...what constitutes the “appropriate” codon is not necessarily obvious. You might think, “Nonsense! I should... above, not every region of a protein should necessarily be translated rapidly to produce a protein that...optimize a gene with one of these tools, it doesn’t necessarily mean the gene is going to express well. If you...

...which contains the regulatory regions that are necessary for myosin activity and thus interaction with ...actually a Drosophila meiotic arrest gene that is necessary for spermatogenesis. Flies that lack cookie monster...spatially restricted to the dorsal mesoderm, is necessary to activate bagpipe which is localized to segmental...

...training behind you (and largely only that is necessary), there are opportunities to work for biotechnology...their technical support, and a PhD is not always necessary. The job description and the candidate requirements...empathetic, and tactful. Occasionally it can also be necessary to take the lead and be assertive. You develop...

...abundance, and thus difficult to purify in quantities necessary for study. Moreover, the ability to isolate purified...strain for pSer-incorporation The second component necessary for phosphoserine incorporation is a modified ...interest as possible, some optimization will be necessary. To begin, one should optimize protein production...

...eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science paper. You do need...can suffocate an exciting idea in a swamp of unnecessary details. You DO NOT need to present a detailed...an interview, your previous success will not necessarily translate into getting a job. Your papers and...

...enough funds to get started, wasting funds on unnecessary materials, or even a loss of the offer all together...usually focus on the numbers, rather than the necessary resources. This is why at Experimental Designs...too often, we see early investigators making unnecessary short term sacrifices that hurt their goals, ...

...withholding necessary information Overwork – including undue pressure, impossible deadlines, unnecessary disruptions...undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation...

...reproducibility. For a study to be “reproducible” does not necessarily mean that someone has replicated it precisely...them upon completing an experiment. There’s not necessarily in-principle acceptance, but readers of pre-registered...practices including talking to statisticians when necessary and encouraging interlab peer review of experimental...

...and validation Can’t forget the controls! The necessary control experiments will vary depending on your...standard IF. Here, we’ll walk you through the necessary types of controls for a multiplex experiment.  ...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M...

...), quantifying antibody/antigen interactions (Saraheimo et al., 2013), and visualizing structural changes...FRET acceptor labeling specificity is not always necessary for successful FRET experiments. If the acceptor...40.3 (2006): 279-286. PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach...

...Unearthed Comics” by Sara Zimmerman   5)   “Beatrice the Biologist” by Katie McKissick       Sara Zimmerman, Unearthed...

...bad mentor. Apparently, a good Jedi does not necessarily an excellent mentor make. Are your current mentors...developing colleagues even as I reinforce the necessary power dynamic between advisor and students.” Stay...

... special year for Addgene: it marks our 20th anniversary. Throughout the year, we’ll be celebrating with... You can enjoy these stories, and other 20th anniversary celebrations, by checking out our blog, social...

...spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad control of whole circuits... circuits or tissue-wide systems is necessary, use chemogenetics. Note that, as with all tools, both approaches...

...scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology article “Managing knowledge-intensive...Have their own working schedules and may not necessarily be comfortable with imposed deadlines (there’...

...uncertainty and precautionary regulation was all necessary to inform the experimental aspect of my thesis...its own right, science communication reflects a necessary confluence of science and the world it hopes to...

...fibers would need to be, as well as other features necessary for implantation in brain tissue. The gut, on...relatively small, and can be detached easily whenever necessary. This is all an impressive engineering feat, ...

.... The double stranding step was assumed to be necessary because the AAV genome is single-stranded and ...this double stranding step might not actually be necessary. Since the AAV genome exists as [+] and [-] strands...

...pending legal agreement ever delayed your access to necessary research materials? If so, you should always talk...Whitehead Institute for Biomedical Research Glossary Master Deposit Agreement: An agreement, executed...

...higher titer than others. High titers are often necessary to visualize expression of fluorescent proteins... get a good fluorescent signal. It may not be necessary to concentrate this type of vector to such a high...