...expand the gene-editing applications and targeting scope of ABEs David Liu’s lab set out to evolve ABEs with...However, the increased editing speed and targeting scope of ABE8e comes with a price: ABE8e showed increased...

... one needs to capture a diverse range of CTCs to cope with the variation in cancer, not just the CTCs ...

...and implementation of new strategies designed to cope with current increases. These two rulings along ...

...nearly every participant. Robert Bramson, author “Coping with Difficult People,” identified seven types ...with each of these personality types is beyond the scope of this article, there are a few principles that...

...to admit that paper-based record keeping barely copes with the growing amounts of digital data generated...

...couldn’t fight effectively. I had to learn how to cope with a different climate and culture, and also needed...

...structural specificity to the viewer. Finally, modern microscope designs may further exploit the unique characteristics...non-converging or diverging) excitation light exits the microscope objective evenly illuminating the entire (wide...Therefore these are sometimes call Epi-fluorescence microscopes. (See left image above for an example image)....by pixel is slow; therefore, certain confocal microscopes use parallelization to enhance performance. In...and a reduction in signal intensity. 2-Photon microscopes restrict excitation (and bleaching) to a single... scanning as is done in confocal or 2-photon microscopes. Total Internal Reflection Microscopy (TIRF) ...the diffraction (resolution) limit of a light microscope. Due to the wave nature of light, an infinitely...

...PrakashLab #foldscope) had taken out a few foldscopes and held an improv workshop. These paper microscopes work...paper-based, affordable, and portable microscope called the ‘foldscope’ (Dr. Prakash, Stanford University...work as conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron...worked many hours in a dark room with a confocal microscope, yet I was once again fascinated by the world...when looking down a paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful...listening to’ microbes But seeing microbes through a microscope is not the only way to visualize them. You can...

...beyond the scope of this post. Are your fluorescent proteins compatible with your microscope optics? To...imaged in distinct fluorescent channels on the microscope. With the proliferation of fluorescent proteins...and that can be imaged with the optics on the microscope(s) you intend to use. An accurate determination...you are interested in are compatible with your microscope optics, you will want to compare the excitation...protein with the filter sets or lasers on your microscope. Ideally, you would like to have substantial ..., so that the protein is well excited by your microscope and the fluorescence emission of the protein ...protein is efficiently collected by the microscope. To compare the match between a fluorescent protein and a...

...cleared rodent brain, the Ultramicroscope was developed using a stereomicroscope to capture large field-...techniques that make tissues transparent are enabling microscopes to visualize the cellular architecture of whole...foundation for all subsequent versions of LSFM microscopes (3,4). After this initial application, Stelzer... single-plane or selective-plane illumination microscope (SPIM). In order to improve axial resolution ...microscopy vs. confocal microscopy In confocal microscopes, optical sectioning of a specimen is based on...photomultipliers used to detect light in confocal microscopes are less efficient than the modern cameras used...sides and merge the images, such as in the Ultramicroscope.  In addition, adjusting the thickness of the...

...Since its early years, the light microscope has been a powerful tool in almost all disciplines. In biology...experiments. Projects such as the OpenFlexure Microscope (Collins, et. al., 2020), the miCube, SQUID (...open-source hardware. Projects such as the OpenFlexure microscope or open-source modular optical toolbox openUC2...increases accessibility. Through this approach, the Planktoscope, a portable device for studying plankton using... left panel shows the path of light through a microscope. Lower left panel shows an image of differentiating...Robotic microscopy for everyone: the OpenFlexure microscope," Biomed. Opt. Express 11, 2447-2460 (2020) Hongquan...

...track them. We have a number of microscopes, including an automated microscope but we need to develop the ...skills at the regular microscope, and the scripting skills at the automated microscope  to keep our worms...

...microscopy This technique uses a fluorescence microscope where the light source is mounted above (epi)...specimen and the excitation light passes through the microscope objective lens on its way toward the specimen...occurs only at a chosen focal volume and thus the microscope captures only fluorescence coming from this volume...particular stimulus. Using an epifluorescence microscope and GFP fusion proteins controlled by promoters...

...and get focused on the task at hand. Define the scope of the meeting: Are you meeting to design an entire...research direction you’d like to pursue? Undefined scope can lead to long meetings spent on details that ...achieve the purpose of the meeting (see “defining the scope” above) or if you can cut it down to thirty minutes...

... search scoped to plasmids shows filters for expression and vector type, while a search scoped to viral...given category, and serve as a shortcut to a search scoped to that category. Catalog ID suggestions provide...

...guidelines to authors and see whether your paper is in scope for the journal’s coverage. These guidelines also... manuscript Dos: Make sure your paper fits the scope of the journal you are sending it to. It’s wasting...manuscript decide on whether your manuscript fits the scope and the interest of the journal. Be sure to check...

...Choosing Cas9 proteins to increase the targeting scope of adenine base editors The most commonly used S... be edited efficiently. To expand the targeting scope of ABE8, the team replaced S. pyogenes Cas9 in the... the ABE8 complexes enables a broader targeting scope for base editing.  Try ABE8 base editors for your...

...Why Expansion Microscopy?  Traditional light microscopes are limited in the level of resolution that they...with a conventional, diffraction-limited light microscope.  Along with using more accessible imaging equipment...reagents, straightforward protocols, and standard microscopes sounds great, right? But there is always a catch...

...<1%) of authors have asked for advice about the scope of the policy. End of story? Not quite. The PLOS...types of data). Rather, PLOS appears to leave the scope of data to be shared to individual authors. Minimally...to create charts and tables in the paper are in scope. For images, the number of examples supporting the...

...antibody binds that first antibody, then you use a microscope to see where the target protein is (localization...fluorophore that you can image separately on your microscope. Abcam's sample protocol is a good place to get...red-green images generated automatically from the microscope software, you can always recolor them! In Fiji...