...2 Here! We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them...

...has been supported by a Steering Committee and working groups with representatives from all areas of the...

...group in industry? The collaborative nature of working in industry and how delegating can empower people...she sets metrics for success. The importance of thinking about the big picture and not just the next experiment...Chapter 6: Advice to scientists who are planning on working in industry. The importance of recognizing and ...

...genome randomly, while others use attP sequence docking sites so that you can control exactly in which ...at different loci. Fortunately, there are many docking sites available for integration. 2. How soon would...would know beforehand on which chromosome your docking site is located). This amounts to a small difference...important to note so that you know what to look for. Making your transgenic fly and downstream experiments ...experimentation. Drosophila are amenable to many different kinds of experimental assays depending on your interests...University. He has had nearly a decade of experience working with Drosophila as an experimental system. Jon ...Central PMCID: PMC1526508. 2. H. Jiang,  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis...

...importantly, this knowledge will stop you from embarking on a fruitless journey of trying to get males ...that. Now you have the basic knowledge to start working with flies. Like Addgene, the Drosophila community...chromosomes to use. If you’re not ready to start working with live animals yet, Harvard’s DRSC/TRiP- Functional...University. He has had nearly a decade of experience working with Drosophila as an experimental system. Jon ...

...need to be cost and time efficient. We do this by making our own competent cells and using a little-known...efficiency of transformation (for example, if you are working with a library or an especially low concentration...will work just fine.  A number of protocols for making competent cells are available online. A few examples...

...here and you can find more tips and protocols for working with lentivirus on our blog and protocol pages.... love to hear from you if you have used or are thinking about using our viral services. Please send any...

...mouse and human kinomes. While any subset of the genome is limited by definition, the kinome is a well-annotated...genetic screening project can be a tremendous undertaking, producing a wall of results that can only be... About CRISPR Pooled Library Screening Why the kinome? While properly designed perturbations – in the...incremental approach is prudent. What's in the CRISPR kinome library? Thus, we are glad to provide CRISPR libraries...pools provided in two sets, each with 4 gRNAs per kinase, so screens can be performed with either 4 or 8...individual lentiGuide gRNA plasmids targeting the human kinome. These plasmids contain the same 3-4 gRNAs found... Additionally, this set can be used for cherry-picking individual gRNAs that score as hits in pooled screens...

... I found Vectr to be fairly intuitive and I was making figures quickly. Inkscape Inkscape is the most...images. For example, by selecting “Tools” and then clicking the “Usage rights” dropdown menu, you can search...

...fluorescent proteins to oligomerize is known as “stickiness.” Since the fluorescent tag should operate as... per unit.  There are several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration...oligomerization in cells. In addition to measuring stickiness, several researchers have also collected data...characteristics are important, but have less priority than stickiness. In my experience, fluorescent proteins that ...independent of GDI-mediated extraction and actin-based trafficking." PLoS biology 13.4 (2015): e1002097. PubMed ... measurement of Gαi1, Gαi2 and Gαi3 activation kinetics in single cells." PloS one 11.1 (2016): e0146789...

...differently if I would be speaking to a colleague in the office next door. So be kind, helpful, and thorough...

...really fit in anywhere and everyone who was doing the kind of work my lab wanted to do already seemed to know...graciously took me for coffee and spent an hour with me talking about the tools I could use, and some experimental...This was invaluable to me as a bench scientist working at an institution where training opportunities ...network at the meeting. We found our mentors by asking for help with the program on social media. Many...between mentors and Singletons can accelerate the networking timetable for the Singletons in their bins.  ...organizers in their town hall.  Iterative improvement - Making Binning Singletons better Our experience running...leaps. Binning Singletons is its own organization, working to make scientific meetings more productive for...

...University. In this second post in our quick guide to working with Drosophila, you’ll learn how to maniupate ... YFG. You could compare this mutant fly to one lacking the mutation in the same genetic background. In...express YFG in a new tissue type (more details on making a fly line in the next blog post). For example,...get the right cell type expression as opposed to making a new fly with hedgehog under a new promoter each...University. He has had nearly a decade of experience working with Drosophila as an experimental system. Jon ...

...where I spent a couple of days talking to researchers about their work, picking up plasmid deposits, and trying...spend a large amount of my time meeting with and talking to graduate students, post-docs, and PIs. I won...enterprise work better and because I really like talking to people about and communicating science. The ...research at BU, I realized that I’m energized by talking to people about science and that I love explaining...running away screaming, but instead can involve walking alongside it and giving it a high five. While screaming...technical writing, teaching, presenting, analytical thinking, time management, information distillation, etc...transferable to many different fields. If you start thinking about preparing yourself for a career while still...

...business of iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000 clones...efficiency.   But what’s better: PAM-blocking or guide RNA blocking mutations? This depends entirely on...efficiency of guide RNA blocking mutations. So if the locus does not allow blocking the PAM sequence (e.g...intended mutation while taking advantage of the improved efficiency provided by blocking mutations. Essentially... incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our intended... same CRISPR/Cas-blocking mutation but not the intended mutation. Here, the blocking-only templates will...used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes in human induced pluripotent...

...experience working in a developing tropical country. This is why I set up a placement working in a rural...work-life balance compared to working in research. My previous experience working in Industry was really valuable...sustainable. After working at this company for several years, I was ready for a change.  Working abroad: Research...new science graduates and the pro’s and con’s of working in different sectors. Luckily, I have worked for... decide for themselves.  Here is my experience looking for the perfect job. Summer work experience in... explain why I changed jobs. I highly recommend taking on a similar position in your field of interest...and problem solving. I believe that is something lacking during degree courses; they never really prepare...

...room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs have...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...choosing controls, or mixing clear solutions—public speaking is skill that any scientist can learn.  Any time...how to get it, especially if introverted ), and networking (how to get people to remember you). What better...Watch other people give great talks We are not talking about lab meeting here. Lifeless presentation of... Series. Watch how these speakers talk without looking at their slides or notes. Listen to their stories...and policy content. Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion...

...46 : How Kingdom ended up working in the life sciences and agriculture 5:47-9:35 : Kingdom's biotechnology...: Intro 1:01-3:48 : Kingdom Kwapata's background and education 3:49-4:35 : Kingdom on opportunities to...14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44 : Follow up Kingdom's PhD projects...Seeding Labs is helping advance Kingdom's research 28:08-END : Kingdom's Thoughts on his time in Boston...family in Malawi that pushed science education, Kingdom Kwapata grew up to be one of the best of the best...Biotechnology at Michigan State University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata...research at Michigan State University 9:36-10:29 : Kingdom's transition back to Malawi and Lilongwe University...

...presenting on building relationships (also known as “networking”), one of the most common questions I receive...starting to see the advantages of using social networking to start, build, and track professional relationships...professional contact information available. When I am speaking at a new institution, for example, I will often...d be much more likely to say  “Hey, thanks for looking me up on LinkedIn!” resulting in just a little ...inviting someone to connect, perhaps you should be asking yourself if it’s really a good idea.  Pro tip: ... unless you’re on a profile page. You might be looking at a list of suggested connections and you’ll see...complete strangers as it borders on my first rule of networking--”If it feels creepy, don’t do it.” It’s just...

...chat box and upvote If the thought of walking up to the mic and asking your question in a large conference...in case someone’s internet cut out. “ On networking Networking can be more difficult in the virtual format...organic networking,” she says.Depending on the meeting, there may be a “place” for networking within the...answered this way and the hurdle of getting up and asking in front of many people is lower,” says Bachle....,” says Zhang. “Usually the moderator starts by asking the speaker the highly upvoted question.”If the...in the chat box. That’s another benefit to note-taking: you can revisit the Q&A in the chat box. Presenting...the participants to learn about what they were looking to learn in the workshop so they could steer their...