We narrowed to 293 results for: gne

1. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ....  Similar to a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in ...

2. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...purification is now a reality thanks to OptoNBs. Recently designed by the Toettcher Lab, OptoNBs are photoswitchable...

3. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...differentiation in a high-throughput manner. They designed the pXPR_053 lentiviral expression vector, which...

4. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ... shift requires deliberate preparation and a willingness to embrace new responsibilities. After my second...

5. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   Published

   Sept. 26, 2024, 1:15 p.m.

   ..., the use of LIC is often limited to specially designed plasmids. Sequence- and ligation-independent cloning...

6. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ... that you know how to read flow plots and have designed your first flow panel, you’ll load your samples...

7. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ... also show you how multiplexed arrays would be designed, if that’s your end goal. CasPEDIA also features...

8. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   Published

   April 27, 2023, 1:15 p.m.

   ...References 1. Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access...

9. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   Published

   March 21, 2023, 1:15 p.m.

   ...target cells and organisms, and when that cargo is designed to make you sick…well, you feel it. But the same...

10. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...other experiments. And, while the BLITZ system was designed and optimized with zebrafish in mind, it can easily...

11. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...Bhopal. All illustrations / figures featured were designed by our team member Kallol Mozumdar. Sreepadmanabh...

12. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...Addgene page went up last month, over 340 people have signed up to receive alerts when plasmids become available...

13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...the plasmids made by Xie et. al are specifically designed for plant expression, PTGs will likely be adaptable...

14. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...first generation of CRISPR-Cas9 plasmids were not designed with gene activation in mind. “We had not managed...

15. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ... don’t overdo it (some of my best studies were designed over late night poker games at Gordon Conferences...

16. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ... initially consist of 2-3 individuals that are aligned in vision and outcomes. The team must work well...

17. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...This technology allows you to create a pair of designer proteins that will introduce a double-strand break...

18. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    Published

    Feb. 2, 2018, 3:20 p.m.

    ...Susan. There are also team meetings and Susan is assigned to cover the Clinical Research Division at Fred...

19. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...novel RNA-protein interactions, Ramanathan et al. designed an RNA bait containing three well conserved but...

20. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...ortholog requires a different PAM sequence, a gRNA designed for one dCas9 should be specific to that ortholog...