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Showing: 61 - 80 of 251 results

  1. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...believe people who tell you that thank you isn’t necessary. I once got a box of 6 perfect, chocolate-covered...connections on a regular basis. It is also not at all necessary. Once you’ve talked with a person a few times,...

  2. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...interactions and #social support are even more necessary during these times of stress due to #COVIDー19.... virtually. With co-working, the idea is not necessarily to chat, but to mimic your usual work environment...

  3. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ... at times."     - Jason Snair, Product Designer Sarum: The Novel of England by Edward Rutherfurd "A 10,000...Following history from the Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five...

  4. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...higher titer than others. High titers are often necessary to visualize expression of fluorescent proteins... get a good fluorescent signal. It may not be necessary to concentrate this type of vector to such a high...

  5. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...This individual protein has all the components necessary to: 1. Bind to a guide RNA The guide RNA, which...specific genomic locus and the scaffolding sequence necessary for Cas9 binding. When using CRISPR/Cas9 for genome...

  7. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...until you want to break them. Crosslinking is necessary for most ChIP experiences, but the increased binding...quite strongly to DNA, crosslinking may not be necessary. This is known as Native ChIP.  If you’re working...

  9. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...see a name or gene on this list, that does not necessarily mean the repository does not have it. That said...structure of paraspeckles. Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess A, Lawrence JB Mol...

  10. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...plasmids used in the lab typically contain all the necessary components to allow shuttling between E. coli ...transformation efficiency; however, this region does not necessarily promote replication.  Selection markers for yeast...

  11. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...surface, but it’s quite easy to add in “necessary” detail after “necessary” detail and end up completely overwhelming...

  13. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...targeted autophagy of mitochondria. As shown in Sargsyan et al. It is also possible to make Rosella fusions...doi:10.4161/auto.5331. PubMed PMID: 18094608 2. Sargsyan, A., Cai, J., Fandino, L. B., Labasky, M. E., ...

  14. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...calcium binding sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity...great FRET sensor option if you have the setup necessary for readout.   We’ve got a live one ...

  15. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...sequence like a known backbone sequence. We’re not necessarily expecting a perfect match - we will often find...insert of the library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well...

  16. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...into viral genome, so viral progeny will not necessarily express EnvA unless it is supplied in trans. ...phenomenon has shown that the RABV G protein is necessary and sufficient for retrograde transmission (Etessami...

  17. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...way it is funded and to learn tangible skills necessary for affecting change. On Friday, the symposium...designed to explore areas where further data is necessary to determine the best solutions. The event also...

  18. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...with these sensors, but further optimization is necessary for these to be as effective at the single cell...perform poorly in two-photon conditions, which are necessary conditions for penetrating deeper tissues depths...

  19. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... have the power to take the added precautions necessary to keep your cells from becoming contaminated....you’re wearing before you start. Also, avoid unnecessary talking because talking generates microbe-laden...

  20. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...Because the consequences of such a mistake would necessarily extend far beyond the laboratory and seriously...inherently collective technology: because they necessarily alter the shared environment, they must never...
Showing: 61 - 80 of 251 results