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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 414 results for: vent

Showing: 161 - 180 of 414 results

  1. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...many many times in a short time period. PCR was invented in the early 1980s by Kary B. Mullis who later...to design PCR primers.  Types of PCR Since the invention of PCR, different PCR methods have been developed...

  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...at the 5’ cap so that only a single translation event occurs for each mRNA. However, some bicistronic ...ITAMs ensures effective negative selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are also...cautious during the aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced...

  4. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...your boss needs someone to step up, when a new venture presents itself even outside of work, consider ... for working with lots of students, managing inventories in the lab, or organizing a research project,...

  5. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...and Pol, increasing the number of recombination events necessary to create a replication-competent virus... vectors contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer...

  6. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...site due to its proximity to the cleavage site, preventing future edits. In contrast, since Cpf1 cleaves... has not yet been experimentally confirmed. To prevent new editing post-HDR, repair templates should remove...

  7. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...Once a sample enters Addgene’s lab, a series of events is triggered. Each step is tracked through barcoded...people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours of valuable time...

  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... structure, shielded from quenching by aqueous solvent. This tightly-packed structure explains the importance...mutations are acceptable. GFP's main advantage over conventional fluorescent dyes of the time was the fact that...

  9. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...targets with high specificity and affinity, were eventually called aptamers, from the Latin aptus, meaning...presence of that metabolite (Fig.2). Spinach eventually evolved into Spinach2, a more stable and brighter...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...In this blog post, we will cover three ways to circumvent this limitation: 1) the use of novel S. pyogenes...-type SpCas9. The number of off-target cleavage events for the VQR and VRER variants is similar to wild-type...

  11. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...accompanying sequencing data collection and analysis eventually outpaced our software, even with continuous updates...containing our new process) on our local computer. Eventually, our workflow would handle plasmid sequences ...

  12. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...we give people the opportunity to speak out and prevent groupthink? Here are some ideas that I have found...our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ...

  13. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...species and isotype of their primary antibody. To circumvent this, a recombinant protein, Protein A/G, was...phosphatases that may degrade the target protein. To prevent this, include protease and phosphatase inhibitors...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... the beginning I felt like this was cheating. Eventually I learned that it’s practical because there simply...how elusive a free two hour block of time was. Eventually, I learned that I was most productive in short...

  17. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...productivity, and many have mechanisms in place to prevent the burden from getting overwhelming. These mechanisms... and equity research firms, biotech start-ups, venture capital firms, and more. Essentially any role in...

  18. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... in a separate backup liquid nitrogen store to prevent loss from equipment malfunctions. One Master vial...approach, the many mistakes of the past can be prevented and we can progress faster to desired clinical...

  19. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...stimulating an ill-defined region of the brain. With the advent of optogenetics, ICSS can now be done with neuronal...Differential control of learning and anxiety along the dorsoventral axis of the dentate gyrus." Neuron 77.5 (2013...
Showing: 161 - 180 of 414 results