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Showing: 1 - 3 of 3 results
  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Updated
    Dec. 2, 2019, 1:42 p.m.
    ...Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP...trp High levels of gene expression Promoter from E. coli tryptophan operon Repressible Gets turned off...Ptac General expression Hybrid promoter of lac and trp Regulated like the lac promoter Contains -35 region...Generally speaking, promoters in bacteria are less diverse and complex, having fewer parts than those...expression mRNA Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, less...expression shRNA From the human U6 small nuclear promoter Constitutive  Murine U6 is also used, but may be less...from trpB and -10 region from lac....
  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Updated
    July 16, 2020, 4:31 p.m.
    ...“The tac promoter: a functional hybrid derived from the trp and lac promoters.”...Tet), TetR preferentially binds Tet and vacates the TetO elements, allowing transcription to proceed (GFP...Researchers have engineered pADH1 variants that are less sensitive to ethanol, including the “middle”...The QUAS promoter is less leaky than UAS in the basal state, and co-expression of the inducer QF can...
  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Updated
    Oct. 27, 2019, 6:17 p.m.
    ...BioID2, is significantly smaller, allows for more selective targeting of fusion proteins, requires less...the dual colour-emission biosensor Rosella consists of DsRed connected to a pH-sensitive variant of GFP...over 30 promoters and 140 genes, including constructs with unique tissue-specific promoters expressing GFP...As a proof of concept, Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent...As a proof of concept, Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent...The applications for such a modular system are endless and, as scientists use GMAP, there will be a continuous...actuator in which two subunits of the paramagnetic ferritin protein were tethered to the C-terminus of TRPV4...Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4...PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry...
Showing: 1 - 3 of 3 results