to begin discovering and designing molecules that are showing some promise in their...collection is a set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that... Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used...

  32. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But what about...incomplete. Protein expression strains are not good for cloning Bacterial strains adapted for protein purification... genes and can therefore be cultured in media containing half the normally recommended antibiotic concentration...plasmid/strain combination individually, based on determining the cell density or by harvesting and miniprepping...

  33. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...Glycine and Proline residues found on the C-terminus meaning the upstream cistron will have a few additional... clone your gene of interest into the multiple cloning site up or down stream of the IRES or 2A element...you can start with a plasmid that has multiple cloning sites flanking the multicistronic element(s), or...Addgene's Plasmid Guide for more molecular biology and cloning information. ...

  34. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium...insertions (usually via seed growth on antibiotic-containing media) A similar process occurs in nature; ...plants should show no signs of stress, such as anthocyanin production, or disease, such as fungal growth... in a transformation in another 24 hours. The remaining starter culture can be used for colony PCR and...

  35. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...to COVID-19. In our lab, we were interested in examining the effect of COVID-19 on worldwide scientific...cases being reported (right, red), likely due to quarantining {% video_player "embed_player" overrideable...candidate at MIT. His research uses applied machine learning and statistical methods for fundamental scientific...interested in using selection, evolution, and machine learning to solve difficult problems in chemistry and chemical...

  36. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... misconception. I find personal bias often unintentionally plays a big role in what people think of as...as "the best way" to communicate their science, meaning that they will heavily evangelize for the mode ...cost or freeware alternatives and how steep of a learning curve it will be to get to the necessary level...Static. Often requires both artistic and scientific training. Cartoons may be read as “for children” or may...

  37. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...search results: More than 200 different GCaMP containing plasmids are available for distribution. If ...already created and deposited it with Addgene. Combining search terms with filters lets you drill down ...) Vector Type: What type of experiment are you planning? (e.g. Retroviral) Species: Specify the source...bacterial resistance marker used to grow plasmid containing bacterial cultures Bacterial Resistance: Use ...

  38. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...type of interest. Repeat rounds of infection and screening by PCR enrich for capsid variants with increased...single-cell anatomical study and neuronal tracing, aka determining where a neuron’s axons and dendrites extend to...brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB’s or AAV.PHP.S’s great neuronal transduction...Yoo BB, et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

  39. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...neurotropic variant of AAV9, is better than AAV9 at gaining access to the CNS of C57BL/6J mice following IV...6J mouse strains likely contributed to the unintentionally selection of a virus with mouse-strain dependent... to select novel AAV capsid variants could unintentionally limit the utility of candidate capsids.  Are...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

  40. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... feeling of camaraderie one gets from a well-functioning lab team. Obviously not everyone gets this feeling...environments outside of academia provide a formal training regime and the right set of tools to help you ...non-scientists! Maybe you’ve just spent 5-10 years learning to “speak science,” and virtually every person... years of focusing on my technical skills with cloning and lentivirus production, I didn’t really start...

  41. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic of the evolution...- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over conventional antibodies... secreted into the supernatant. Four plasmids containing different nanobodies (NB) were generated in pMAK... dual-deaminase base editor enables concurrent adenine and cytosine editing. Find the plasmids at Addgene...

  42. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016) C2c2 is a single-component programmable...Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017... L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional Characterization...Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F (2015) Cpf1 Is a Single RNA-Guided ...

  43. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...truth is, there is a lot of software development happening at Addgene under the hood. A significant portion...moment a deposit kit is sent to a depositor, to streamlining the order fulfillment process to reduce viral...language and it took us a total of two years, from planning to completion, to do this upgrade while continuing...principles for open science. I hope you enjoyed learning about Addgene’s software development team and ...

  44. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...are typically lysed in a non-denaturing buffer containing non-ionic detergents, such as NP-40 or triton-X...nonspecific proteins from the sample prior to beginning the IP.  During the IP, the target protein in ...Immunoblotting.) For mass spectrometry, elute in a urea-containing lysis buffer. Alternatively, the protein can ...control, confirming that the capture antibody is functioning as expected. In order to identify any non-specific...

  45. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...managing a lot of references. I cite as I write, meaning I use the software to add the citations in real...write. Things are going to get a little crazy (meaning you are probably going to cite hundreds of references...better to keep your references organized from the beginning. I also recommend using the citation style of ...relevant to you, note it, and move on. At the beginning I felt like this was cheating. Eventually I learned...

  46. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...antibacterial drugs. Resistance can be intrinsic, meaning a basic characteristic that naturally occurs in...especially if the infection is serious or life-threatening, treatment will often begin before this information...results. While this is not the only method of determining drug resistance status, it is a very common, ...essential component of preventing infection, as is cleaning and disinfecting frequently touched surfaces like...

  47. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...immortalized human cell line. He proved this by examining whether the cell lines had isoenzymes and genetic... piece of paper on the front of the incubator containing the passage number, cell line, and date, which...He studied biochemistry at Warwick University, gaining extensive cell culture research experience through...Cell Culture Guide Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen Cell Culture Basics...

  48. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...of control plasmids Part of planning your experiment includes determining what factors need to be controlled...independent variable you are testing and not some other unintended factor. Control plasmids are used to minimize...systems have many variables. However daunting designing and executing these steps may be, proper controls...

  49. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...of E. coli for DNA propagation. While great for cloning purposes, these E. coli strains are not usually...Use BL21 (DE3)   Basic IPTG-inducible strain containing T7 RNAP (DE3) F- ompT lon hsdSB (rB- mB-) gal... (DE3)** Rifampicin RecA-deficient; allows for cloning and expression in same strain F- recA1 hsdR(rK12...most experiments, the lac promoter can “leak,” meaning that a low level of expression exists even without...

  50. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... and Olsson, 1971), plant lectins (wheat germ agglutinin WGA, Schwab et al., 1978) or fluorescent molecules... mapping of the neuronal circuitry involved in learning and memory (Kohara et al., 2014; plasmids at Addgene...Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... motor to auditory pathway important for vocal learning. Nat Neurosci 20(7), 978–986. PubMed PMID: 28504672...Javoy-Agid F., and Agid Y. (1978). Labeled wheat germ agglutinin (WGA) as a new, highly sensitive retrograde tracer...
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