...set up a standard PCR reaction (primers, dNTPs, polymerase) using the supernatant of lysed bacteria as template...standard PCR reaction: combine template, primers, polymerase, and dNTPs and then incubate with a standard ...heating step of the PCR reaction. A standard Taq polymerase is sufficient. Controls: Controls can make ...

...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is direct in this species...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke...

...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...centrifugation and then by filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then ...lentiviral preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated...

...which contains a promoter, the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses... inactivated by the complement system in serum (DePolo et al., 2000). Other envelope proteins for pseudotyping...–398 . https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL...

...Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio...Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective delivery of large...

...with a “sloppy” version of PCR that results in a polymerase error rate of up to 2% per nucleotide position...modifications: 1) increased concentration of Taq polymerase, 2) increased PCR extension time, 3) increased...degenerate primers and the high-fidelity Phusion polymerase are used to introduce point mutations into the...

...of useful, entertaining science lab, career, and policy content. Watch Joanne give her "Not" Networking...almost anyone with enough practice. I always take a poll of scientists in training to see how many in my ...other ways to engage the audience. Take an informal poll by asking for a show of hands on a particular question...

...from Worcester Polytechnic Institute where he investigated how plants achieve polarized cell growth in... in Biology and Biotechnology from Worcester Polytechnic Institute. What is RNAi? The loss-of-function...

...cation channel, activation of which results in depolarization and subsequent firing of an action potential...results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential generation...spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through hydrogen...

...walking tour of Buddy the Elf’s journey from the North Pole to New York City. Check out their video below. ...interpretation of Buddy the Elf's journey from the North Pole to New York. #ELF #DeckTheLab #ProudPIhttps://t....

...amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters...message delivered by many of the speakers.  As Dr. Polka said as she started things off, “We are all capable...

...Krzysztof Chylinski (who coincidentally speak the same Polish sub-dialect making their Skype communications extra...evolution A Powerful New Way to Edit DNA (by Andrew Pollack of New York Times)   ...

...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...

...previously yielded valuable enzymes. Famously, Taq polymerase – from Thermus aquaticus bacterium – was discovered...make them extremely dangerous, we used telescoping poles to collect water samples from a distance. At each...

...Bacteriostatic 50-100 ug/mL in EtOH Polymyxin B polypeptide Alters outer membrane permeability Bactericidal...

... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted... into three distinct types and subtypes using polythetic classification based on evolutionary similarity...

...one epitope. (It can sometimes be an issue with polyclonal antibodies, so it's good to keep epitope availability...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

... low processivity (ie. the probability of the polymerase continuing to copy the template rather than dissociating...improvements to existing technologies based on polymerase processivity. Please find the MarathonRT plasmid...

...vectors (LTRs and ITRs), plasmids with multiple polyA sites, and other plasmids that contain repeating...J, Plank C, Aneja MK, Rudolph C (2019) Segmented poly(A) tails significantly reduce recombination of plasmid...

...for antibody production have several drawbacks. Polyclonal antibodies produced in animals may be variable...potential for further engineering as compared to polyclonal or monoclonal antibodies. You can learn more ...