... Protocols Using a Light Microscope Using a Light Microscope Intro to the Lab...dish. Microscopes come in a huge range of shapes and sizes - from phone-sized, foldable microscopes that...encounter: the compound light microscope. As the name suggests, light microscopes take advantage of the physical...light microscope with the main components labeled: Figure 1: Diagram of a compound light microscope with...Equipment Light Microscope Prepared Slides (or other sample that can fit on a microscope stage) Reagents...you are using the microscope you may wish to record your observations. Some microscopes have digital cameras...take care of your microscope. Bumps, scratches, and dust can easily impair microscope performance. Be sure...

...mounting medium to the microscope slide. Gently place the coverslip on the microscope slide with the cell...pipettes Rocking platform Tweezers Fluorescent microscope 0.5–10 µL single channel pipette 2–20 µL single...Primary antibody Secondary antibody Deionized water Microscope slide Anti-fade mounting medium Laboratory wipes...side facing down. Observe the cell labeling on a microscope with appropriate fluorescent filters. Tips and...

...incubator Pipet controller Hazardous waste container Microscope Hemocytometer or other cell counter Reagents ...with ~1000 cells. This will help you focus the microscope later when scanning the plate for individual ...Pro-Tip* Use the well with 1000 cells to focus the microscope, then you can easily scan the plate for individual...trypsinizing cells from the 96-well plate, use a microscope to ensure that the cells have detached from the...

...liquid sample Using a Light Microscope Learn about the parts of a light microscope and its use Weighing Reagents...

...Hazardous waste container Inverted fluorescence microscope Reagents DMEM high glucose, Corning 10-013-CV...