We narrowed to 604 results for: REV
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TypeBlog PostPublishedMarch 7, 2019, 2:39 p.m....Agency, which started making regulations such as preventing manufacturers from dumping hazardous waste directly...
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Management for Scientists: Seeking Feedback
TypeBlog PostPublishedJan. 8, 2015, 2:34 p.m....inefficient. An effective team regularly seeks expertise wherever it can be found. Listen to our podcast interview... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....vivo gene knockout in immune systems. CHIME We previously developed CHIME (CHimeric IMmune Editing system... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m....about company culture at Addgene See some of our previous Halloween costume contests ... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog PostPublishedMay 17, 2016, 2:30 p.m....these plasmids have been used successfully in peer reviewed articles. If you’re targeting your favorite gene... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog PostPublishedJan. 2, 2014, 3:44 p.m....control who can edit the documents and track the revision history. Now you’ll be able to make updates to... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog PostPublishedNov. 27, 2019, 2:03 p.m.... stimulation in a different brain area can then reveal the cells bodies of interest. The toolbox for ... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....number when comparing two plasmids, we recommend reviewing the cited papers to determine whether the plasmid... -
A Better Way to Get Customer Support: The Help Center
TypeBlog PostPublishedDec. 10, 2015, 3:30 p.m....allows you to access our Help Center’s content from wherever you are on our site. Simply click on the icon,... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....is not in the first pile, it may never even be reviewed regardless of personal connections to the hiring... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....sequence to enable them to recognize and fight it forever. He emphasized the potential of this extraordinary... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m....looking in the direction of biosensors capable of revealing, for example, how muscles work inside a living... -
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....Syntaxin3, and osa-miR528. My manager, Katherine N., reviewed the list and followed up with her manager. They... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m.... key to unlocking new perspectives or even revolutionizing your research. Why serendipity matters Serendipity... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog PostPublishedMay 2, 2023, 1 p.m....identification methods missing something? Here we will review some time-tested gene identification systems that... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog PostPublishedMarch 15, 2018, 12:59 p.m.... scientists Mollie Schubert and Shuqi Yan for reviewing this blog post. Additional Resources on the Addgene... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog PostPublishedJune 4, 2020, 1:22 p.m....EDTA chelates any divalent cations like Mg2+, preventing the activity of DNase. While this is very important... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog PostPublishedJan. 21, 2020, 2:30 p.m.... submit their published data for rapid curation review and upload. In addition to our main website, all... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m....response. The function of Kolmer-Agduhr cells had previously been a mystery, but through the control offered... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog PostPublishedJune 11, 2019, 1:16 p.m....figures in PPT 🔬 Also, the base version is free, forever 💜 https://t.co/KJpyxgxeQt pic.twitter.com/Ho9SQlyUOP... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m.... Blog Resources on Addgene.org Check out Our Revamped Protocol Pages to Jumpstart Your Next Experiment... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog PostPublishedNov. 1, 2016, 2:30 p.m.... the 3’ end of the multi-cloning site, and the reverse primer is located in the 5’ end of the multi-cloning... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....with mouse cortical neurons, far out-performing previous attempts to engineer potassium-selective channelrhodopsins... -
Management for Scientists: Managing vs. Leading
TypeBlog PostPublishedMarch 31, 2015, 4:08 p.m.... Goldsmith, with Mark Reiter Harvard Business Review 10 Must Reads: On Leadership (Articles on Leadership... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m....helpful advice from the successful PIs who were previously featured in "9 Tips to Achieve Success in Academia... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....directly kill bacteria (bactericidal) or slow growth/prevent cell division (bacteriostatic); however, the distinction... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....read more about what makes a good mentor, see the previous post in this series. Check out Joanne's Reddit... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....into proteins, or, in the case of retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....differences are subtle, chimeric antibodies should be revalidated before use. In addition, chimeric antibodies ... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....short culture periods (less than 20 passages) to prevent these issues from popping up, and if your research... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....kick off at. Whatever you’re working on, start wherever it makes sense to start and try to be as chronological... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....mean? How did the researchers solve the problem preventing them from moving forward? Answering these questions... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m...., I had the chance to carry out a comprehensive review of the literature informing the notion of “heading... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....antibiotics. Simon Moore and Paul Freemont’s lab previously developed such a system in Streptomyces, a prokaryotic... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....studies immunity and the host-pathogen interface, previously developed a high-throughput neutralization assay... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....expression of the mAb-encoding genes, die, or fail to revive after cryopreservation (Bradbury & Plückthun, 2015a... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best to propagate AAV constructs... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m.... and structural deformation, these probes are reversible and do not cause structural deformation or photo-induced... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....biggest potential to transform patients' lives. Previously Stegmeier was the Head of Oncology Target Discovery... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m....run that last additional experiment requested by Reviewer #3. But why isn’t the plasmid here yet? A quick... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....ended up taking the ‘dead-end’ path. The CellTags revealed that cells with common ancestors tended to have... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m.... different about academia? Publishing in peer reviewed journals is absolutely necessary for success One... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....for Enhanced CRISPR Genome Editing CRISPR has revolutionized how researchers make knockouts and other types... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m....rebind and nick the newly ligated DNA. Salt helps prevent topoisomerase I from rebinding, which results in... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m.... Stasevich lab tested several scFvs scaffolds previously proven to fold and function in living cells. ... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction of... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....skills you should focus on improving and will help reveal potential opportunities beyond academia. Once a... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog PostPublishedFeb. 13, 2024, 2:15 p.m....to model individually-specific factors, such as previous exposures to that specific antigen, pre-existing... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m.... headache. How do I maintain ITR integrity and prevent unwanted mutations? During propagation, mutants... -
Trading Spaces - Visiting Scientist Edition
TypeBlog PostPublishedAug. 22, 2023, 1 p.m.... don’t understand something. Offer to help out wherever you can and don’t forget to express your gratitude...