...scientist who began working remotely recently? Most of us at Addgene began working from home last week... mindfulness group is sticking with their Headspace practice even while working remotely. Social distancing...week to flatten the curve, but working remotely can be more difficult if your work is based in the lab. ...sharing links to online yoga classes. Get outdoors. Taking a walk or a bike ride is a great activity for both...the desire and ability to, some communities are looking for bench scientists to assist in labs that are...volunteering to foster a pet, for example. If you are looking for some resources specific to self-care during...check out Care For Your Coronavirus Anxiety and Taking Care of Your Behavioral Health. Work on personal...

... to DNA, crosslinking may not be necessary. This is known as Native ChIP.  If you’re working with a tissue...analysis process. And ChIP itself begins at...  Crosslinking Once you have your sample, the first step is...sample processing and therefore will require crosslinking, which uses formaldehyde or UV light to create...complexes together until you want to break them. Crosslinking is necessary for most ChIP experiences, but ...determine concentration.  Protein Capture After crosslinking and sonication or enzymatic digestion, you’re... not be a good choice) and the amount of cross-linking can affect epitope availability.  Collect the DNA...then incubate with Proteinase K to reverse the crosslinking. Finally, purify your DNA with a PCR purification...

...time for me to start considering my future path. Talking to Addgenies has helped me get a much better idea... Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since he... really get me where I haven’t gotten myself.” Perkins noted that there is a more emphasis today about...those doors to be opened.” Addgenie Eric Perkins presented a poster a few years ago at a CRISPR ...away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community college when she...assistant in a lab at the university that I went to. Making that switch from being a grad student to an employee...Technician, dreamed of becoming a doctor. After taking a year break in between undergraduate school and...

...there's a big office vs. lab culture shock). I'm looking to see if there are any resources that can help...and many of my scientist colleagues have as well. Making the transition from the bench can be jarring for...aren’t a lot of resources out there for scientists making this transition. In this installment of the Addgene.... What causes the lab → office culture shock? Working in an academic research lab involves a few key ...your family. I talked to a graduate student at a networking event recently who referred to her lab as her...applicant asked if she would partake in “critical thinking” in the position to which she was applying. I ... stretch of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch says, “[It] seems...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where... cell culture, I would often respond to friends asking me to dinner saying something like, “I’ll be there...ve learned a lot since my days as an undergrad working with cancer cell lines. As I started planning out... this post useful. After a bit of research and thinking about my own processes, I’ve broken down time ...until it was too late. I didn’t spend enough time thinking about the possibilities and how to prioritize ...the day, month, or even the year, you should be thinking about two main things: How useful the task is...time it will take you to complete the task When picking which tasks to actually do, you should err on the...

...afford them). I am talking about making sure the audience is interested, educated and talking as a result of...moderator should have a well-planned approach to making the event interesting and engaging. Long before...the pre-event interviews, the moderator should be taking good notes and discovering interesting stories,... panelists have the correct date, location and parking information and remind them about the event as ... a really lively discussion, I strongly suggest asking the panelists not to use any slides for the event...prevent any one panelist or audience member from taking over. My own personal rule is that I do not let...chose good panelists, they are going to be good at talking, so they will easily take 5 minutes to introduce...

...conference. Networking All of the things we've mentioned above could be considered networking, but here ...tips. Before the conference Networking is key even if you are not looking for a job - those connections...the elevator pitch, talks, the poster session, networking, and social media. The elevator pitch Before...poster or talk at the conference, you can end by asking them to come to your poster or talk. After the ...bored, or particularly excited about? If you tried tweaking your pitch throughout the conference, what worked...the poster session, send them a follow up email thanking them for their conversation and connect with them...social media. Social media Before the conference Networking can be made easier through social media. Use ...

...human kinases remains challenging. Small molecule kinase inhibitors usually target soluble kinase domains...Human kinase domain constructs for automated bacterial expression The misregulation of human kinases has...recently compiled the Human Kinase Domain Constructs Kit, a library of human kinase domain constructs developed...Threonine kinases while YopH (residues 164-468, Plasmid 79749) enhances expression of Tyrosine kinases. All...Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking autophagy in yeast with Rosella, a pH-sensitive...a target cell. In addition to nucleotide-based linking strategies, post translational protein fusion would...Arumugaswami lab at Cedars-Sinai Medical Center has kindly constructed and deposited an unpublished set of...

...cutters. You can also sort enzymes by clicking on column heads. Clicking on a particular restriction enzyme...plasmid maps!" After thoughtful design, vetting, and tweaking, we’re excited to announce that our plasmid and...GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software and extensive...identities of most features labeled directly on them making them much easier to quickly identify. Improved ...With this more functional display, you can start thinking about your next cloning experiment early on. Beyond...Maps This is the first tab you’ll land on after clicking the “Analyze Sequence” button. The maps hosted...similar hover over features. As with the map tab, clicking on two separate locations in the linear sequence...

... and then condensing that and making it accessible to students.” Making science approachable is often ...This involves going in front of a camera and kind of walking them through the steps of, for example, cutting...And I’m like, 'Well, I’m here so let’s do it!'" Working as a subject matter expert Caitlin started out ...job as an SME: “I write curriculum for students taking science courses online, which is a blast. I have...an introduction that gives a basic overview of kinematics in physics, to developing a lab about mitosis...eScience Labs as part of their curriculum by either taking existing curriculum and customizing it for schools...courses encompass what they want to teach.” Besides working with students and faculty, Caitlin also collaborates...

...basic one, making sure that you have a good fundamental understanding of what you’re working on is important...years. We each have a unique “origin story,” but taking the first step into the world of academic research...glassware with brightly colored hazardous liquids. Working in a lab is nothing like a typical part-time or... and applied. The current pandemic serves as a striking example of the applicability and importance of...be unsure, it helps to have a vague sense of what kind of research you would be excited about. I know quite...do what they do. I would also highly recommend talking to other undergraduate researchers, as well as ... yourself into is really important.  As you’re talking to people, though, try to be conscious of who has...

...thawed and used to produce a set of ‘working’ stocks of cells. Working stocks should be frozen in multiple... generally ensure cell line identity and purity making it easier for comparable studies to be performed...to check their identity. For example, perhaps a working cell bank has been made by a research institution... good recordkeeping, you need to have a way of tracking how many passages the cells have undergone and... any of the Master cell vials until all of the working vials have been used up. This ensures you can retain...means you can regularly discard the cells you are working with because you have plenty of maintained freezer...freezer stocks. It is vital that the Master and Working stocks of cell lines have the passage number recorded...

...available for tracking certain cell types. Another useful tool for marking and tracking cells is the the...tools allow one to study the kinetics of cell migration and movement by marking cells at a specific time.... Pathogens - Kamenyeva et al, 2015. Cellular trafficking studies enabled by fluorescent proteins The optimization...tissues, including lymph nodes, brain, liver, gut and skin. Two-photon intravital microscopy. The video above...that some are more reliable and more efficient at tracking cells. Good two-photon probes when using a standard... cells and follicular dendritic cells within the skin and lymph node respectively. The permanent labelling...photoactivable or photoconvertible proteins, as it enables tracking throughout the lifetime of the cell.     The past...

...qualify what kind of “plate” you mean. Without context, it’s difficult to know what kind of plate they...professionals from other backgrounds at conferences or networking events. And if you’re a wet lab scientist who...collaborative lab work. Corralling context and junking jargon I suspected I knew at least one source of...they were talking about during the meetings. And “plate” certainly wasn’t the only problematic word. How...We took steps towards solving this problem by 1) making sure we qualified these words when we discussed...and racks meant we had fewer errors in our LIMS tracking system. Though you might not have to communicate...creating shared experimental protocols. Clarify: Asking for clarification during a meeting can help get...

...release IL-36 cytokines. Keratinocytes are the main source of IL-36 cytokines in the skin making it important...the uncontrolled activation of the interleukin-36 (IL-36) cytokine family resulting in the activation ...disease research. This year is our fourth year working with the Rare Genomics Institute by providing plasmid-based...pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6 per 10,000...surrounding IL-36 biology. “Little is known how IL-36 cytokines actually get out of keratinocytes and induce their...researcher at University Hospital Zurich. IL-36 cytokines are a potent inducer of an autoinflammatory loop... our biosensor repository as we find ourselves lacking several crucial biosensors that would allow us ...

... worth taking time to honestly evaluate your own style of work and communication before making this important...attention you need? Are you good at asking for help? If you are good at asking for and getting help, size might... toss data-driven reasoning out the window when making decisions. We think scientists make choices based...based on emotions and assumptions. I’m not just talking about simple things like “Which route should I ...members can give you a fuller picture. Consider talking to people in the lab across the hall or in the ...prematurely? Do lab alumni stay in the same field taking projects with them and remaining close colleagues...t choose a mentor that sees you only as a paper making machine. Choose one that is a true mentor, supporting...

...yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...type of team can exist indefinitely. If you’re looking for this type of team in academia, look no further...an imaging core or tissue culture core, you are looking at a process-oriented team serving a particular...you find that there is not someone (or someones) taking on these responsibilities on a team to which you...is certainly necessary, but equally important is making sure all team members understand what roles they... none of that matters. If you are on a team, any kind of team, and you have nagging doubts that someone...

...of my job], so making it a success feels like a real accomplishment.” He started working on them shortly...not sure what his next step should be. He debated taking a position as an application support/sales representative... to me. She was leaving a writing position and looking for someone who might take over. I like writing... a science writer these days. I do some public speaking and a bit of video work for webinars (get tips...shortly after joining IDT when his boss was looking for someone to take over hosting them. Since then, webinars... writing group I am part of is responsible for checking the scientific accuracy of everything that goes...analysis.” Han’s biggest piece of advice for those looking to break into the field of science communications...

...back out when you are looking to change positions in the future. Anyone looking for work in today’s job...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...student or postdoc forever, and you need to be thinking about what comes next. To that end, one of the...for your career is to expose yourself to other working professionals, and one way to do this is to attend...and connections are how you get jobs! Job fairs Speaking of jobs, it seems like an obviously important ...conference, job fairs bring you face-to-face with working professionals, with the added benefit that most...larger meetings offering resources for students seeking employment within industry or academia; although...

...recognize their MANY transferable skills learned while working in the lab and offering advice on areas where you...using creative problem-solving in the lab   Thinking Outside the Box: Individual Approaches Whether...something tangible and applicable to your situation.   Thinking Outside the Box: Team Approaches At some point...same problem. If your lab/company/team is still working towards a culture of encouraging dissent, the next...nine enemies that stand in the way of creative thinking. I won’t go through all nine here, but I’ll discuss...longest tenure, but don’t let that stop you from speaking up.  If your ideas are not well received, understand...work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible...