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Showing: 121 - 140 of 262 results

  1. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid...into an animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing ...

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...allows for the following to occur: A) Multiple polyclonal secondary IgGs can bind to different epitopes... Reagent Pros Cons Antibodies Bivalent Polyclonal Can be labeled with multiple dyes Commercially...

  3. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...relevant parties, and the company at large, by polling and informal communications we made a decision ...scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (multiple choice/binary...

  4. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... challenges of science becoming increasingly politicized. It would be nearly impossible for a career scientist...science in a “fake news and post-truth era,” the politicization of science (from climate change deniers to ...

  6. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...applications, including detection of environmental pollutants, drug discovery, and disease diagnosis. As biosensors...electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2: 41. PubMed PMID: 25019067...

  7. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...acceptor) by means of intermolecular long-range dipole-dipole coupling." The emission of the acceptor fluorophore...

  9. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...producing lentivirus in Lenti-X 293T cells using a polyethyenimine transfection protocol. I also recommend searching...incubation time, antibiotic conditions, and amount of polybrene. (psst…didn’t get your library pre-made from Addgene...

  10. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...where they are incorporated into the growing polypeptide chain. One part of the tRNA contains the anticodon... codon for every amino acid across an entire polypeptide. Many researchers have created codon-optimization...

  11. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...sample is first amplified using RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-...as well as health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides...

  12. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...exposed to specific UV wavelengths or chemicals (Polstein and Gersbach, 2015; Zetsche et al., 2015). These...e7 . https://doi.org/10.1016/j.cels.2018.04.011 Polstein LR, Gersbach CA (2015) A light-inducible CRISPR-Cas9...

  13. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...Anuganti, M., Zhang, R., Lloyd, K., Jardine, J., Coppola, J., Meijers, R., Li, J., & Springer, T. A. (2024...2024.01.26.577394 Jo, M. H., Li, J., Jaumouillé, V., Hao, Y., Coppola, J., Yan, J., Waterman, C. M., Springer, T. A.,...

  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...guide to working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org ...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...thaliana dCas9 Activation of gene transcription Polstein et al., 2015 LACE CRY2 and CIB1 from A. thaliana...https://doi.org/10.1016/j.chembiol.2014.12.011 Polstein LR, Gersbach CA (2015) A light-inducible CRISPR-Cas9...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...of assembling these multi-protein fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher...sequential additions have been made, the final polyproteam can be eluted using maltose. This procedure allows...
Showing: 121 - 140 of 262 results