...optimist, I’m a realist, and together we make a pretty good team. We have confidence that we can take ...to a SynBio startup At some point, our ideas got pretty good. We started doing extracurricular research... successful business that the learning curve is pretty steep. But we can rely on one another to celebrate...

...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...cleaves relatively far away from the PAM, NHEJ might retain the PAM site. Therefore, if HDR does not initially... FJ, Terns RM, Terns MP, White MF, Yakunin AF, Garrett RA, van der Oost J, Backofen R, Koonin EV. An updated...

...pipetting, as shown in the YouTube video. Before returning the plate to the incubator, additional growth ...fluorescent protein), fluorescence microscopy can theoretically be used to select specific wells from the 48...SH-SY5Y cells, as these cells did not seem to form discrete colonies but constantly migrated away from each...

... the reprogramming factors were delivered via retroviral infection [8]. The work was taken forward the...generation of human iPSCs from dermal fibroblasts via retroviral and lentiviral infections respectively [9, 10... 350(13): p. 1353-6. PubMed PMID: 14999088. 7. Turetsky, T., et al., Laser-assisted derivation of human...

...protocols, and more. Selecting crispr in Guides will return links including our CRISPR guide.  For a closer...What type of experiment are you planning? (e.g. Retroviral) Species: Specify the source organism for a gene... plasmids. A search for “RNA editing” plasmids returns more than 1300 results, and applying a filter for...

...include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent...goal, what you expect to find, and how you will interpret and use the information. In contrast, a list of...

...developed a split intein spCas9-AAV toolbox that retains the gene-targeting capabilities of full-length ...10.1038/nmeth.3993  Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ...Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated...

...2014, the White House issued a memorandum to the Secretary of Labor to update the FLSA, which had not seen...automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime regulations...post by Director of the NIH Francis Collins and Secretary of Labor Thomas Perez, Fair Pay for Postdocs: ...

...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...time to start answering questions. r/Science has pretty strict rules about not being too sales-y in posts...questions from the AMA - as you’ll see below, it pretty nicely displays her energetic, truthful and helpful...

...support, but there are eight other teams who still retain a customer support element amongst their other ...The first and foremost sub-skill to have here is pretty intuitive: You need to be willing to face conflict...this skill to another job, it will be important to retain the stories and examples that have guided your ...

...copper cathode/iblot gel matrix, and filter paper. Pretty much like an actual sandwich! S'mores image from....2015.04.012 Burnette WN (1981) “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide....76.7.3116 Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels...

....     Pro-Tips: 1. It’s all about the MOI. In retrospect I used a way too high Cas9 MOI considering that...I didn’t do this, but to do it you would need a pretty good gel image. If you are analyzing short DNA ...cancer cell line in about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research...

...high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic...

...experience. Working in industry: Microbiology On returning from Ghana at the beginning of 2010, I explored...looking for other career options after 6 months. Returning to university to study a Masters of Science I ...programming. Working for a scientific nonprofit On returning to the UK, I checked out the job postings from...

... (a British idiom for “taking by surprise”) by Margaret Robinson’s lab, and more recently used by Stephen...Opto-RTK constructs – Opto-mFGFR1, Opto-hEGFR, and Opto-hRET – as well as various LOV-domain-mVenus constructs...directionally assemble in a single reaction, while retaining only a defined 4bp fusion site in between. The...vectors with the desired sequence of interest are retained after SLIC cloning. The general cloning strategy...

... was not present (Kuhlman, SK. 2008). While theoretically this strategy should work, these vectors suffered...data and can instill greater confidence when interpreting the outcome.    References and resources References...Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for...

...proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent protein tools. Future developments...Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

...drive change. This effort is already resulting in concrete actions and outcomes. For example, pd|hub assembled...collections of evidence-based practices. These concrete programs and practices to promote PhD professional...

...me to join the committee because I had recently retired from Harvard, and it let me keep my toe in the ...little bit?”    What are you doing to enjoy your retirement? I’m also on the advisory board for Art in Giving...

...lungs are difficult, but other tissues like the retina have cell-based therapies underway. Chapter 7: ...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from...