... sample and subject to isothermal recombinase polymerase amplification (RPA) using a commercially available...reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ reporters that...transcription. This is then amplified by PCR using Taq Polymerase and then transcribed to provide RNA substrates...instead of RPA. Unlike RPA, this technique uses Taq polymerase which is widely available and less expensive....

...developed to quantify RNA transcription by RNA Polymerase III. By expressing Corn under promoters for transcripts... transcripts uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence...effect of mTOR inhibition on the activity of RNA Polymerase III. Red and orange fluorescent aptamers Corn...PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription using a photostable RNA–fluorophore...

...biology and biotechnology purposes.   Once a polypeptide is biotinylated (more on this below) it can be...Northern Blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using a biotinylated ...commonly used this method to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003...

...single stranded regions with homology to anneal a polymerase, to fill in the gaps a DNA ligase, to seal the...than 200 nucleotides before the annealing and polymerization steps could occur. Secondly, it does not work...instead of using the exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or ...methods that have been described--CPEC (circular polymerase extension cloning) and SLiCE (Seamless Ligation...

...enter the cell with the concentration gradient to depolarize it (Nagel et al., 2003). Inhibitory opsins are...Na+) against the concentration gradient and hyperpolarize the cell (Mattis et al., 2012). Bicistronic ...considering for your experiment, note that you cannot extrapolate results from one cell type to another, as they... A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES is an optogenetic tool developed for bidirectional...

...photoactivatable, optogenetic accuators to depolarize or hyperpolarize membranes. Traditional FRET reporters... sensor, membrane depolarization increases the BRET signal whereas hyperpolarization decreases the signal...

...join any fragments end-to-end without the exo/polymerase generation of annealed complementary regions....and even include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase...Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping enzyme superfamily of...for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum....

...Updated March 6, 2020. After months of input and polling, Blugene's new virus friend finally has a name!...participation in suggesting names and voting in our poll. Now, we'd like you to meet..... *drumroll* Aavery... out this form here linked below. We’ll keep the poll open until February 28 5PM EST and we’re excited...

...guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine...binding to the template strand (strand used by RNA polymerase II for transcription) are more effective for ...editing! Pooran Dewari is a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine...immunity and mammalian genome editing via RNA polymerase-mediated dislodging of Cas9 from double strand...

...produce molecular biological tools like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins...construction of new plasmids. Dr. Duedu will also use polymerases produced using plasmids from Addgene to test ...

... uses do you foresee for this tool? Howarth: Polyproteins are useful for mechanical study of proteins ...proteins by atomic force microscopy (AFM). Maybe polyantibodies could help in the capture of other rare cell ...References: SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture...

...microelectronic fibers made from thermoplastic polymers that can work with all the bells and whistles....called thermal drawing, essentially where blocks of polymers are heated up and pulled to a desired thickness... small molecules.   Fun fact: A 1 cm block of polymer can generate many meters of fibers!     ...

...biochemistry, microbiology, epidemiology, and bioanthropology. Studying human health science made me happy...greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived...

... the reverse transcriptase, protease, and Bst polymerase constructs from their assay to Addgene.  Drew...expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step RT-qPCR reaction...Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020...

.... The topic of unionization continues to be a polarizing one. High-profile universities remain outspoken... panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington Office Ben Corb - Director... in the intersection between science and public policy. Follow David on Twitter @driglar.   References...

... opposite directions away from the ORI. A DNA polymerase completes the formation of each initiation complex...after a short section of it is compelted by DNA polymerase. The E. coli origin contains 11 methylation sites...these replication forks and is followed by DNA polymerase which synthesizes the new daughter strands. Similar...

...advantage of the program. Start the program by polling the audience with a few questions. Who is out there...in response. I am always ready with some written poll questions to get things started. Give a couple of...want to take over? It is the moderator's job to politely but firmly get them to stop talking and redirect...

...Addgene blog   Droplet Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview and Applications...Applications Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer...SYBR Green I and TaqMan quantitative real-time polymerase chain reaction methods for the determination ...

...fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out a pooled set of...topoisomerase I cut site, toposisomerase I, and a taq polymerase-generated insert. When mixed, topoisomerase I... could also be a great choice, because the T4 polymerase needed for this method is relatively inexpensive...

... can then be translated at the ribosome into polypeptide chains that make up the primary structure of ...purified for use in molecular biology research (eg. polymerases and other enzymes might be purified and used ...translation machinery. These include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free...