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Showing: 1 - 3 of 3 results
  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...promoter of lac and trp Regulated like the lac promoter Contains -35 region from trpB and -10 region from...crossover. Generally speaking, promoters in bacteria are less diverse and complex, having fewer parts than those...Cauliflower Mosaic Virus Constitutive  Active in dicots, less active in monocots, with some activity in animal...Constitutive  Murine U6 is also used, but may be less efficient.   Prokaryotic promoters     Promoter...expression through varied inducer concentrations. trp High levels of gene expression Promoter from E. coli...Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your...Constitutive  Tends to give consistent expression regardless of cell type or physiology. SV40 General expression...
  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    May 10, 2018, 1:15 p.m.
    ...TetO elements, allowing transcription to proceed (GFP expression is controlled in this manner in pJKR-H-tetR...Researchers have engineered pADH1 variants that are less sensitive to ethanol, including the “middle” ADH1...the fungus Neurospora crassa. The QUAS promoter is less leaky than UAS in the basal state, and co-expression...tac promoter: a functional hybrid derived from the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(...
  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...The applications for such a modular system are endless and, as scientists use GMAP, there will be a continuous...ferritin protein were tethered to the C-terminus of TRPV4 (a pressure-sensitive channel). The Guler lab optimized...Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry Mammalian Expression ...
Showing: 1 - 3 of 3 results